Remote Sensing Applications in Monitoring of Protected Areas

Protected areas (PAs) have been established worldwide for achieving long-term goals in the conservation of nature with the associated ecosystem services and cultural values. Globally, 15% of the world’s terrestrial lands and inland waters, excluding Antarctica, are designated as PAs. About 4.12% of the global ocean and 10.2% of coastal and marine areas under national jurisdiction are set as marine protected areas (MPAs). Protected lands and waters serve as the fundamental building blocks of virtually all national and international conservation strategies, supported by governments and international institutions. Some of the PAs are the only places that contain undisturbed landscape, seascape and ecosystems on the planet Earth. With intensified impacts from climate and environmental change, PAs have become more important to serve as indicators of ecosystem status and functions. Earth’s remaining wilderness areas are becoming increasingly important buffers against changing conditions. The development of remote sensing platforms and sensors and the improvement in science and technology provide crucial support for the monitoring and management of PAs across the world. In this editorial paper, we reviewed research developments using state-of-the-art remote sensing technologies, discussed the challenges of remote sensing applications in the inventory, monitoring, management and governance of PAs and summarized the highlights of the articles published in this Special Issue

Dual-modal tracking of transplanted mesenchymal stem cells after myocardial infarction

Yefei Li*, Yuyu Yao*, Zulong Sheng, Yanxiaoxiao Yang, Genshan Ma,Department of Cardiology, Zhongda Hospital, Medical School of Southeast University, Nanjing, Jiangsu, China*These two authors contributed equally to this work.Purpose: Results for implantation efficiency and effective improvement of cardiac function in the field of mesenchymal stem cells (MSCs) are controversial. To attempt to clarify this debate, we utilized magnetic resonance imaging (MRI) and near-infrared optical imaging (OI) to explore the effects of different delivery modes of mesenchymal stem cells on cell retention time and cardiac function after myocardial infarction (MI).Methods: Rat MSCs were labeled with superparamagnetic iron oxide nanoparticles and 1, 1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt (DiD) for noninvasive cell tracking in a rat MI model. Rats underwent coronary artery ligation and were randomized into three experimental groups: intravenous (IV), intramyocardial (IM), and a control group. The first two groups referred to the route of delivery of the transplanted dual-labeled MSCs; whereas the control group was given an IV injection of serum-free medium one day post-MI. Cellular engraftment was determined 1 day and 7 days post cell delivery by measuring the iron and optical signals in explanted organs. Prussian blue staining and fluorescent microscopy were performed on histological sections for iron and DiD, respectively. Cardiac function was measured by echocardiography on day 7.Results: The cardiac function of the IM group increased significantly compared to the IV and control groups at day 7. In the IM group, labeled cells were visualized in the infracted heart by serial MRI, and the intensity by OI was significantly higher on day 1. In the IV group, the heart signals were significantly attenuated by dual-modal tracking at two time points, but the lung signals in OI were significantly stronger than the IM group at both time points.Conclusion: IM injection of MSCs increased cell engraftment within infarcted hearts and improved cardiac function after MI. However, IV infusion has a low efficacy due to the cell trapping in the lung. Therefore, direct injection may provide an advantage over IV, with regard to retention of stem cells and protection of cardiac function.Keywords: stem cell tracking, superparamagnetic iron oxide, DiD, cardiac function, myocardial infarctio

Analysis of Travel Hot Spots of Taxi Passengers Based on Community Detection

It is an important content of smart city research to study the activity track of urban residents, dig out the hot spot areas and spatial interaction patterns of different residents’ activities, and clearly understand the travel rules of urban residents' activities. This study used community detection to analyze taxi passengers’ travel hot spots based on taxi pick-up and drop-off data, combined with multisource information such as land use, in the main urban area of Nanjing. The study revealed that, for the purpose of travel, the modularity and anisotropy rate of the community where the passengers were picked up and dropped off were positively correlated during the morning and evening peak hours and negatively correlated at other times. Depending on the community structure, pick-up and drop-off points reached significant aggregation within the community, and interactions among the communities were also revealed. Based on the type of land use, as passengers' travel activity increased, travel hot spots formed clusters in urban spaces. After comparative verification, the results of this study were found to be accurate and reliable and can provide a reference for urban planning and traffic management

Impact of Air Pollution on Short-Term Movements: Evidence from Air Travels in China

10.1093/jeg/lbaa005Journal of Economic Geography420939 - 96

Hypoxic preconditioning improves survival of cardiac progenitor cells: role of stromal cell derived factor-1α-CXCR4 axis.

BACKGROUND: Cardiac progenitor cells (CPCs) have been shown to be suitable in stem cell therapy for resurrecting damaged myocardium, but poor retention of transplanted cells in the ischemic myocardium causes ineffective cell therapy. Hypoxic preconditioning of cells can increase the expression of CXCR4 and pro-survival genes to promote better cell survival; however, it is unknown whether hypoxia preconditioning will influence the survival and retention of CPCs via the SDF-1α/CXCR4 axis. METHODS AND RESULTS: CPCs were isolated from adult mouse hearts and purified by magnetic activated cell sorting using c-kit magnetic beads. These cells were cultured at various times in either normoxic or hypoxic conditions, and cell survival was analyzed using flow cytometry and the expression of hypoxia-inducible factor-1α (HIF-1α), CXCR4, phosphorylated Akt and Bcl-2 were measured by Western blot. Results showed that the expression of pro-survival genes significantly increased after hypoxia treatment, especially in cells cultured in hypoxic conditions for six hours. Upon completion of hypoxia preconditioning from c-kit+ CPCs for six hours, the anti-apoptosis, migration and cardiac repair potential were evaluated. Results showed a significant enhancement in anti-apoptosis and migration in vitro, and better survival and cardiac function after being transplanted into acute myocardial infarction (MI) mice in vivo. The beneficial effects induced by hypoxia preconditioning of c-kit+ CPCs could largely be blocked by the addition of CXCR4 selective antagonist AMD3100. CONCLUSIONS: Hypoxic preconditioning may improve the survival and retention of c-kit+ CPCs in the ischemic heart tissue through activating the SDF-1α/CXCR4 axis and the downstream anti-apoptosis pathway. Strategies targeting this aspect may enhance the effectiveness of cell-based cardiac regenerative therapy

Bradykinin Preconditioning Improves Therapeutic Potential of Human Endothelial Progenitor Cells in Infarcted Myocardium

<div><p>Objectives</p><p>Stem cell preconditioning (PC) is a powerful approach in reducing cell death after transplantation. We hypothesized that PC human endothelial progenitor cells (hEPCs) with bradykinin (BK) enhance cell survival, inhibit apoptosis and repair the infarcted myocardium.</p> <p>Methods</p><p>The hEPCs were preconditioned with or without BK. The hEPCs apoptosis induced by hypoxia along with serum deprivation was determined by annexin V-fluorescein isothiocyanate/ propidium iodide staining. Cleaved caspase-3, Akt and eNOS expressions were determined by Western blots. Caspase-3 activity and vascular endothelial growth factor (VEGF) levels were assessed in hEPCs. For <i>in</i><i>vivo</i> studies, the survival and cardiomyocytes apoptosis of transplanted hEPCs were assessed using 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodi- carbocyanine,4-chlorobenzenesul-fonate salt labeled hEPCs and TUNEL staining. Infarct size and cardiac function were measured at 10 days after transplantation, and the survival of transplanted hEPCs were visualized using near-infrared optical imaging.</p> <p>Results</p><p><i>In</i><i>vitro</i> data showed a marked suppression in cell apoptosis following BK PC. The PC reduced caspase-3 activation, increased the Akt, eNOS phosphorylation and VEGF levels. <i>In</i><i>vivo</i> data in preconditioned group showed a robust cell anti-apoptosis, reduction in infarct size, and significant improvement in cardiac function. The effects of BK PC were abrogated by the B2 receptor antagonist HOE140, the Akt and eNOS antagonists LY294002 and L-NAME, respectively.</p> <p>Conclusions</p><p>The activation of B2 receptor-dependent PI3K/Akt/eNOS pathway by BK PC promotes VEGF secretion, hEPC survival and inhibits apoptosis, thereby improving cardiac function <i>in</i><i>vivo</i>. The BK PC hEPC transplantation for stem cell-based therapies is a novel approach that has potential for clinical used. </p> </div