The multifunctional FUS, EWS and TAF15 proto-oncoproteins show cell type-specific expression patterns and involvement in cell spreading and stress response-5

G FET expression against beta actin expression in each sample and by further comparing RA treated with untreated cells at each time point. Data from one of two independent experiments yielding similar results.<p><b>Copyright information:</b></p><p>Taken from "The multifunctional FUS, EWS and TAF15 proto-oncoproteins show cell type-specific expression patterns and involvement in cell spreading and stress response"</p><p>http://www.biomedcentral.com/1471-2121/9/37</p><p>BMC Cell Biology 2008;9():37-37.</p><p>Published online 11 Jul 2008</p><p>PMCID:PMC2478660.</p><p></p

The multifunctional FUS, EWS and TAF15 proto-oncoproteins show cell type-specific expression patterns and involvement in cell spreading and stress response-2

Mage of each column Overexpression of FET-GFP proteins cause stress granule formation in transiently transfected HT-1080 cells. HT-1080 cells stably expressing FET-GFP proteins show localization of tagged proteins to stress granules upon arsenite treatment. Endogenous FET proteins localize to stress granules in response to oxidative stress in HeLa cells. Stable transfectants of FUSA-GFP and GFP show similar minor signal in granules upon arsenite treatment. Scale bars indicate 5 μm.<p><b>Copyright information:</b></p><p>Taken from "The multifunctional FUS, EWS and TAF15 proto-oncoproteins show cell type-specific expression patterns and involvement in cell spreading and stress response"</p><p>http://www.biomedcentral.com/1471-2121/9/37</p><p>BMC Cell Biology 2008;9():37-37.</p><p>Published online 11 Jul 2008</p><p>PMCID:PMC2478660.</p><p></p

The multifunctional FUS, EWS and TAF15 proto-oncoproteins show cell type-specific expression patterns and involvement in cell spreading and stress response-1

Y in nuclei. Scale bars indicate 10 μm. HT-1080 cells stably expressing FET-GFP proteins show nuclear localization of all three proteins and in addition cytoplasmic localization for FUS-GFP and TAF15-GFP. Scale bars indicate 10 μm. Western blots showing FET-GFP proteins of expected sizes and specificity of FET antibodies used. Wells contain the following lysates: FUS-GFP clone 1 (1), EWS-GFP clone 1 (2), TAF15-GFP clone 1 (3), GFP clone 1 (4), HT-1080 (5), FUS-GFP clone 2 (6), EWS-GFP clone 2 (7), TAF15-GFP clone 2 (8), GFP clone 2 (9). No crossreactivity is seen between different FET antibodies. Endogenous FET proteins correspond to the lower bands seen in all lanes and tagged FET proteins to upper bands. GFP adds approximately 27 kDa to the total size of the respective protein. FUS-GFP and EWS-GFP are expressed at slightly augmented levels compared with their endogenous counterparts while TAF15-GFP is expressed at much higher levels than wild type TAF15.<p><b>Copyright information:</b></p><p>Taken from "The multifunctional FUS, EWS and TAF15 proto-oncoproteins show cell type-specific expression patterns and involvement in cell spreading and stress response"</p><p>http://www.biomedcentral.com/1471-2121/9/37</p><p>BMC Cell Biology 2008;9():37-37.</p><p>Published online 11 Jul 2008</p><p>PMCID:PMC2478660.</p><p></p

The multifunctional FUS, EWS and TAF15 proto-oncoproteins show cell type-specific expression patterns and involvement in cell spreading and stress response-4

Ity in FET expression levels. FET expression is elevated in proliferating endometrium (upper panel) compared to differentiated secretory endometrium (lower panel).<p><b>Copyright information:</b></p><p>Taken from "The multifunctional FUS, EWS and TAF15 proto-oncoproteins show cell type-specific expression patterns and involvement in cell spreading and stress response"</p><p>http://www.biomedcentral.com/1471-2121/9/37</p><p>BMC Cell Biology 2008;9():37-37.</p><p>Published online 11 Jul 2008</p><p>PMCID:PMC2478660.</p><p></p

The multifunctional FUS, EWS and TAF15 proto-oncoproteins show cell type-specific expression patterns and involvement in cell spreading and stress response-7

SC – serous cells.<p><b>Copyright information:</b></p><p>Taken from "The multifunctional FUS, EWS and TAF15 proto-oncoproteins show cell type-specific expression patterns and involvement in cell spreading and stress response"</p><p>http://www.biomedcentral.com/1471-2121/9/37</p><p>BMC Cell Biology 2008;9():37-37.</p><p>Published online 11 Jul 2008</p><p>PMCID:PMC2478660.</p><p></p

The multifunctional FUS, EWS and TAF15 proto-oncoproteins show cell type-specific expression patterns and involvement in cell spreading and stress response-6

Selected mRNAs in samples from inner or peripheral parts of colonies analyzed by quantitative real-time PCR with as endogenous control. – pluripotency marker,– ectodermal marker, – mesodermal marker,– proliferation marker. The day 12 measurement consists of one sample taken from inseparable, mixed cell populations. Undifferentiated hESCs show positive FET protein staining. Cells were stained with primary antibodies for FET proteins and visualized with Cy3-conjugated secondary antibodies (red). The merge images additionally show DAPI staining of nuclei (blue).<p><b>Copyright information:</b></p><p>Taken from "The multifunctional FUS, EWS and TAF15 proto-oncoproteins show cell type-specific expression patterns and involvement in cell spreading and stress response"</p><p>http://www.biomedcentral.com/1471-2121/9/37</p><p>BMC Cell Biology 2008;9():37-37.</p><p>Published online 11 Jul 2008</p><p>PMCID:PMC2478660.</p><p></p

The multifunctional FUS, EWS and TAF15 proto-oncoproteins show cell type-specific expression patterns and involvement in cell spreading and stress response-0

SC – serous cells.<p><b>Copyright information:</b></p><p>Taken from "The multifunctional FUS, EWS and TAF15 proto-oncoproteins show cell type-specific expression patterns and involvement in cell spreading and stress response"</p><p>http://www.biomedcentral.com/1471-2121/9/37</p><p>BMC Cell Biology 2008;9():37-37.</p><p>Published online 11 Jul 2008</p><p>PMCID:PMC2478660.</p><p></p

Uncropped western blot.

(ZIP)</p

Clinical characteristics of the patients included in the study.

(DOCX)</p

Expression of ACE2, TMPRSS2 and NRP1 in clear cell, papillary and chromophobe renal cell carcinoma and across 32 distinct cancer forms.

(A) Bioinformatic analysis of the TCGA data sets for renal cell carcinoma showing mRNA expression levels for the ACE2, TMPRSS2 and NRP1 genes in individual RCC cases for chromophobe renal cell carcinoma (CHRCC), clear cell renal cell carcinoma (CCRCC) and papillary renal cell carcinoma (PRCC). (B) Comparison of the mRNA expression levels for ACE2, TMPRSS2 and NRP1 across 32 different types of malignancies deposited at the TCGA atlas. KIRC denotes CCRCC (red square), KIRP denotes PRCC (blue square) and KICH denotes CHRCC (green square). (C) Immunoblotting results for ACE2, TMPRSS2 and NRP1 proteins in lysates from normal tissue, CCRCC, PRCC and CHRCC. Results from densitometry of the protein bands is shown below the blots.</p