Effects of experimental nitrogen fertilization on planktonic metabolism and CO₂ flux in a hypereutrophic hardwater lake

<div><p>Hardwater lakes are common in human-dominated regions of the world and often experience pollution due to agricultural and urban effluent inputs of inorganic and organic nitrogen (N). Although these lakes are landscape hotspots for CO₂ exchange and food web carbon (C) cycling, the effect of N enrichment on hardwater lake food web functioning and C cycling patterns remains unclear. Specifically, it is unknown if different eutrophication scenarios (e.g., modest non point vs. extreme point sources) yield consistent effects on auto- and heterotrophic C cycling, or how biotic responses interact with the inorganic C system to shape responses of air-water CO₂ exchange. To address this uncertainty, we induced large metabolic gradients in the plankton community of a hypereutrophic hardwater Canadian prairie lake by adding N as urea (the most widely applied agricultural fertilizer) at loading rates of 0, 1, 3, 8 or 18 mg N L^-1 week^-1 to 3240-L, <i>in-situ</i> mesocosms. Over three separate 21-day experiments, all treatments of N dramatically increased phytoplankton biomass and gross primary production (GPP) two- to six-fold, but the effects of N on autotrophs plateaued at ~3 mg N L^-1. Conversely, heterotrophic metabolism increased linearly with N fertilization over the full treatment range. In nearly all cases, N enhanced net planktonic uptake of dissolved inorganic carbon (DIC), and increased the rate of CO₂ influx, while planktonic heterotrophy and CO₂ production only occurred in the highest N treatments late in each experiment, and even in these cases, enclosures continued to in-gas CO₂. Chemical effects on CO₂ through calcite precipitation were also observed, but similarly did not change the direction of net CO₂ flux. Taken together, these results demonstrate that atmospheric exchange of CO₂ in eutrophic hardwater lakes remains sensitive to increasing N loading and eutrophication, and that even modest levels of N pollution are capable of enhancing autotrophy and CO₂ in-gassing in P-rich lake ecosystems.</p></div