Analysis of Strain State and Cracking of Cocnrete Sleepers

Prestressed concrete sleepers are the most common type of the sleepers used on the railroad. They serve as rail supports and absorb loads induced by trains. Sleepers are important for the durability and safety of the railroad and are exposed to various loads and an agressive environment during exploitation. Therefore, different types of appearing damage can determine their reliability. The article briefly discusses possible causes of damage and the deterioration of prestressed concrete sleepers. End cracking and damage at the rail seat was determined during the inspection of used sleepers. Therefore, the strain state and cracking of the rail seat of the sleeper was analysed under static and dynamic loads. The paper provides the obtained results of experimental research of used and new sleepers

siRNA–Mediated Methylation of Arabidopsis Telomeres

Chromosome termini form a specialized type of heterochromatin that is important for chromosome stability. The recent discovery of telomeric RNA transcripts in yeast and vertebrates raised the question of whether RNA–based mechanisms are involved in the formation of telomeric heterochromatin. In this study, we performed detailed analysis of chromatin structure and RNA transcription at chromosome termini in Arabidopsis. Arabidopsis telomeres display features of intermediate heterochromatin that does not extensively spread to subtelomeric regions which encode transcriptionally active genes. We also found telomeric repeat–containing transcripts arising from telomeres and centromeric loci, a portion of which are processed into small interfering RNAs. These telomeric siRNAs contribute to the maintenance of telomeric chromatin through promoting methylation of asymmetric cytosines in telomeric (CCCTAAA)n repeats. The formation of telomeric siRNAs and methylation of telomeres relies on the RNA–dependent DNA methylation pathway. The loss of telomeric DNA methylation in rdr2 mutants is accompanied by only a modest effect on histone heterochromatic marks, indicating that maintenance of telomeric heterochromatin in Arabidopsis is reinforced by several independent mechanisms. In conclusion, this study provides evidence for an siRNA–directed mechanism of chromatin maintenance at telomeres in Arabidopsis

Control of electroless nickel plating baths

Translated from Czech. (Koroze Ochr. Mater. 1986 (5) p. 102-104)Available from British Library Document Supply Centre- DSC:9022.06(BISI-Trans--26902)T / BLDSC - British Library Document Supply CentreSIGLEGBUnited Kingdo