Non-structural carbohydrate content in cryptogamic Antarctic species after two years of passive warming on the Fildes Peninsula

Cryptogamic vegetation dominates the ice-free areas of the maritime Antarctic. This particular flora grows slowly due to extreme environmental conditions, typically low temperature that may limit growth during a short summer. Over the last 50 years, the Antarctic Peninsula has undergone the highest registered temperature increases in Antarctica. As a consequence of higher temperatures, we hypothesized that lichens and mosses would produce more carbohydrates. To test this, open top chambers (OTCs) were installed in an Usnea-Himantormia community on Fildes Peninsula of King George Island. After two years, lichen thalli and plant tissues were collected to quantify non-structural carbohydrates in three lichens and two mosses. Responses contrasted between species. While non-structural carbohydrates were higher in the OTC for the lichen Himantormia lugubris, the values decreased in the moss Polytrichastrum alpinum. No marked responses to experimental warming were observed in the other three species. A significant species-specific increase in soluble sugar was observed inside the OTCs, while polyols content were not markedly different due to OTC treatment. In general, the obtained results indicate that warming does not increase carbohydrate content in all cryptogams, instead suggesting a strong species-specific response to a scenario of global warming. Further long-term warming experiments are needed to assess the responses of target species in the terrestrial Antarctic ecosystem

In Vitro Cultivars of Vaccinium corymbosum L. (Ericaceae) are a Source of Antioxidant Phenolics

The antioxidant activity and phenolic composition of six in vitro cultured blueberry seedlings were determined. Extracts were prepared in 85% ethanol from 30 days old in vitro cultured plants and used to evaluate the antioxidant capacities that included Ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picrylhydrazin (DPPH•) scavenging ability, total polyphenols (TP) and the partial phenolic composition performed by high performance liquid chromatography with diode array detector (HPLC-DAD), liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS (ESI-QqQ)). All ethanolic extracts from in vitro blueberry cultivars displayed antioxidant activity, with Legacy, Elliott and Bluegold cultivars being the most active. In addition, we observed a positive correlation between phenolic content and antioxidant activity. Our results suggest that the antioxidant activity of the extracts is related to the content of chlorogenic acid myricetin, syringic acid and rutin, and tissue culture of blueberry seedlings is a good tool to obtain antioxidant extracts with reproducible profile of compounds