Cervix dilation and transcervical embryo recovery in cervical Santa Inês sheep.

Proceedings of the 28th Annual Meeting of the Brazilian Embryo Technology Society (SBTE), August, 2014, Natal, RN, Brazil. Abstracts

Embryo production and recovery in goats by non-surgical transcervical technique.

AbstractThe objective of this study was to evaluate the efficiency of non-surgical embryo collection in goats. Five lactating Saanen goats were tested alternately in a cross-over design for two treatments (T1 and T2). In both treatments, intravaginal progesterone devices were inserted (day 0/6p.m.) and removed six days later (day 6/18:00p.m.) and 22.5μg cloprostenol were administered by intravulvosubmucosal via (day 0). From day 4 (18:00 p.m.), goats received six decreasing doses of FSH (250–400IU) at 12h interval. Three doses of 50mg flunixin meglumine were administered on days 9, 10, and 11. In T1 and T2, animals received two doses of saline or 250mg of rbST on day 0 and on first detection of estrus, respectively. Goats were mated until the end of estrus and uteri were flushed by cervical route seven days after first mating. The embryo collection interval was 60days. Percentage of flushing medium recovered was close to 100% in both treatments. Total number of ova (18.4±9.4 and 15.7±11.0) and viable embryos (13.4±4.1 and 8.2±3.0) were similar (P>0.05) for T1 and T2, respectively. Short-term progesterone protocol was efficient for superovulation in lactating Saanen goats but association with rbST did not enhance embryo recovery or quality. Non surgical flushing by cervical via was efficient to recover flushing medium and embryos in goats