第828回千葉医学会例会・第6回磯野外科例会 88-3.

Western blotting was performed to examine the protein levels of Twist in the indicated cells; β-actin was used as control. (JPG 151 kb

Effect of emodin on the viability of VSMCs.

<p>The cells were incubated with the different concentrations of emodin for 24 h. Then, the cell viability was assayed by the MTT method. DMSO (0.1%) was used as solvent control. Results from three independent experiments were expressed as mean ± S.E.M. *<i>P</i> < 0.05 <i>vs</i>. DMSO.</p

Effects of emodin on ERK1/2 and p38 phosphorylation and PPARγ expression in the thoracic aorta of hyperhomocysteinemic rats.

<p>Rats were fed with the high methionine diet for 4 weeks, and simultaneously treated with two doses of emodin. In the end of the experiment, the thoracic aorta was removed for detecting ERK1/2 and p38 phosphorylation as well as PPARγ protein expression with Western blot. Data were expressed as mean ± S.E.M. (n = 8). *P < 0.05 vs. control, #P < 0.05 vs. model group.</p

Effects of emodin on Hcy-stimulated CRP expression and ROS generation in VSMCs.

<p>The cells were preincubated with the different concentrations of emodin for 24 h before stimulation with 100 μM homocysteine (Hcy) for 12 h. mRNA (A) and protein (B) expression of CRP was identified by RT-PCR and Western blot, respectively. In another experiment, the cells were incubated with the different concentrations of emodin for 24 h and then, exposed to 100 μM Hcy for 40 min after preloaded with H₂DCF-DA (10 μM) for 30 min. The fluorescent intensity was measured by a fluorescence microscope. (C) Representative fluorescence images: (a) control, (b) Hcy alone, (c) Hcy + 0.1 μM emodin, (d) Hcy + 1 μM emodin, (e) Hcy + 10 μM emodin, (f) 10 μM emodin, (g) 0.1% DMSO. (D) Relative fluorescence intensity quantified from the fluorescence images. Emodin alone was used as drug control and DMSO (0.1%) was used as solvent control. Results from three independent experiments were expressed as means ± S.E.M. *<i>P</i> < 0.05 <i>vs</i>. control, ^<i>#</i><i>P</i> < 0.05 <i>vs</i>. Hcy alone.</p

Effects of emodin on the serum Hcy and CRP levels and CRP expression in the thoracic aorta in hyperhomocysteinemic rats.

<p>Rats were fed with the high methionine diet for 4 weeks, and simultaneously treated with two doses of emodin. In the end of the experiment, the blood was collected for detecting serum Hcy level (A) with the enzyme cycling method and CRP concentration (B) with ELISA. Then, the thoracic aorta was removed for examining mRNA (C) and protein (D) expression of CRP by RT-PCR and Western blot, respectively. Data were expressed as mean ± S.E.M. (n = 8). *<i>P</i> < 0.05 <i>vs</i>. control, #<i>P</i> < 0.05 <i>vs</i>. model group.</p

Indole-to-Carbazole Strategy for the Synthesis of Substituted Carbazoles under Metal-Free Conditions

An efficient indole-to-carbazole strategy has been developed under metal-free conditions. This carbazole formation was highly promoted by NH₄I with high regioselectivity through formal [2 + 2 + 2] annulation of indoles, ketones, and nitroolefins. It thus conveniently enabled the assembly of a large number of diversified carbazole products with good tolerance of a broad range of functional groups

Additional file 1 of Role of hypermethylated SLC5A8 in follicular thyroid cancer diagnosis and prognosis prediction

Additional file 1: Supplemental Figure 1. Comparison of SLC5A8 gene methylation in patients with different clinical features in baseline. Supplemental Figure 2. Radioactive iodine (RAI) treatment of representative cases with thyroid cancer metastasis. (A) whole body imaging and (B) computed tomography (CT) of patients who underwent RAI with lung metastasis+lymph node metastasis. (C) whole body imaging and (D) CT of patients who underwent RAI with bone metastasis. Supplemental Table 1. The baseline characteristics of included participants

One-Pot Cascade Synthesis of Substituted Carbazoles from Indoles, Ketones, and Alkenes Using Oxygen as the Oxidant

An efficient one-pot two-step indole-to-carbazole strategy has been developed. This transition metal-free methodology uses oxygen as the sole oxidant and starts from cheap and readily available indoles, ketones, and alkenes. The present protocol efficiently enables the assembly of a diverse array of substituted carbazole products with good regioselectivity and broad tolerance of functional groups

DataSheet_1_Carbon assimilation and distribution in cotton photosynthetic organs is a limiting factor affecting boll weight formation under drought.docx

Previous studies have documented cotton boll weight reductions under drought, but the relative importance of the subtending leaf, bracts and capsule wall in driving drought-induced reductions in boll mass has received limited attention. To investigate the role of carbon metabolism in driving organ-specific differences in contribution to boll weight formation, under drought conditions. Controlled experiments were carried out under soil relative water content (SRWC) (75 ± 5)% (well-watered conditions, control), (60 ± 5)% (moderate drought) and (45 ± 5)% (severe drought) in 2018 and 2019 with two cultivars Yuzaomian 9110 and Dexiamian 1. Under severe drought, the decreases of photosynthetic rate (Pn) and carbon isotope composition (δ13C) were observed in the subtending leaf, bract and capsule wall, suggesting that carbon assimilation of three organs was restricted and the limitation was most pronounced in the subtending leaf. Changes in the activities of sucrose phosphate synthase (SPS), sucrose synthase (SuSy), invertases as well as the reduction in expression of sucrose transporter (GhSUT1) led to variabilities in the sucrose content of three organs. Moreover, photosynthate distribution from subtending leaf to seeds plus fibers (the components of boll weight) was significantly restricted and the photosynthetic contribution rate of subtending leaf to boll weight was decreased, while contributions of bracts and capsule wall were increased by drought. This, in conjunction with the observed decreases in boll weight, indicated that the subtending leaf was the most sensitive photosynthetic organ to drought and was a dominant driver of boll weight loss under drought. Therefore, the subtending leaf governs boll weight loss under drought due to limitations in carbon assimilation, perturbations in sucrose metabolism and inhibition of sucrose transport.</p