11 research outputs found
School Climate and Adolescents’ Cyberbullying Perpetration: A Moderated Mediation Model of Moral Disengagement and Friends’ Moral Identity
Neuroprotective Effects of Phenylethanoid Glycosides from Cistanches salsa against 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-Induced Dopaminergic Toxicity in C57 Mice
Assimilating surface observations in a four-dimensional variational Doppler radar data assimilation system to improve the analysis and forecast of a squall line case
A novel PMA/PEG-based composite polymer electrolyte for all-solid-state sodium ion batteries
A Population-Based Acute Meningitis and Encephalitis Syndromes Surveillance in Guangxi, China, May 2007- June 2012
Luminescent and Magnetic Properties in Semiconductor Nanocrystals with Radial-Position-Controlled Mn 2+
Human hematopoietic stem/progenitor cells modified by zinc-finger nucleases targeted to CCR5 control HIV-1 in vivo
CCR5 is the major HIV-1 co-receptor, and individuals homozygous for a 32-bp deletion in CCR5 are resistant to infection by CCR5-tropic HIV-1. Using engineered zinc-finger nucleases (ZFNs), we disrupted CCR5 in human CD34(+) hematopoietic stem/progenitor cells (HSPCs) at a mean frequency of 17% of the total alleles in a population. This procedure produces both mono- and bi-allelically disrupted cells. ZFN-treated HSPCs retained the ability to engraft NOD/SCID/IL2rgamma(null) mice and gave rise to polyclonal multi-lineage progeny in which CCR5 was permanently disrupted. Control mice receiving untreated HSPCs and challenged with CCR5-tropic HIV-1 showed profound CD4(+) T-cell loss. In contrast, mice transplanted with ZFN-modified HSPCs underwent rapid selection for CCR5(-/-) cells, had significantly lower HIV-1 levels and preserved human cells throughout their tissues. The demonstration that a minority of CCR5(-/-) HSPCs can populate an infected animal with HIV-1-resistant, CCR5(-/-) progeny supports the use of ZFN-modified autologous hematopoietic stem cells as a clinical approach to treating HIV-1