The Role of Alfalfa and Forage Resources in Crop-Livestock Systems in a Rain-Fed Region of North-Western China

Western China has been facing the challenges of both environmental sustainability and economic development. Current government policies and interventions support the development of integrated crop-livestock production systems for enhancing food security and environment sustainability. Compared with traditional grain-based faming systems, integrated systems have better resource utilisation, however annual forage supply deficits estimated at 1.37 t DM forage/farm are experienced in the region, especially in late winter and early spring. Accordingly, optimising the use of available forage resources is a priority for regional researchers and extension officers, with research seeking pathways for better fodder conservation and greater and more effective use of fodder sources to close the spring feed gap. This paper presents relevant research activities relating to alfalfa (Medicago sativa L.) and winter wheat (Triticum aestivum L.) management in integrated crop-livestock production systems. As the planting of alfalfa is strongly encouraged in northern China, alfalfa species selection and optimised harvesting management are essential to ensure the full benefits of alfalfa are available for livestock intensification. The capacity for a prevalent grain crop, winter wheat to be managed as a dual-purpose fodder and grain source shows potential as a means to alleviate deficits of animal feed supply. The present information expands our understanding of integrated forage-livestock production in rain-fed areas, exploring options that can improve productivity in small-farm households leading to income generation

Integrated analysis of miRNAome transcriptome and degradome reveals miRNA-target modules governing floral florescence development and senescence across early- and late-flowering genotypes in tree peony

As a candidate national flower of China, tree peony has extremely high ornamental, medicinal and oil value. However, the short florescence and rarity of early-flowering and late-flowering varieties restrict further improvement of the economic value of tree peony. Specific miRNAs and their target genes engaged in tree peony floral florescence, development and senescence remain unknown. This report presents the integrated analysis of the miRNAome, transcriptome and degradome of tree peony petals collected from blooming, initial flowering, full blooming and decay stages in early-flowering variety Paeonia ostii ‘Fengdan’, an early-flowering mutant line of Paeonia ostii ‘Fengdan’ and late-flowering variety Paeonia suffruticosa ‘Lianhe’. Transcriptome analysis revealed a transcript (‘psu.G.00014095’) which was annotated as a xyloglucan endotransglycosylase/hydrolase precursor XTH-25 and found to be differentially expressed across flower developmental stages in Paeonia ostii ‘Fengdan’ and Paeonia suffruticosa ‘Lianhe’. The miRNA-mRNA modules were presented significant enrichment in various pathways such as plant hormone signal transduction, indole alkaloid biosynthesis, arachidonic acid metabolism, folate biosynthesis, fatty acid elongation, and the MAPK signaling pathway. Multiple miRNA-mRNA-TF modules demonstrated the potential functions of MYB-related, bHLH, Trihelix, NAC, GRAS and HD-ZIP TF families in floral florescence, development, and senescence of tree peony. Comparative spatio-temporal expression investigation of eight floral-favored miRNA-target modules suggested that transcript ‘psu.T.00024044’ and microRNA mtr-miR166g-5p are involved in the floral florescence, development and senescence associated agronomic traits of tree peony. The results might accelerate the understanding of the potential regulation mechanism in regards to floral florescence, development and abscission, and supply guidance for tree peony breeding of varieties with later and longer florescence characteristics

Anxiety, depression, psychological stress and coping style in medical postgraduates in southeastern China when restricted to commuting between the campus and hospital during the COVID-19 pandemic

BackgroundAs the COVID-19 epidemic was gradually brought under control, a new autumn semester began in 2020. How was the mental health of postgraduates as they experienced quarantine at home, only commuting between the school and hospital?MethodsThe research was conducted in a cross-sectional online survey in October 2020. The data were collected from 1,645 medical postgraduates (master’s and doctoral students) by using the demographic information questionnaire, the Self-rating Depression Scale (SDS), the Self-rating Anxiety Scale (SAS), the Questionnaire on Psychological Stressors of Postgraduates (QPSP), the Simplified Coping Style Questionnaire (SCSQ) and the Social Support Rate Scale (SSRS). One-way ANOVA and Pearson correlation were used to explore the relationships among anxiety, depression, psychological stressors, social support and coping style. Structural equation modeling (SEM) was conducted to assess the mediation model.ResultsAmong the total of 1,645 medical postgraduates, 21.6% (n = 356) had self-rated depression symptoms, and 9.4% (n = 155) had self-rated anxiety symptoms. The main disturbances they experienced were employment, academic and interpersonal pressure. The master of third grade students had the highest employment pressure, and the master of second grade students had the highest academic and interpersonal pressure. Negative coping played a negative mediating role and social support played a positive mediating role in the relationships between perceived stress and anxiety (β = 0.027, P < 0.01; β = 0.124, P < 0.01) and depression (β = 0.016, P < 0.01; β = 0.193, P < 0.01).ConclusionMedical postgraduates in China restricted to studies on campus and in the hospital experienced psychological distress. Our results suggest that providing employment and learning guidance, while strengthening social support and guiding positive coping may be effective at improving the mental health of the medical graduate students, mediating their perceived stress and negative emotions

Joint Associations of Maternal Gestational Diabetes and Hypertensive Disorders of Pregnancy With Overweight in Offspring

Objectives: Either maternal gestational diabetes mellitus (GDM) or hypertensive disorder of pregnancy (HDP) is associated with an increased risk of obesity in the offspring. However, their joint associations with obesity in offspring remain unclear. We investigated the joint associations of maternal GDM and HDP with childhood overweight in offspring.Methods: We performed a large study in 1967 mother-child pairs. Maternal GDM was diagnosed according to the 1999 World Health Organization (WHO) criteria. HDP was defined as self-reported doctor-diagnosed hypertension or treatment of hypertension (including gestational hypertension, preeclampsia, sever preeclampsia or eclampsia) after 20 weeks of gestation on the questionnaire. Body mass index (BMI) for age Z-score and childhood overweight were evaluated according to WHO growth reference. We used the general linear models to compare children's Z score for BMI and logistic regression models to estimate odds ratios of childhood overweight according to maternal different status of GDM and HDP.Results: Offspring of mothers with both GDM and HDP had a higher BMI for age Z-score (0.63 vs. 0.03, P <0.001) than children born to normotensive and normoglycemic pregnancy. After adjustment for maternal and children's major confounding factors, joint GDM and HDP were associated with increased odds ratios of offspring's overweight compared with normotensive and normoglycemic pregnancy (2.97, 95% confidence intervals [CIs] 1.65–5.34) and GDM alone (2.06, 95% CIs 1.20–3.54), respectively. After additional adjustment for maternal pre-pregnancy BMI and gestational weight gain, joint maternal GDM, and HDP was still associated with an increased risk of offspring's overweight compared with the maternal normotensive, and normoglycemic group but became to have a borderline increased risk compared with the maternal GDM alone group.Conclusions: Maternal GDM alone or joint GDM and HDP were associated with increased ratios of offspring's overweight.Peer reviewe

Flow‐induced shear stress and deformation of a core–shell‐structured microcapsule in a microchannel

A numerical model was developed and validated to investigate the fluid–structure interactions between fully developed pipe flow and core–shell-structured microcapsule in a microchannel. Different flow rates and microcapsule shell thicknesses were considered. A sixth-order rotational symmetric distribution of von Mises stress over the microcapsule shell can be observed on the microcapsule with a thinner shell configuration, especially at higher flow rate conditions. It is also observed that when being carried along in a fully developed pipe flow, the microcapsule with a thinner shell tends to accumulate stress at a higher rate compared to that with a thicker shell. In general, for the same microcapsule configuration, higher flow velocity would induce a higher stress level over the microcapsule shell. The deformation gradient was used to capture the microcapsule's deformation in the present study. The effect of Young's modulus on the microcapsule shell on the microcapsule deformation was investigated as well. Our findings will shed light on the understanding of the stability of core–shell-structured microcapsule when subjected to flow-induced shear stress in a microfluidic system, enabling a more exquisite control over the breakup dynamics of drug-loaded microcapsule for biomedical applications

Influence of Synthetic Limestone Sand on the Frost Resistance of Magnesium Potassium Phosphate Cement Mortar

Synthetic limestone sand has advantages, such as stable quality and adjustable particle size distribution, and has gradually substituted high-quality natural sand as a fine aggregate in concrete production. The project team has prepared Magnesium Potassium Phosphate Cement (MKPC) mortar by replacing part of the river sand with machine-made limestone sand in equal amounts, which proves that its physical and mechanical properties are obviously better than mortar prepared by whole river sand. However, the research on the impact of machine-made limestone sand on the durability of MKPC mortar has not been carried out. As the repairing material of concrete structures, the frost resistance of MKPC mortar must be evaluated. In this study, the effect of synthetic limestone sand on the frost resistance of Magnesium Potassium Phosphate Cement (MKPC) mortar was investigated by characterizing the strength, mass loss rate, and water absorption of specimens subjected to freeze&ndash;thaw cycling. MKPC mortars prepared using solely river sand (M0) or limestone sand (M1) were completely degraded after 225 freezing&ndash;thawing cycles in water, whereas the flexural and compressive strengths of MKPC mortar (M2) prepared using both river and synthetic limestone sands was 29.3 and 22.0% of the initial strengths, respectively. The water freeze&ndash;thaw resistance of M2 specimens were significantly higher than that of M0 and M1 specimens, and the sulfate freeze&ndash;thaw resistance of M1 and M2 were significantly higher than that of M0. The mass loss of MKPC mortar is not more than 0.4% when it is frozen and thawed 225 times in water and 5% Na2SO4 solution, which is far lower than the damage standard of 5%. Based on the favorable composition of the two aggregates, the initial open porosity of M2 was relatively low, owing to the lower water&ndash;cement ratio of the mortar at the same flow rate

Glucose metabolism among obese and non-obese children of mothers with gestational diabetes

ObjectivesAbdominal obesity is more closely associated with diabetes than general obesity in adults, however, it is unknown which kind of obesity is more closely associated with abnormal glucose metabolism in children.Research design and methodsWe recruited 973 children (aged 3.08±1.06) of mothers with prior gestational diabetes mellitus (GDM). Children’s height, weight, waist circumstance, fasting glucose and insulin were measured using standardized methods. Logistic regression models were used to assess the single and joint associations of general and abdominal obesity with the risks of hyperglycemia (the upper quartile of fasting glucose), insulin resistance (the upper quartile of homeostatic model assessment of insulin resistance (HOMA-IR)), and β-cell dysfunction (the lower quartile of HOMA-%β).ResultsCompared with normal weight children, children with general overweight/obesity had higher levels of HOMA-IR and HOMA-%β, higher ORs for hyperglycemia (1.56, 95% CI 1.06 to 2.30) and insulin resistance (3.44, 95% CI 2.32 to 5.09), but a lower OR for β-cell dysfunction (0.65, 95% CI 0.41 to 1.04). Children with abdominal obesity had an increased risk of insulin resistance (2.54, 95% CI 1.71 to 3.76) but not hyperglycemia and β-cell dysfunction compared with children with normal waist circumstance. In the joint analyses, general overweight children with and without abdominal obesity had an increased risk of hyperglycemia and insulin resistance compared with normal weight children.ConclusionsGeneral obesity was more closely associated with abnormal glucose metabolism than abdominal obesity in children of mothers with GDM

Development and Characterization of Microsatellite Markers Based on the Chloroplast Genome of Tree Peony

Tree peony (Paeonia suffruticosa Andr.) is a famous ornamental and medicinal flowering species. However, few high-efficiency chloroplast microsatellite markers have been developed for it to be employed in taxonomic identifications and evaluation of germplasm resources to date. In the present study, a total of 139 cpSSR loci were identified across eleven tree peony plastomes. Dinucleotide repeat SSRs (97.12%) were most abundantly repeated for the AT motif (58.27%), followed by the TA motif (30.94%) and the TC motif (7.91%). Twenty-one primer pairs were developed, and amplification tests were conducted for nine tree peony individuals. Furthermore, 19 cpSSR markers were amplified on 60 tree peony accessions by a capillary electrophoresis test. Of 19 cpSSR markers, 12 showed polymorphism with different alleles ranging from 1.333 to 3.000. The Shannon&rsquo;s information index and polymorphism information content values ranged from 0.038 to 0.887 (mean 0.432) and 0.032 to 0.589 (mean 0.268), respectively. The diversity levels for twelve loci ranged from 0.016 (at loci cpSSR-8 and cpSSR-26) to 0.543 (at locus cpSSR-15), averaging 0.268 for all loci. A total of 14 haplotypes (23.33%) were detected in the three populations. The haplotypic richness ranged from 0.949 to 1.751, with a mean of 1.233 per population. The genetic relationship suggested by the neighbor-joining-based dendrogram divided the genotypes into two clusters. The Jiangnan population was allotted to Cluster II, and the other two populations were distributed into both branches. These newly developed cpSSRs can be utilized for future breeding programs, population genetics investigations, unraveling the genetic relationships between related species, and germplasm management

Phase change materials meet microfluidic encapsulation

Improving the utilization of thermal energy is crucial in the world nowadays due to the 23 high levels of energy consumption. One way to achieve this is to use phase change materials 24 (PCMs) as thermal energy storage media. PCMs can efficiently store and release thermal energy 25 in the form of latent heat, which can be used to regulate temperature or provide heating/cooling in 26 various applications. This can lead to reduced energy consumption and costs, making PCMs a 27 promising solution to promote sustainable energy usage. However, PCMs have limitations like 28 low thermal conductivity, leakage, and corrosion. To overcome these challenges, PCMs are 29 encapsulated into microencapsulated phase change materials (MEPCMs) capsules/fibers. This 30 encapsulation prevents leakage and corrosion issues, ensuring the PCM remains contained and 31 functional, and the microcapsules/fibers act as conduits for heat transfer, enabling efficient 32 exchange between the PCM and its surroundings. Microfluidics-based MEPCMs has attracted 33 intensive attention over the past decade due to the exquisite control over flow conditions and size 34 of microcapsules. This review paper aims to provide an overview of the state-of-art progress in 35 microfluidics-based encapsulation of PCMs. The principle and method of preparing MEPCM 3

Characteristics of PoVIN3, a Key Gene of Vernalization Pathway, Affects Flowering Time

The tree peony (Paeonia section Moutan DC.) is the candidate flower in China, with abundant germplasm resources and high ornamental value. However, the short and concentrated flowering period severely restricted the improvement of the economic value of tree peonies. Based on the full-length transcriptome database of tree peonies, the PoVIN3 (GenBank ID: OP341879), involved in the flowering regulation of tree peonies were identified and cloned for the first time. The PoVIN3 was also characterized by bioinformatics methods, quantitative real-time PCR (qRT-PCR), and the establishment of a transgenic system. The expression levels of PoVIN3 in seven different petals developmental stages were the highest at the initial flowering stage of the variant cultivar of Paeonia ostii &lsquo;Fengdan,&rsquo; the initial decay stage of the normal flowering Paeonia ostii &lsquo;Fengdan,&rsquo; and the half opening stage of the late flowering Paeonia suffruticosa &lsquo;Lianhe.&rsquo; Tissue-specific expression analysis showed that the relative expression levels of PoVIN3 were the highest in sepals of both normal flowering Paeonia ostii &lsquo;Fengdan&rsquo; and the late flowering Paeonia suffruticosa &lsquo;Lianhe,&rsquo; and the highest expression was in stamens of early flowering mutant Paeonia ostii &lsquo;Fengdan.&rsquo; In addition, the flowering time of pCAMBIA2300-PoVIN3 transgenic plants was significantly earlier than that of the wild-type, indicating that PoVIN3 could promote plant flowering. The results provide a theoretical basis for exploring the role of PoVIN3 in the regulation of flowering in tree peonies