Construction and Mechanism of Action of Gelatin/Sodium Hexametaphosphate/Glutamine Aminotransferase Based Composite Hydrogel System

In this study, a composite hydrogel system was constructed by cross-linking of primary network hydrogels of gelatin (GE) and sodium hexametaphosphate (SHMP) by transglutaminase (TGase) after addition of Lactobacillus plantarum in order to improve its viability and bioavailability. The experimental results showed that the modification by SHMP and TGase changed the gel strength, water distribution state, and gel network structure of gelatin, and reduced the gelation rate, so that the three-dimensional network structure of the gel was more stable, and the intermolecular forces of the composite hydrogel was stronger, contributing to the resistance of the encapsulated L. plantarum to adverse environments. The presence of L. plantarum was found to slightly disrupt the ordered structure of the hydrogel by scanning electron microscopy (SEM). Endogenous fluorescence spectroscopy analysis showed that addition of L. plantarum resulted in the exposure of the extended region containing tryptophan within the GE molecule to a more polar environment. The steric effect occurred during the gelling process, delaying the formation of covalent crosslinks and physical interactions between the biopolymer molecules, which led to changes in their microstructure. Simulated gastrointestinal digestion tests and storage tests showed that L. plantarum encapsulated in GE/SHMP/TGase gels had better survival rates and gastrointestinal release properties compared to single GE-based hydrogels. It was confirmed that GE/SHMP/TGase hydrogels had a better protective effect on L. plantarum. In conclusion, this study has explored a new method for preparing GE-based hydrogels as a delivery system for probiotics, which will provide a theoretical basis for the development of probiotic functional foods

Translocase of the Outer Mitochondrial Membrane 40 Is Required for Mitochondrial Biogenesis and Embryo Development in Arabidopsis

In eukaryotes, mitochondrion is an essential organelle which is surrounded by a double membrane system, including the outer membrane, intermembrane space and the inner membrane. The translocase of the outer mitochondrial membrane (TOM) complex has attracted enormous interest for its role in importing the preprotein from the cytoplasm into the mitochondrion. However, little is understood about the potential biological function of the TOM complex in Arabidopsis. The aim of the present study was to investigate how AtTOM40, a gene encoding the core subunit of the TOM complex, works in Arabidopsis. As a result, we found that lack of AtTOM40 disturbed embryo development and its pattern formation after the globular embryo stage, and finally caused albino ovules and seed abortion at the ratio of a quarter in the homozygous tom40 plants. Further investigation demonstrated that AtTOM40 is wildly expressed in different tissues, especially in cotyledons primordium during Arabidopsis embryogenesis. Moreover, we confirmed that the encoded protein AtTOM40 is localized in mitochondrion, and the observation of the ultrastructure revealed that mitochondrion biogenesis was impaired in tom40-1 embryo cells. Quantitative real-time PCR was utilized to determine the expression of genes encoding outer mitochondrial membrane proteins in the homozygous tom40-1 mutant embryos, including the genes known to be involved in import, assembly and transport of mitochondrial proteins, and the results demonstrated that most of the gene expressions were abnormal. Similarly, the expression of genes relevant to embryo development and pattern formation, such as SAM (shoot apical meristem), cotyledon, vascular primordium and hypophysis, was also affected in homozygous tom40-1 mutant embryos. Taken together, we draw the conclusion that the AtTOM40 gene is essential for the normal structure of the mitochondrion, and participates in early embryo development and pattern formation through maintaining the biogenesis of mitochondria. The findings of this study may provide new insight into the biological function of the TOM40 subunit in higher plants

Differences in the pathogenicity and molecular characteristics of fowl adenovirus serotype 4 epidemic strains in Guangxi Province, southern China

Starting in 2015, the widespread prevalence of hydropericardium-hepatitis syndrome (HHS) has led to considerable financial losses within China’s poultry farming industry. In this study, pathogenicity assessments, whole-genome sequencing, and analyses were conducted on 10 new isolates of the novel genotype FAdV-4 during a HHS outbreak in Guangxi Province, China, from 2019 to 2020. The results indicated that strains GX2019-010 to GX2019-013 and GX2019-015 to GX2019-018 were highly virulent, while strain GX2020-019 exhibited moderate virulence. Strain GX2019-014 was characterized as a wild-type strain with low virulence, displaying no pathogenic effects when 0.5 mL containing 106 TCID50 virus was inoculated into the muscle of specific pathogen-free (SPF) chickens at 4 weeks of age, while 107 TCID50 and 108 TCID50 resulted in mortality rates of 80 and 100%, respectively. The whole genomes of strains GX2019-010 to GX2019-013, GX2019-015 to GX2019-018, and GX2020-019 showed high homology with other Chinese newly emerging highly pathogenic FAdV-4 strains, whereas GX2019-014 was closer to nonmutant strains and shared the same residues with known nonpathogenic strains (B1-7, KR5, and ON1) at positions 219AA and 380AA of the Fiber-2 protein. Our work enriches the research on prevalent strains of FAdV-4 in China, expands the knowledge on the virulence diversity of the novel genotype FAdV-4, and provides valuable reference material for further investigations into the key virulence-associated genetic loci of FAdV-4

Chicken IFI6 inhibits avian reovirus replication and affects related innate immune signaling pathways

Interferon-alpha inducible protein 6 (IFI6) is an important interferon-stimulated gene. To date, research on IFI6 has mainly focused on human malignant tumors, virus-related diseases and autoimmune diseases. Previous studies have shown that IFI6 plays an important role in antiviral, antiapoptotic and tumor-promoting cellular functions, but few studies have focused on the structure or function of avian IFI6. Avian reovirus (ARV) is an important virus that can exert immunosuppressive effects on poultry. Preliminary studies have shown that IFI6 expression is upregulated in various tissues and organs of specific-pathogen-free chickens infected with ARV, suggesting that IFI6 plays an important role in ARV infection. To analyze the function of avian IFI6, particularly in ARV infection, the chicken IFI6 gene was cloned, a bioinformatics analysis was conducted, and the roles of IFI6 in ARV replication and the innate immune response were investigated after the overexpression or knockdown of IFI6 in vitro. The results indicated that the molecular weight of the chicken IFI6 protein was approximately 11 kDa and that its structure was similar to that of the human IFI27L1 protein. A phylogenetic tree analysis of the IFI6 amino acid sequence revealed that the evolution of mammals and birds was clearly divided into two branches. The evolutionary history and homology of chickens are similar to those of other birds. Avian IFI6 localized to the cytoplasm and was abundantly expressed in the chicken lung, intestine, pancreas, liver, spleen, glandular stomach, thymus, bursa of Fabricius and trachea. Further studies demonstrated that IFI6 overexpression in DF-1 cells inhibited ARV replication and that the inhibition of IFI6 expression promoted ARV replication. After ARV infection, IFI6 modulated the expression of various innate immunity-related factors. Notably, the expression patterns of MAVS and IFI6 were similar, and the expression patterns of IRF1 and IFN-β were opposite to those of IFI6. The results of this study further advance the research on avian IFI6 and provide a theoretical basis for further research on the role of IFI6 in avian virus infection and innate immunity

A Novel Metasurface Lens Design for Synthesizing Plane Waves in Millimeter-Wave Bands

With the development of communication technology has come several measurement applications requiring plane-wave conditions for wireless-device characterizations in anechoic chambers. In this paper, a metasurface lens with a 2 × 2 feeding-antenna array is proposed and characterized to synthesize a plane wave in a near field for a fifth-generation (5G) millimeter-wave radio-frequency (RF) devices test. The metasurface lens, based on Jerusalem-cross elements printed on a printed circuit board (PCB) substrate, is used for controlling the phase-shift distribution of incident spherical waves. The lens has a size of 0.4 × 0.4 m and is designed to operate at a range from 24.25 GHz to 27.5 GHz, and its feeding-antenna array is located at a focal plane of the lens, which is parallel to the metasurface lens. The lens is studied and verified through simulations and experiments, and a uniform amplitude and phase-field distribution at a reduced distance of 1.2 m generated by the metasurface lens throughout a QZ are achieved. The worst-case amplitude and phase variation of the designed metasurface lens are ±0.75 dB and ±7.5°, respectively. The results show a plane-wave condition can be achieved in 5G millimeter bands through the proposed compact and effective metasurface lens. Moreover, the proposed metasurface lens is shown to be capable of reducing the plane-wave synthesizing distance compared to the compact antenna test range (CATR) with a significantly reduced system cost, making it an attractive alternative to antenna testing in 5G millimeter-wave frequency bands

Aspirin and verapamil increase the sensitivity of Candida albicans to caspofungin under planktonic and biofilm conditions

Objectives: This study aimed to investigate the effects of caspofungin (CAS) combined with aspirin (ASP) or verapamil (VPL) on the sensitivity of Candida albicans under planktonic and biofilm conditions. Methods: A total of 39 C. albicans clinical strains were used to construct biofilms. Sensitivity to ASP or VPL combined with CAS was analysed by broth microdilution. MIC50 values were obtained and the fractional inhibitory concentration index (FICI) was calculated. Subsequently, C. albicans ZY22 was selected for time–growth curve analysis and strains ZY15 and ZY22 were used for time–kill curve analysis. Results: Under planktonic condition the MIC50 of CAS was 0.0313–8 μg/mL following treatment with CAS alone, whereas it decreased to 0.0313–4 μg/mL following CAS combined with ASP or VPL. Under biofilm condition the MIC50 of CAS was 0.125–16 μg/mL following treatment with CAS alone, whereas it decreased to 0.0625–16 μg/mL or 0.0625–8 μg/mL following CAS combined with ASP or VPL. FICI results showed synergistic interactions between CAS and ASP under planktonic and biofilm conditions in 17 and 16 strains, respectively. However, synergistic interactions between CAS and VPL under planktonic and biofilm conditions were observed in 19 and 23 strains, respectively. Additionally, 8000 μg/mL ASP or 8 μg/mL VPL combined with CAS had better inhibitory effects on C. albicans. Conclusion: ASP and VPL may be a sensitiser for CAS, and the antifungal effects of CAS may be sensitised by 8000 μg/mL ASP or 8 μg/mL VPL against C. albicans under planktonic and biofilm conditions

A Novel Metasurface Lens Design for Synthesizing Plane Waves in Millimeter-Wave Bands

With the development of communication technology has come several measurement applications requiring plane-wave conditions for wireless-device characterizations in anechoic chambers. In this paper, a metasurface lens with a 2 × 2 feeding-antenna array is proposed and characterized to synthesize a plane wave in a near field for a fifth-generation (5G) millimeter-wave radio-frequency (RF) devices test. The metasurface lens, based on Jerusalem-cross elements printed on a printed circuit board (PCB) substrate, is used for controlling the phase-shift distribution of incident spherical waves. The lens has a size of 0.4 × 0.4 m and is designed to operate at a range from 24.25 GHz to 27.5 GHz, and its feeding-antenna array is located at a focal plane of the lens, which is parallel to the metasurface lens. The lens is studied and verified through simulations and experiments, and a uniform amplitude and phase-field distribution at a reduced distance of 1.2 m generated by the metasurface lens throughout a QZ are achieved. The worst-case amplitude and phase variation of the designed metasurface lens are ±0.75 dB and ±7.5°, respectively. The results show a plane-wave condition can be achieved in 5G millimeter bands through the proposed compact and effective metasurface lens. Moreover, the proposed metasurface lens is shown to be capable of reducing the plane-wave synthesizing distance compared to the compact antenna test range (CATR) with a significantly reduced system cost, making it an attractive alternative to antenna testing in 5G millimeter-wave frequency bands

Long Noncoding RNA HOTAIR Controls Cell Cycle by Functioning as a Competing Endogenous RNA in Esophageal Squamous Cell Carcinoma

AbstractRecent studies have shown that long noncoding RNAs (lncRNAs) play pivotal roles in the initiation and progression of cancer, including esophageal squamous cell carcinoma (ESCC). The lncRNA HOX transcript antisense RNA (HOTAIR) was reported to be dysregulated and correlated with the progression of ESCC. However, the biological role and the underlying mechanism of HOTAIR in the development of ESCC remain unclear. Herein, we found that HOTAIR was aberrantly upregulated in ESCC cells and that HOTAIR depletion inhibited proliferation and led to G1 cell cycle arrest in ESCC cells. Besides, we found that HOTAIR acted as an endogenous sponge to downregulate miR-1 expression by directly binding to miR-1. Furthermore, HOTAIR overturned the effect of miR-1 on the proliferation and cell cycle profile in ESCC cells, which involved the derepression of cyclin D1 (CCND1) expression, a target of miR-1. Taken together, our study elucidated a novel HOTAIR /miR-1/CCND1 regulatory axis in which HOTAIR acted as a competing endogenous RNA by sponging miR-1 and upregulated CCND1 expression, thereby facilitating the tumorigenesis of ESCC. Investigation of this lncRNA/miRNA/mRNA pathway may contribute to a better understanding of ESCC pathogenesis and facilitate the development of lncRNA-directed therapy against this disease

Simultaneous Determination of Coumarin and Its Derivatives in Tobacco Products by Liquid Chromatography-Tandem Mass Spectrometry

In this paper an analytical method based on high performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) for the determination of coumarin and its derivatives in tobacco products was developed. The MS/MS fragmentation pathways of the eight coumarins were elucidated. The new analytical method was defined based on two main axes, an extraction procedure with acetonitrile and analyte detection performed by HPLC-MS/MS in electron impact mode. The excellent selectivity and sensitivity achieved in multiple reaction monitoring (MRM) mode allowed satisfactory confirmation and quantitation for the coumarin flavor additives. Under the optimized gradient elution conditions, it took only 4.5 min to separate all eight coumarins. Good linearity for all the analytes were confirmed by the correlation coefficient r2, ranging from 0.9987 to 0.9996. The limits of detection (LODs) and limits of quantitation (LOQs) of these compounds were in the range of 0.5–1.7 μg/kg and 1.7–5.2 μg/kg, respectively. The average recoveries at three spiked levels (LOQ, 1.5LOQ, 2LOQ) were all in the range of 69.6%–95.1% with RSDs (n = 6) lower than 5.3%. The method of HPLC-MS/MS developed in this study was initially applied to the research of coumarin flavor additives in tobacco products collected from the located market in Beijing from China and proved to be accurate, sensitive, convenient and practical