1,131 research outputs found
Planet–sun sensor revisited
Since the seminal work of Daniele Mortari (“Moon-Sun Attitude Sensor,” Journal of Spacecraft and Rockets, Vol. 34, No. 3, 1997, pp. 360–364), the concept of an attitude sensor using images of illuminated celestial bodies has been pushed forward through the years. The basic idea consists of extracting two independent directions from the image of a celestial body, namely, the camera-to-planet and the planet-to-sun directions. The former is estimated from the center of an ellipse fitted to the imaged limb points and the latter from the symmetry axis of the illuminated region. These assumptions, however, only hold for far-distant spherical targets. In this work, the problem is reformulated in the framework of projective camera transformations of quadrics and conics, and an algorithm estimating the line of sight to the planet and the illumination direction from the limb and terminator ellipses, respectively, is presented. The method is applicable to any ellipsoidlike celestial body having known orientation. The algorithm is first validated on synthetically generated images and then tested using real pictures of Dione and Enceladus satellites gathered from Cassini spacecraft. Results show that the sensor concept returns rms errors in the order of the angular width of a pixel in computing the nadir direction, and subdegree accuracy in computing the sun direction
Growth of Rhodococcus sp. strain BCP1 on gaseous n-alkanes: New metabolic insights and transcriptional analysis of two soluble di-iron monooxygenase genes
Rhodococcus sp. strain BCP1 was initially isolated for its ability to grow on gaseous n-alkanes, which act as inducers for the co-metabolic degradation of low-chlorinated compounds. Here, both molecular and metabolic features of BCP1 cells grown on gaseous and short-chain n-alkanes (up to n-heptane) were examined in detail. We show that propane metabolism generated terminal and sub-terminal oxidation products such as 1- and 2-propanol, whereas 1-butanol was the only terminal oxidation product detected from n-butane metabolism. Two gene clusters, prmABCD and smoABCD-coding for Soluble Di-Iron Monooxgenases (SDIMOs) involved in gaseous n-alkanes oxidation-were detected in the BCP1 genome. By means of Reverse Transcriptase-quantitative PCR (RT-qPCR) analysis, a set of substrates inducing the expression of the sdimo genes in BCP1 were assessed as well as their transcriptional repression in the presence of sugars, organic acids, or during the cell growth on rich medium (Luria-Bertani broth). The transcriptional start sites of both the sdimo gene clusters were identified by means of primer extension experiments. Finally, proteomic studies revealed changes in the protein pattern induced by growth on gaseous- (n-butane) and/or liquid (n-hexane) short-chain n-alkanes as compared to growth on succinate. Among the differently expressed protein spots, two chaperonins and an isocytrate lyase were identified along with oxidoreductases involved in oxidation reactions downstream of the initial monooxygenase reaction step
Rhodococcus aetherivorans BCP1 as cell factory for the production of intracellular tellurium nanorods under aerobic conditions
Background: Tellurite (TeO32-) is recognized as a toxic oxyanion to living organisms. However, mainly anaerobic or facultative-anaerobic microorganisms are able to tolerate and convert TeO32- into the less toxic and available form of elemental Tellurium (Te0), producing Te-deposits or Te-nanostructures. The use of TeO32--reducing bacteria can lead to the decontamination of polluted environments and the development of "green-synthesis" methods for the production of nanomaterials. In this study, the tolerance and the consumption of TeO32- have been investigated, along with the production and characterization of Te-nanorods by Rhodococcus aetherivorans BCP1 grown under aerobic conditions. Results: Aerobically grown BCP1 cells showed high tolerance towards TeO32- with a minimal inhibitory concentration (MIC) of 2800μg/mL (11.2mM). TeO32- consumption has been evaluated exposing the BCP1 strain to either 100 or 500μg/mL of K2TeO3 (unconditioned growth) or after re-inoculation in fresh medium with new addition of K2TeO3 (conditioned growth). A complete consumption of TeO32- at 100μg/mL was observed under both growth conditions, although conditioned cells showed higher consumption rate. Unconditioned and conditioned BCP1 cells partially consumed TeO32- at 500μg/mL. However, a greater TeO32- consumption was observed with conditioned cells. The production of intracellular, not aggregated and rod-shaped Te-nanostructures (TeNRs) was observed as a consequence of TeO32- reduction. Extracted TeNRs appear to be embedded in an organic surrounding material, as suggested by the chemical-physical characterization. Moreover, we observed longer TeNRs depending on either the concentration of precursor (100 or 500μg/mL of K2TeO3) or the growth conditions (unconditioned or conditioned grown cells). Conclusions:Rhodococcus aetherivorans BCP1 is able to tolerate high concentrations of TeO32- during its growth under aerobic conditions. Moreover, compared to unconditioned BCP1 cells, TeO32- conditioned cells showed a higher oxyanion consumption rate (for 100μg/mL of K2TeO3) or to consume greater amount of TeO32- (for 500μg/mL of K2TeO3). TeO32- consumption by BCP1 cells led to the production of intracellular and not aggregated TeNRs embedded in an organic surrounding material. The high resistance of BCP1 to TeO32- along with its ability to produce Te-nanostructures supports the application of this microorganism as a possible eco-friendly nanofactory
The Use of Fura-2 Fluorescence to Monitor the Movement of Free Calcium Ions into the Matrix of Plant Mitochondria (Pisum sativum and Helianthus tuberosus)
A haptic-enabled multimodal interface for the planning of hip arthroplasty
Multimodal environments help fuse a diverse range of sensory modalities, which is particularly important when integrating the complex data involved in surgical preoperative planning. The authors apply a multimodal interface for preoperative planning of hip arthroplasty with a user interface that integrates immersive stereo displays and haptic modalities. This article overviews this multimodal application framework and discusses the benefits of incorporating the haptic modality in this area
Assembly, growth and conductive properties of tellurium nanorods produced by Rhodococcus aetherivorans BCP1
Tellurite (TeO32-) is a hazardous and toxic oxyanion for living organisms. However, several microorganisms can bioconvert TeO32- into the less toxic form of elemental tellurium (Te0). Here, Rhodococcus aetherivorans BCP1 resting (non-growing) cells showed the proficiency to produce tellurium-based nanoparticles (NPs) and nanorods (NRs) through the bioconversion of TeO32-, depending on the oxyanion initial concentration and time of cellular incubation. Te-nanostructures initially appeared in the cytoplasm of BCP1 cells as spherical NPs, which, as the exposure time increased, were converted into NRs. This observation suggested the existence of an intracellular mechanism of TeNRs assembly and growth that resembled the chemical surfactant-assisted process for NRs synthesis. The TeNRs produced by the BCP1 strain showed an average length (>700 nm) almost doubled compared to those observed in other studies. Further, the biogenic TeNRs displayed a regular single-crystalline structure typically obtained for those chemically synthesized. The chemical-physical characterization of the biogenic TeNRs reflected their thermodynamic stability that is likely derived from amphiphilic biomolecules present in the organic layer surrounding the NRs. Finally, the biogenic TeNRs extract showed good electrical conductivity. Thus, these findings support the suitability of this strain as eco-friendly biocatalyst to produce high quality tellurium-based nanomaterials exploitable for technological purposes
Stabilized hot electron bolometer heterodyne receiver at 2.5 THz
We report on a method to stabilize a hot electron bolometer (HEB) mixer at 2.5 THz. The technique utilizes feedback control of the local oscillator (LO) laser power by means of a swing-arm actuator placed in the optical beam path. We demonstrate that this technique yields a factor of 50 improvement in the spectroscopic Allan variance time which is shown to be over 30 s in a 12 MHz noise fluctuation bandwidth. Furthermore, broadband signal direct detection effects may be minimized by this technique. The technique is versatile and can be applied to practically any local oscillator at any frequency
TRIS II: search for CMB spectral distortions at 0.60, 0.82 and 2.5 GHz
With the TRIS experiment we have performed absolute measurements of the sky
brightness in a sky circle at at the frequencies
0.60, 0.82 and 2.5 GHz. In this paper we discuss the techniques used to
separate the different contributions to the sky emission and give an evaluation
of the absolute temperature of the Cosmic Microwave Background. For the
black-body temperature of the CMB we get: at GHz; at GHz; at
GHz. The first error bar is statistic (1) while the second
one is systematic. These results represent a significant improvement with
respect to the previous measurements. We have also set new limits to the
free-free distortions, ,
and slightly improved the Bose-Einstein upper limit, , both at 95% confidence level.Comment: accepted for publication in The Astrophysical Journa
How glassy are orientational dynamics of rodlike molecules near the isotropic-nematic transition?
In an attempt to quantitatively characterize the recently observed slow
dynamics in the isotropic and nematic phase of liquid crystals, we investigate
the single-particle orientational dynamics of rodlike molecules across the
isotropic-nematic transition in computer simulations of a family of model
systems of thermotropic liquid crystals. Several remarkable features of glassy
dynamics are on display including non-exponential relaxation, dynamical
heterogeneity, and non-Arrhenius temperature dependence of the orientational
relaxation time. In order to obtain a quantitative measure of glassy dynamics
in line with the estbalished methods in supercooled liquids, we construct a
relaxation time versus scaled inverse temperature plot, and demonstrate that
one can indeed define a 'fragility index' for thermotropic liquid crystals,
that depends on density and aspect ratio. The values of the fragility parameter
are surprisingly in the range one observed for glass forming liquids. A
plausible correlation between the energy landscape features and the observed
fragility is discussed.Comment: 7 figures and 8 page
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