Screening of Satureja subspicata Vis. honey by HPLC-DAD, GC-FID/MS and UV/VIS: prephenate derivatives as biomarkers

The samples of Satureja subspicata Vis. honey were confirmed to be unifloral by melissopalynological analysis with the characteristic pollen share from 36% to 71%. Bioprospecting of the samples was performed by HPLC-DAD, GC-FID/MS, and UV/VIS. Prephenate derivatives were shown to be dominant by the HPLC-DAD analysis, particularly phenylalanine (167.8 mg/kg) and methyl syringate (MSYR, 114.1 mg/kg), followed by tyrosine and benzoic acid. Higher amounts of MSYR (3-4 times) can be pointed out for distinguishing S. subspicata Vis. honey from other Satureja spp. honey types. GC-FID/MS analysis of ultrasonic solvent extracts of the samples revealed MSYR (46.68%, solvent pentane/Et2O 1:2 (v/v); 52.98%, solvent CH2Cl2) and minor abundance of other volatile prephenate derivatives, as well as higher aliphatic compounds characteristic of the comb environment. Two combined extracts (according to the solvents) of all samples were evaluated for their antioxidant properties by FRAP and DPPH assay; the combined extracts demonstrated higher activity (at lower concentrations) in comparison with the average honey sample. UV/VIS analysis of the samples was applied for determination of CIE Lab colour coordinates, total phenolics (425.38 mg GAE/kg), and antioxidant properties (4.26 mmol Fe2+/kg (FRAP assay) and 0.8 mmol TEAC/kg (DDPH assay)

Radon Equilibrium Factor in Selected Dwellings and Workplaces in Montenegro and Serbia

U radu su prikazani rezultati merenja radonskog ravnotežnog faktora aktivnom metodom na 5 lokacija u Crnoj Gori i Srbiji. Dobijeni rezultati su u rasponu od 0.36 do 0.7, što je u proseku veće od preporučene vrednosti od 0.4. Na istim lokacijama su izvršena interkomparativna merenja koncentracije radona (uređajima Alpha Guard Pro, RAD7 i RTM1688-2), ravnotežne ekvivalentne koncentracije radona (uređajima RNWL i RPM2200) i radonskog ravnotežnog faktora. Slaganje rezultata je testirano Z-testom koji ukazuje da ne postoji statistički značajno odstupanje između rezultata dobijenih različitim mernim uređajima, odnosno ne postoji nekonzistentnost između različitih metodologija merenja kao ni potrebe za harmonizacijom različitih procedura merenja.In this contribution, the results of radon equilibrium factor measurement in selected dwelling and workplaces in Montenegro and Serbia are presented. Radon equilibrium factor was derived from measurements of indoor radon concentration and equilibrium equivalent radon concentration. Measurements were performed one day for each of 5 locations by using different active devices. Obtained radon equilibrium factors range from 0.36 to 0.7 which is in average larger than worldwide average of 0.4 Results are not surprising since measuring locations were kept closed during the measuring time, reducing therefore ventilation rate which as a consequence has an increase of equilibrium factor. Individual measuring results of indoor radon concentrations, equilibrium equivalent radon concentrations and radon equilibrium factors obtained with different detectors systems from 2 research teams was tested using Z – test. Obtained Z – score for each measurement vas below critical value, indication that there is no statistical difference between different measuring devices.XXXI Симпозијум Друштва за заштиту од зрачења Србије и Црне Горе, 06-08. октобар 2021.Proceedings: [https://vinar.vin.bg.ac.rs/handle/123456789/9668