Effect of green tea on inflammation and oxidative stress in cisplatin-induced experimental liver function

Introduction: Cisplatin is one of the most potent chemotherapeutic antitumor drugs. Also, oxidative stress has been established to be involved in cisplatin-induced toxicity. Therefore, the present study was undertaken to examine the antioxidant and anti-inflammation potential of green tea hydroalcoholic extract (GTE) against the liver function of cisplatin in male rats.Methods: Adult male Wistar rats (180–250 g) were divided into 4 groups (n = 5) treated as follows: (1) control group (saline solution, 1 ml kg−1 body weight, i.p.), cisplatin group (7 mg kg−1 body weight, i.p.). Animals of Groups III received only green tea extract (30 mg/kg/day, by gavage). Group IV was given green tea extract+ cisplatin once daily for 24 hours. Liver function was evidenced in the cisplatin group by the increased serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). The mechanism of cisplatin induced liver function was considered as being decreased the total antioxidant power (TAP). Systemic inflammation was assessed by tumor necrosis factor-alpha (TNF-α) levels.Results: A decrease in TAP level in cisplatin group was observed compared with control group. GTE administration decreased TNF-α and increased TAP compared to cisplatin group, but showed no significant differences between groups.Conclusion: The results suggested that green tea could ameliorate cisplatin liver function in rats through reduction of oxidative toxic stress and inflammation

Protective role of green tea on malathion-induced testicular oxidative damage in rats

Objective: To examine effects of total green tea extract, a potent free radical scavenger on testicular tissue oxidative status. Methods: 32 male albino rats of Wistar strain were divided into four groups, every group restricted 8 animals: (i) control rats; (ii) green tea-treated control rats; (iii) malathion rats; (iv) malathion-treated green tea rats. Animals received malathion 150 mg/kg and green tea 30 mg/kg for 24 h intraperitoneally. At the end of the treatment period, rat testis tissues were quickly removed and analyzed. Diameter of seminiferous tubules and germinal cell thickness, spermatogonia sertoli cells, primary spermatocytes, spermatids and leydig cell were evaluated. Also, oxidative stress evaluation was conducted based on total antioxidant capacity (TAC), lipid peroxidation (LPO) and total thiol molecules (TTM) in homogenate testis tissues. Results: The results showed that total green tea extract improve oxidative damages against malathion group. Light photomicrograph of seminiferous tubules in malathion-treated group showed noticeable reduced height of germinal epithelium and disorganization of the tubules. An increased intestinal tissue was also observed. Primary spermatocytes were located distance from basal lamina indicating it induced damages to the intestinal tissue. While seminiferous tubules in malathion exposed and green tea extract-treated were normal. Conclusion: This study demonstrated the effectiveness of TGTE on oxidative stress and testicular tissue damage induced in malathion in infertility disorders