Antiviral treatment alters the frequency of activating and inhibitory receptor-expressing natural killer cells in chronic Hepatitis B virus infected patients

Natural killer (NK) cells play a critical role in innate antiviral immunity, but little is known about the impact of antiviral therapy on the frequency of NK cell subsets. To this aim, we performed this longitudinal study to examine the dynamic changes of the frequency of different subsets of NK cells in CHB patients after initiation of tenofovir or adefovir therapy. We found that NK cell numbers and subset distribution differ between CHB patients and normal subjects; furthermore, the association was found between ALT level and CD158b+ NK cell in HBV patients. In tenofovir group, the frequency of NK cells increased during the treatment accompanied by downregulated expression of NKG2A and KIR2DL3. In adefovir group, NK cell numbers did not differ during the treatment, but also accompanied by downregulated expression of NKG2A and KIR2DL3. Our results demonstrate that treatment with tenofovir leads to viral load reduction, and correlated with NK cell frequencies in peripheral blood of chronic hepatitis B virus infection. In addition, treatments with both tenofovir and adefovir in chronic HBV infected patients induce a decrease of the frequency of inhibitory receptor+ NK cells, which may account for the partial restoration of the function of NK cells in peripheral blood following treatment

Development of a multi-container extrusion method for extruding lightweight wide plates and sheets

Extrusion of wide plates and sheets of light alloys has been studied over a long period of time, yet the extrudable width of the material is still limited due to high extrusion force requirement. To overcome this drawback, a new multi-container extrusion process is proposed in the research, which allows the production of lightweight plates and sheets with less force compared to that of existing extrusion methods. A lab scale feasibility study system with three containers has been designed and built and tested for AA1060 billets. Experimental work has been carried out with the extrusion temperature of 450°C and extrusion speed of 0.5 mm/s. Optical microscopy observation and tensile tests have been performed for the extruded materials at different positions to investigate the extrusion welding quality between the three extrusion billets. The test results show that the welding quality improves as extrusion progresses and the overall welding quality is stable. This study demonstrates the feasibility of the new multi-container extrusion method

Effect of montmorillonite on arsenic accumulation in common carp

The effect of montmorillonite (MMT) on dietary arsenic (As(III)) accumulation in tissues of common carp was investigated. Growth rates and survival do not appear to be sensitive indicators of dietary As(III) toxicity under lower exposure concentration. However, the toxicity increases as As(III) exposure concentration increase, and this can be alleviated with added MMT concentration. Exposure of common carp to dietary As(III) resulted in a significant As(III) accumulation in the tissues of common carp in the order: intestine>bone>gill>liver>muscle>brain (P<0.05), indicating accumulation of these tissues. When fed with MMT, there was a reduction in As(III) concentration of these tissues compared with the control group. The ratio of oxidized glutathione to reduced glutathione (GSSG/GSH) revealed a significant effect with MMT concentration (1.0%) (p<0.05) and post-hoc analysis also revealed that the group treated with MMT exhibited a decrease in the ratio of GSSG/GSH when compared to the fish not treated with MMT. Moreover, the addition of MMT to the diet with As(III) decreased metallothionein concentration. The added dietary MMT was therefore clearly protective against the bioaccumulation of As(III).Key words: Montmorillonite, arsenic, common carp

Expression of polyhedrin-hEGF fusion protein in cultured cells and larvae of Bombyx mori

For mass production of human epidermal growth factor (hEGF), silkworm baculovirus expression vector system (BEVS) was adopted in this study. hEGF gene was in-frame fused with polyhedrin (Ph) gene under the control of Ph promoter and was used to co-transfect BmN cell with the modifiedBombyx mori baculovirus DNA to obtained recombinant virus. The ELISA showed a maximum expression on day 4 in larvae and pupae. Both cellular extracts and haemolymph of silkworm larvae infected with rBacPh-EGF could all support the proliferation of Balb/c 3T3 cell. The correspondingmaterials from BmN cell and silkworm larvae infected with wild virus also indicated a weak effect of upregulation on 3T3 cells proliferation. The animal study showed that both pupae infected with rBacPh-EGF virus and wild virus protected the gastric mucosa against ethanol-induced damage in rats,although the protection from pupae infected with wild virus was slightly weak. The mechanism is under investigatin

Pressure shift of the superconducting T_c of LiFeAs

The effect of hydrostatic pressure on the superconductivity in LiFeAs is investigated up to 1.8 GPa. The superconducting transition temperature, T_c, decreases linearly with pressure at a rate of 1.5 K/GPa. The negative pressure coefficient of T_c and the high ambient pressure T_c indicate that LiFeAs is the high-pressure analogue of the isoelectronic SrFe_2As_2 and BaFe_2As_2.Comment: 3 pages, 2 figure

A new 111 type iron pnictide superconductor LiFeP

A new iron pnictide LiFeP superconductor was found. The compound crystallizes into a Cu2Sb structure containing an FeP layer showing superconductivity with maximum Tc of 6K. This is the first 111 type iron pnictide superconductor containing no arsenic. The new superconductor is featured with itinerant behavior at normal state that could helpful to understand the novel superconducting mechanism of iron pnictide compounds.Comment: 3 figures + 1 tabl

Diversity of eukaryotic plankton of aquaculture ponds with Carassius auratus gibelio, using denaturing gradient gel electrophoresis

PCR-denaturing gradient gel electrophoresis (DGGE) and canonical correspondence analysis (CCA) were used to explore the relationship between eukaryotic plankton community succession and environmental factors in two aquaculture pond models with gibel carp Carassius auratus gibelio. The main culture species of pond 1 were gibel carp and grass carp, and the combined density was 46224 fingerling/ha (gibel carp/grass carp/silver carp/bighead carp, 17:4:6:1). The main culture species of pond 2 was gibel carp, and the combined density was 37551 fingerling/ha (gibel carp/silver carp/bighead carp, 52:1:1). Water samples were collected monthly. The results showed that the annual average concentrations of TP and PO_4-P in pond 1 were significantly higher than pond 2 (p>0.05). The concentration of chlorophyll a (chl a) has no significantly difference between pond 1 and pond 2. DGGE profiles of 18S rRNA gene fragments from the two ponds revealed that the diversity of eukaryotic plankton assemblages was highly variable. 91 bands and 71 bands were detected in pond 1 and pond 2, respectively. The average Shannon–Wiener index of pond 1 was significantly higher than pond 2. Canonical correspondence analysis (CCA) revealed that temperature played a key role in the structure of the eukaryotic plankton community in both ponds, but the nutrient concentration did not affect it. Our results suggest that DGGE method is a cost-effective way to gain insight into seasonal dynamics of eukaryotic plankton communities in culture ponds, and the increase in the number of filter-feeding silver carp and bighead carp could increase the diversity of the eukaryotic plankton community

Momentum-Resolved Electronic Structure of the High-TcT_{c} Superconductor Parent Compound BaBiO3_{3}

We investigate the band structure of BaBiO$_{3}$, an insulating parent compound of doped high-$T_{c}$ superconductors, using \emph{in situ} angle-resolved photoemission spectroscopy on thin films. The data compare favorably overall with density functional theory calculations within the local density approximation, demonstrating that electron correlations are weak. The bands exhibit Brillouin zone folding consistent with known BiO$_{6}$ breathing distortions. Though the distortions are often thought to coincide with Bi$^{3+}$/Bi$^{5+}$ charge ordering, core level spectra show that bismuth is monovalent. We further demonstrate that the bands closest to the Fermi level are primarily oxygen derived, while the bismuth $6s$ states mostly contribute to dispersive bands at deeper binding energy. The results support a model of Bi-O charge transfer in which hole pairs are localized on combinations of the O $2p$ orbitals.Comment: minor changes to text and other figures; includes link to online Supplemental Material; accepted to Phys. Rev. Let