2 research outputs found
Structural Characterization of Myoglobin Molecules Adsorbed within Mesoporous Silicas
In the present study,
we examined the secondary and tertiary structure of myoglobin (Mb)
within folded sheets mesoporous material (FSM)- and Santa Barbara
amorphous (SBA)-type mesoporous silicas. The Barrett–Joyner–Halenda
pore diameters of SBA-type mesoporous silicas were 39, 70, and 75
Ă…, and that of FSM-type mesoporous silica was 40 Ă…. The
secondary and tertiary structures of myoglobin were observed by Fourier
transform infrared (FTIR) and small-angle neutron scattering (SANS),
respectively. The FTIR and SANS results indicated preservation of
the secondary and tertiary structures of myoglobin inside the pores
of SBA-type mesoporous silicas. Adsorption of myoglobin within FSM-type
mesoporous silica, however, resulted in perturbation of the tertiary
structure, accompanied by partial unfolding of the secondary structure.
Lower structural stability of myoglobin within the FSM-type mesoporous
silica was also confirmed. These findings suggest that the Mb structure
is more influenced by the inner pore surface characteristics than
by geometrical pore size