A binary mixture of spinor atomic Bose-Einstein condensates

We study the ground state and classify its phase diagram for a mixture of two spin-1 condensates in the absence of external magnetic (B-) field according to atomic parameters for intra- and inter-species spin exchange coupling and singlet pairing interaction. Ignoring the inter-species singlet pairing interaction, the ground state phases are found analytically. Numerical approach of simulated annealing is adopted when the singlet pairing interaction is present. Our results on the phase diagram and the boundaries between phases allow for easy identifications of quantum phase transitions, that can be induced through the tuning of optical traps and atom numbers. They provide the first insight and guidance for several ongoing experiments on mixtures of spinor condensates.Comment: 5 pages, 4 figure

Quantum spin mixing in a binary mixture of spin-1 atomic condensates

We study quantum spin mixing in a binary mixture of spin-1 condensates including coherent interspecies mixing process, using the familiar spinor condensates of $^{87}$Rb and $^{23}$Na atoms in the ground lower hyperfine F=1 manifolds as prototype examples. Within the single spatial mode approximation for each of the two spinor condensates, the mixing dynamics reduce to that of three coupled nonlinear pendulums with clear physical interpretations. Using suitably prepared initial states, it is possible to determine the interspecies singlet-pairing as well as spin-exchange interactions from the subsequent mixing dynamics.Comment: 6 pages, 3 figure

Searching for high-KK isomers in the proton-rich A∼80A\sim80 mass region

Configuration-constrained potential-energy-surface calculations have been performed to investigate the $K$ isomerism in the proton-rich $A\sim80$ mass region. An abundance of high-$K$ states are predicted. These high-$K$ states arise from two and four-quasi-particle excitations, with $K^{\pi}=8^{+}$ and $K^{\pi}=16^{+}$, respectively. Their excitation energies are comparatively low, making them good candidates for long-lived isomers. Since most nuclei under studies are prolate spheroids in their ground states, the oblate shapes of the predicted high-$K$ states may indicate a combination of $K$ isomerism and shape isomerism

Quantum states of a binary mixture of spinor Bose-Einstein condensates

We study the structure of quantum states for a binary mixture of spin-1 atomic Bose-Einstein condensates. In contrast to collision between identical bosons, the s-wave scattering channel between inter-species does not conform to a fixed symmetry. The spin-dependent Hamiltonian thus contains non-commuting terms, making the exact eigenstates more challenging to obtain because they now depend more generally on both the intra- and inter-species interactions. We discuss two limiting cases, where the spin-dependent Hamiltonian reduces respectively to sums of commuting operators. All eigenstates can then be directly constructed, and they are independent of the detailed interaction parameters.Comment: 5 pages, no figure

Vacuolar ATPase depletion contributes to dysregulation of endocytosis in bloodstream forms of Trypanosoma brucei

BACKGROUND Vacuolar H-ATPase (V-ATPase) is a highly conserved protein complex which hydrolyzes ATP and pumps protons to acidify vacuolar vesicles. Beyond its role in pH maintenance, the involvement of V-ATPase in endocytosis is well documented in mammals and plants but is less clear in Trypanosoma brucei. METHODS In this study, the subcellular localization of V-ATPase subunit B (TbVAB) of T. brucei was assessed via in situ N-terminal YFP-tagging and immunofluorescence assays. Transgenic bloodstream forms (BSF) of T. brucei were generated which comprised either a V-ATPase subunit B (TbVAB) conditional knockout or a V-ATPase subunit A (TbVAA) knockdown. Acridine orange and BCECF-AM were employed to assess the roles of V-ATPase in the pH regulation of BSF T. brucei. The endocytic activities of three markers were also characterized by flow cytometry analyses. Furthermore, trypanosomes were counted from trypanolysis treatment groups (either containing 1% or 5% NHS) and endocytosed trypanosome lytic factor (TLF) was also analyzed by an immunoblotting assay. RESULTS TbVAB was found to localize to acidocalcisomes, lysosomes and probably also to endosomes of BSF of T. brucei and was demonstrated to be essential for cell growth. TbVAB depletion neutralized acidic organelles at 24 hours post-tetracycline depletion (hpd), meanwhile the steady state intracellular pH increased from 7.016 ± 0.013 to 7.422 ± 0.058. Trypanosomes with TbVAB depletion at 24 hpd were found to take up more transferrin (2.068 ± 0.277 fold) but less tomato lectin (49.31 ± 22.57%) by endocytosis, while no significant change was detected in dextran uptake. Similar endocytic dysregulated phenotypes were also observed in TbVAA knockdown cells. In addition, TbVAB depleted trypanosomes showed a low uptake of TLF and exhibited less sensitive to lysis in both 1% and 5% NHS treatments. CONCLUSIONS TbVAB is a key component of V-ATPase and was found to play a key function in endocytosis as well as exhibiting different effects in a receptor/cargo dependent manner in BSF of T. brucei. Besides vacuolar alkalinization, the dysregulation of endocytosis in TbVAB depleted T. brucei is considered to contribute to the reduced sensitivity to lysis by normal human serum