Research of the yeasts Candida non - albicans on various medical devices in the service of resuscitation of the Teaching hospital of Tlemcen, Algeria

Background and aim: The fungic infections, more particularly the Candidiasis are frequent in the intensive care units. This had a relation with the heaviness of the pathologies, presented by the patients and the quasi-systematic recourse to various medical devices. In addition, we observe more and more and more the emergence of numerous species of Candida non- albicans in these hospital structures. That’s why we undertook this study which concerns the evaluation of the incidence of the sepathogenic on medical devices implanted during 48hours and  more on patients hospitalized in the resuscitation service of CHU of Tlemcen.  Methods:After isolation of yeast son selective mediums, the strains obtained were  identified by using chromogenicmediums (CHROMagar®Candida) and the Api identification Candidagalleries (BioMérieux®). Results:The results showed that among100 samples are taken, 15% been altered by yeasts of Candida non-albicans. Conclusion: It appears from this study that the fungal infections on medical devices are widely present in hospitals especially in the intensive care unit. The presence of Candida non- albicans yeasts is not negligible; they are 15% on the level of the various medical devices implanted

The size of catheters: an important parameter to consider in assessing infectivity

The diagnosis of catheters’ infectivity is established considering several parameters. These relate to the clinical patients’ data and the microbial load of the catheters following their culture. Catheter infectivity type is related to the significance threshold. However, differences in sizes exist between several catheters. So, in order to qualify any microbial alteration, it is important to take into account the impact of the size of the catheters. For this, future studies should consider this parameter to assess microbial load properly

Medical and epidemiological impact of candidal biofilms, tridimensional architecture, and resistance

A hospital can be considered an ecosystem where the patient is found in contact with the microbial world and faces the risk of contracting an infection that is termed the nosocomial. Some opportunist pathogens yeast parts of this universe, candida sp., is responsible for more than 75% of systemic fungal infections. These infections are primarily related to medical devices such as catheters that promote the formation of biofilms. That structure sets up nests for disease because it is not easily amenable to conventional antifungal therapy. The diagnosis of catheter-related candidiasis is difficult; however, the differentiation between catheter infection and a simple contamination is essential to establishing an antifungal treatment. In this context, we conducted our study between February 2011 and January 2012 at the Hospital University Center of Sidi Bel Abbès (Algeria), which is to assess the responsible yeast species, then, to check their power to form biofilms and to test their resistance against amphotericin B and fluconazol. From 457 samples, 37 strains of candida sp. were isolated, along with the dominance of C. glabrata. Nevertheless, 31 strains were able to form biofilms; in addition, it appears from this study that the antifungal tests clearly show that sessile cells of candida sp. were much more resistant than their planktonic counterparts (32 times higher toward AmB and 128 times to fluconazole). Moreover, images of electron microscopy show the formation of biofilms on the internal surfaces of catheters

Polyphenols Content and Antimicrobial, Antioxidant and Hemolytic Activities of Essential Oils from Four Selected Medicinal Plants Growing in Algeria

The Saharan and steppe spontaneous plants are very characteristic because of their particular adaptation to the desert and extreme environment. Some species have pharmacological properties that give them a medicinal interest. The aim of the present work was to determine the polyphenol contents of essential oils obtained from four endemic plants growing in Algeria (Pituranthos scoparius, Myrtus nivellei, Rosmarinus officinalis and Mentha piperita), and study its biological activity, including antimicrobial, antioxidant, and hemolytic. The antimicrobial activity was evaluated by the microdilution method against twelve strains. The antioxidant activity was carried out by two methods (DPPH radical scavenging and reducing power). However, the hemolytic effect has been evaluated against the red blood cells. P. scoparius and M. piperita showed yields of essential oils higher than 1%. All the strains showed sensitivity against the essential oils tested with the exception of the C. albicans treated by R. officinalis essential oils. The most sensitive strain was C. albicans treated by P. scoparius essential oils by MIC of 0.0781 mg/mL, it was the same plant that shows the highest polyphenol content (14.78 ± 0.72 g GAE/g DS). The antioxidant activity by the DPPH method was greater for all essential oils tested by IC50 ranging from 0.69 ± 0.07 (R. officinalis) to 30.67 ± 2.12 mg/mL (M. nivellei). The R. officinalis essential oils reported more antioxidant power than the positive control (ascorbic acid). In reducing iron, it was the R. officinalis essential oils which were found to be the most active with an EC50 concentration of 9.67 ± 1.36 mg/mL. After 120 min incubation, minimal haemolysis (10%) was obtained with essential oils of R. officinalis at a concentration of 0.39 mg/mL. We conclude that P. scoparius essential oils showed the high content of polyphenols and R. officinalis essential oils reported more antioxidant power than the positive control (ascorbic acid)

Effects of tyrosol and farnesol on Candida albicans biofilm

The present in vitro study examined the effects of the quorum‑sensing molecules farnesol and tyrosol on the development of Candida albicans biofilm in order to elucidate their role as novel adjuvants in oral hygiene. The investigation was conducted in C. albicans ATCC 10231 and C. albicans isolates from dentures and was performed in flat‑bottomed 96‑well polystyrene plates. Yeast growth and their capacity to form biofilms were evaluated following 24 and 48 h incubations at 37˚C in Sabouraud broth supplemented with 0.001‑3 mM farnesol and/or 1‑20 mM tyrosol. Yeast growth was assessed by turbidimetry and biofilms were quantitated by crystal violet staining, under aerobic and anaerobic conditions. The viability of the fungal cells was controlled by the culture of planktonic cells and by examination of the biofilms using fluorescence microscopy following staining with fluorescein diacetate and ethidium bromide. Farnesol at 3 mM exerted a stronger action when added at the beginning of biofilm formation (>50% inhibition) than when added to preformed biofilms (80% inhibition) than on preformed biofilms (<40% inhibition). Despite significant reductions in attached biomass, yeast growth varied little in the presence of the investigated molecules, as corroborated by the turbidimetry, culture of supernatants on solid culture medium followed by counting of colony‑forming units and viability tests using fluorescence microscopy. At the highest tested concentration, the molecules had a greater effect during the initial phases of biofilm formation. The effect of farnesol during anaerobiosis was not significantly different from that observed during aerobiosis, unlike that of tyrosol during anaerobiosis, which exhibited slightly reduced yeast biofilm inhibition. In conclusion, the present study demonstrated the specific anti‑biofilm effect, independent of fungicidal or fungistatic action, of farnesol and tyrosol, as tested in C. albicans ATCC 10231 and 6 strains isolated from dentures. Prior to suggesting the use of these molecules for preventive purposes in oral hygiene, further studies are required in order to clarify the metabolic pathways and cellular mechanisms involved in their antibiofilm effect, as well as the repercussions on the oral microbiome.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Effect of lysozyme on Candida biofilm formation

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Dose‑dependent effect of lysozyme upon Candida albicans biofilm

The present study investigated the in vitro effect of lysozyme (0‑1,000 μg/ml) on Candida albicans (C. albicans) biofilm development. Investigations were conducted on C. albicans ATCC 10231 and on 10 clinical isolates from dentures. Strains were cultured aerobically at 37˚C in Sabouraud broth. Yeast growth was evaluated by turbidimetry. Biofilm biomass was quantified on a polystyrene support by crystal violet staining and on acrylic surfaces by counts of colony forming units. Lysozyme affected biofilm formation to a greater extent than it affected growth. For the ATCC 10231 reference strain, lysozyme acted as a biofilm promotor on polystyrene at the highest concentration tested (1,000 μg/ml, non‑physiological). When the reference strain was investigated on acrylic resin support, lysozyme acted as a significant biofilm promotor on rough resin, but less on smooth resin. The attached biomass in the presence of physiological concentrations of lysozyme (10‑30 μg/ml) was significantly decreased compared with the hypothetical value of 100% using a one‑sample t‑test, but a comparison between the different lysozyme conditions using analysis of variance and post hoc tests did not reveal significant differences. In 10 wild strains, different patterns of biofilm formation on polystyrene were observed in the presence of lysozyme. Some strains, characterized by large amounts of biofilm formation in the presence of 1,000 μg/ml lysozyme, were poor biofilm producers at low concentrations of lysozyme. In contrast, some strains that were poor biofilm producers with a high lysozyme concentration were more inhibited by low concentrations of lysozyme. The present study emphasizes the need to develop strategies for biofilm control based on in vitro experiments, and to implement these in clinical trials prior to approval of hygiene products enriched with exocrine proteins, such as lysozyme. Further studies will extend these investigations to other Candida species, and to fungi and bacteria present in oral biofilms.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Dietary Patterns and Oxidative Stress in a Population of Women with Breast Cancer in the North-Western Regions of Algeria (Saida and El-Bayadh)

Objective: The aim of this work is the study of risk factors for breast cancer in a population of women in the north-western regions of Algeria (Saida and El-Bayadh) Methodology: A case-control study was carried out on 40 women including 20 cancerous women and 20 control women, in order to determine the dietary patterns and general characteristics of the population studied and to compare the hematological and biochemical parameters, and markers of oxidative/antioxidant status between control women and breast cancer women. Results: The results obtained show that there is no significant difference in the average age however the body mass index was significantly increased in cancer women, revealing overweight (p &lt;0.05), compared to control women. The marital status of the target population is predominantly a married woman status and the level of education shows a relatively low level in breast cancer cases compared to women controls. The illiteracy rate is exceptionally high in cancer women compared to controls (35% versus 20%). In addition, the levels of glucose, creatinine, HDL and ALAT are similar between the two groups of women. The contents of urea, cholesterol, triglyceride, LDL, ASAT, malondialdehyde and uric acid are significantly increased in women with breast cancer compared to women controls. But the vitamin C content is significantly reduced in cancer women compared to control women. Conclusion: We conclude from this study that there is an association between breast cancer and lipid alteration, oxidative stress, age, increase in BMI, socio-demographic data, professional situation, age, means of menstruation and menopause, and ultimately the lifestyle. All these factors are risk factors for breast cancer in the region of Saida and El-Bayadh. Keywords: Breast Cancer, Risk Factors, Dietary Patterns, Oxidative Stress

Ex vivo yeast-decontamination of denture by H2O2/iodide/lactoperoxidase system: need to overpass the microbial H2O2 catabolism

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