Pulsed Interleaved MINFLUX

We introduce p-MINFLUX, a new implementation of the highly photon-efficient single-molecule localization method with a simplified experimental setup and additional fluorescence lifetime information. In contrast to the original MINFLUX implementation, p-MINFLUX uses interleaved laser pulses to deliver the doughnut-shaped excitation foci at a maximum repetition rate. Using both static and dynamic DNA origami model systems, we demonstrate the performance of p-MINFLUX for single-molecule localization nanoscopy and tracking, respectively. p-MINFLUX delivers 1-2 nm localization precision with 2000-1000 photon counts. In addition, p-MINFLUX gives access to the fluorescence lifetime enabling multiplexing and super-resolved lifetime imaging. p-MINFLUX should help to unlock the full potential of innovative single-molecule localization schemes.Fil: Masullo, Luciano Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; ArgentinaFil: Steiner, Florian. Ludwig Maximilians Universitat; AlemaniaFil: Zähringer, Jonas. Ludwig Maximilians Universitat; AlemaniaFil: Lopez, Lucía Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; ArgentinaFil: Bohlen, Johann. Ludwig Maximilians Universitat; AlemaniaFil: Richter, Lars. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; ArgentinaFil: Cole, Fiona. Ludwig Maximilians Universitat; AlemaniaFil: Tinnefeld, Philip. Ludwig Maximilians Universitat; AlemaniaFil: Stefani, Fernando Daniel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; Argentin

Ekoplasma - The Future of Complex Plasma Research in aboard the International Space Station

Ekoplasma is a joint German-Russian project, developing the future multi-purpose laboratory for the investigation of complex plasmas under microgravity conditions aboard the International Space Station (ISS). Complex plasmas are low-temperature plasmas, consisting of neutral gas atoms, ions, electrons and micro-meter sized particles as an additional component. The particles become charged in the plasma and as a result of their mutual repulsion form an optically thin cloud that can be studied in its full spatial and dynamical complexity on the granularity scale of each particle by optical cameras. Therefore, complex plasmas allow fundamental investigations down to the kinetic level of individual particles also for a wide field of interdisciplinary topics in classical condensed matter physics. Since gravity prevents the formation of large, homogeneous systems on earth, research on the ISS is essential, and Ekoplasma will follow in a line of successful preceding experiments aboard the ISS: PKE-Nefedov, PK-3 Plus and the currently operating PK-4 facility. Ekoplasma is planned to be launched to the ISS in 2022, and it will cover a wide range of research topics such as solidification and melting, phase separation in binary systems, the transition to turbulence, active matter or electrorheology

Pulsed RF-Discharge in Zyflex-Chamber

Interrupting rf-discharge is a well known method to control processes in plasmas. For example in semiconductor processing, it can be used to control growth of particles from material out of the plasma phase. By switching of the discharge, grown "fog" can be driven out of the plasma by gravity. [2] On the other hand, pulsing the plasma gives a good possibility to tune effective electron temperature independently from plasma density. This way you could tune parameters in complex plasma systems like electron density, electron temperature, screening length and particle charge in a more flexible way. Ekoplasma is a project designing the future lab for complex plasma research on the inter- national space station ISS. Together with its large cylindrical chamber Zyflex it will have many features to cover a wide range of physics in complex plasma research, now to be tested in lab and on parabolic flights. It also includes a multifunctional rf-generator which can be pulsed at different frequencies with on/off times even down to 50 µs. Combining this switching features with micro gravity conditions it will be possible to tune plasma parameters without loosing particles due to gravity pushing the particles. In this contribution, we show the plasma pulsing feature of the current lab setup of EkoPlasma and some first results from lab experiments

Pulsed rf-Discharge in Zyflex-Chamber

Interrupting rf-discharge is a well known method to control processes in plasmas. For example, in semiconductor processing it can be used to control growth of particles from material out of the plasma phase. By switching off the discharge, ”fog” of grown particles can be driven out of the plasma by gravity. On the other hand, pulsing the plasma gives a good possibility to tune effective electron temperature independently from plasma density. So you could tune interactions in complex plasma systems in a more flexible way. Ekoplasma will provide Plasmalab, the future lab for complex plasma research on the international space station ISS. Together with its large cylindrical chamber Zyflex it will have many features to cover a wide range of physics in complex plasma research. It also includes a multifunctional rf-generator which can be pulsed at different frequencies with on/off times even less than 50 mus. Combining the feature of switching the discharge on and off with micro gravity conditions it will be possible to tune plasma parameters without losing particles due to gravitational forces. In this contribution, we will show frst results of particles levitated in a pulsed gas discharge in the current lab setup of Ekoplasma