Waist Circumference is a Better Predictor of Recurrent Pregnancy Loss When Compared to Body Mass Index among Nigerian Women

Recurrent Pregnancy loss is a common obstetrics condition with severe physical and psychological consequences to the patients and attending physicians as well. It affects 1-5% of women and up to 50% has no identifiable etiology. Obesity is one of the independent risk factor of this condition; however, the evidence of this association is conflicting. This study aims to further determine that obese women with higher visceral fat are at increased risk of Recurrent Pregnancy Loss. The study was a cross-sectional case-control at a public referral hospital in Kano state Nigeria where 116 women with recurrent pregnancy loss were compared to a comparison control group consisting of 116 normal women. In addition to their socio-demographics, weight, height, BMI, waist circumference, and Hip circumference were collected. It was discovered that Obese BMI range and High-risk value for WC were significantly higher odds of recurrent pregnancy loss (OR, 1.96; 95% CI, 1.37-2.46; OR, 4.61 95% CI, 3.458- 5.696 respectively) Logistic regression analysis showed that the most important factor predicting the occurrence of Recurrent pregnancy loss was waist circumference. The receiver operator characteristic (ROC) curve showed that WC has a considerably higher area under the curve (AUC) and BMI had the least AUC value. This study concludes that waist circumference may be superior to BMI as a risk factor of Recurrent Pregnancy Loss. Keywords: Recurrent Pregancy Loss, BMI, Waist Circumferenc

Growth Performance and Carcass Merit of Japanese Quails (Coturnix japonica) Fed with Sorghum as an Energy Source Substitute for Maize in North Western Nigeria

A feeding trial was conducted to determine the growth performance and carcass characteristics of Japanese quails fed diets containing sorghum. A total of ninety of one-week old Japanese quail chicks were used for the experiment. Five diets were formulated in which sorghum was included at graded levels 0, 15, 30, 45 and 60% dietary levels designated as treatment 1, 2, 3, 4 and 5 respectively. The experimental period was five weeks when the birds reach six weeks of age.  The results of the growth performance showed significant differences (P<0.05) in the final body weight (161.70 – 180.10 g) and daily feed intake (21.34–25.12 g). Significant (P<0.05) differences was also observed in all carcass parameters measured except for dressing percentage. Liver weight and large intestine with T5 (65% total replacement) recorded the highest means value of 6.00 g and 6.66 g. Non-significant high values of feed conversion ratio were also obtained among the treatments. Treatment 1 (0%) and Treatment 5 (60%) showed better results in all parameters measured compared to other treatments. In conclusions, sorghum grain can play a significant role in formulating quail feed and can completely (60%) be used to replace maize without affecting the performance and carcass yield of the quails

Colonisation of hospital surfaces from low- and middle-income countries by extended spectrum β-lactamase- and carbapenemase-producing bacteria

Hospital surfaces can harbour bacterial pathogens, which may disseminate and cause nosocomial infections, contributing towards mortality in low- and middle-income countries (LMICs). During the BARNARDS study, hospital surfaces from neonatal wards were sampled to assess the degree of environmental surface and patient care equipment colonisation by Gram-negative bacteria (GNB) carrying antibiotic resistance genes (ARGs). Here, we perform PCR screening for extended-spectrum β-lactamases (blaCTX-M-15) and carbapenemases (blaNDM, blaOXA-48-like and blaKPC), MALDI-TOF MS identification of GNB carrying ARGs, and further analysis by whole genome sequencing of bacterial isolates. We determine presence of consistently dominant clones and their relatedness to strains causing neonatal sepsis. Higher prevalence of carbapenemases is observed in Pakistan, Bangladesh, and Ethiopia, compared to other countries, and are mostly found in surfaces near the sink drain. Klebsiella pneumoniae, Enterobacter hormaechei, Acinetobacter baumannii, Serratia marcescens and Leclercia adecarboxylata are dominant; ST15 K. pneumoniae is identified from the same ward on multiple occasions suggesting clonal persistence within the same environment, and is found to be identical to isolates causing neonatal sepsis in Pakistan over similar time periods. Our data suggests persistence of dominant clones across multiple time points, highlighting the need for assessment of Infection Prevention and Control guidelines

Neonatal sepsis and mortality in low-income and middle-income countries from a facility-based birth cohort: an international multisite prospective observational study

Background Neonatal sepsis is a primary cause of neonatal mortality and is an urgent global health concern, especially within low-income and middle-income countries (LMICs), where 99% of global neonatal mortality occurs. The aims of this study were to determine the incidence and associations with neonatal sepsis and all-cause mortality in facility-born neonates in LMICs. Methods The Burden of Antibiotic Resistance in Neonates from Developing Societies (BARNARDS) study recruited mothers and their neonates into a prospective observational cohort study across 12 clinical sites from Bangladesh, Ethiopia, India, Pakistan, Nigeria, Rwanda, and South Africa. Data for sepsis-associated factors in the four domains of health care, maternal, birth and neonatal, and living environment were collected for all mothers and neonates enrolled. Primary outcomes were clinically suspected sepsis, laboratory-confirmed sepsis, and all-cause mortality in neonates during the first 60 days of life. Incidence proportion of livebirths for clinically suspected sepsis and laboratory-confirmed sepsis and incidence rate per 1000 neonate-days for all-cause mortality were calculated. Modified Poisson regression was used to investigate factors associated with neonatal sepsis and parametric survival models for factors associated with all-cause mortality. Findings Between Nov 12, 2015 and Feb 1, 2018, 29 483 mothers and 30 557 neonates were enrolled. The incidence of clinically suspected sepsis was 166·0 (95% CI 97·69–234·24) per 1000 livebirths, laboratory-confirmed sepsis was 46·9 (19·04–74·79) per 1000 livebirths, and all-cause mortality was 0·83 (0·37–2·00) per 1000 neonate-days. Maternal hypertension, previous maternal hospitalisation within 12 months, average or higher monthly household income, ward size (>11 beds), ward type (neonatal), living in a rural environment, preterm birth, perinatal asphyxia, and multiple births were associated with an increased risk of clinically suspected sepsis, laboratory-confirmed sepsis, and all-cause mortality. The majority (881 [72·5%] of 1215) of laboratory-confirmed sepsis cases occurred within the first 3 days of life. Interpretation Findings from this study highlight the substantial proportion of neonates who develop neonatal sepsis, and the high mortality rates among neonates with sepsis in LMICs. More efficient and effective identification of neonatal sepsis is needed to target interventions to reduce its incidence and subsequent mortality in LMICs. Funding Bill & Melinda Gates Foundation

Effects of antibiotic resistance, drug target attainment, bacterial pathogenicity and virulence, and antibiotic access and affordability on outcomes in neonatal sepsis: an international microbiology and drug evaluation prospective substudy (BARNARDS)

Background Sepsis is a major contributor to neonatal mortality, particularly in low-income and middle-income countries (LMICs). WHO advocates ampicillin–gentamicin as first-line therapy for the management of neonatal sepsis. In the BARNARDS observational cohort study of neonatal sepsis and antimicrobial resistance in LMICs, common sepsis pathogens were characterised via whole genome sequencing (WGS) and antimicrobial resistance profiles. In this substudy of BARNARDS, we aimed to assess the use and efficacy of empirical antibiotic therapies commonly used in LMICs for neonatal sepsis. Methods In BARNARDS, consenting mother–neonates aged 0–60 days dyads were enrolled on delivery or neonatal presentation with suspected sepsis at 12 BARNARDS clinical sites in Bangladesh, Ethiopia, India, Pakistan, Nigeria, Rwanda, and South Africa. Stillborn babies were excluded from the study. Blood samples were collected from neonates presenting with clinical signs of sepsis, and WGS and minimum inhibitory concentrations for antibiotic treatment were determined for bacterial isolates from culture-confirmed sepsis. Neonatal outcome data were collected following enrolment until 60 days of life. Antibiotic usage and neonatal outcome data were assessed. Survival analyses were adjusted to take into account potential clinical confounding variables related to the birth and pathogen. Additionally, resistance profiles, pharmacokinetic–pharmacodynamic probability of target attainment, and frequency of resistance (ie, resistance defined by in-vitro growth of isolates when challenged by antibiotics) were assessed. Questionnaires on health structures and antibiotic costs evaluated accessibility and affordability. Findings Between Nov 12, 2015, and Feb 1, 2018, 36 285 neonates were enrolled into the main BARNARDS study, of whom 9874 had clinically diagnosed sepsis and 5749 had available antibiotic data. The four most commonly prescribed antibiotic combinations given to 4451 neonates (77·42%) of 5749 were ampicillin–gentamicin, ceftazidime–amikacin, piperacillin–tazobactam–amikacin, and amoxicillin clavulanate–amikacin. This dataset assessed 476 prescriptions for 442 neonates treated with one of these antibiotic combinations with WGS data (all BARNARDS countries were represented in this subset except India). Multiple pathogens were isolated, totalling 457 isolates. Reported mortality was lower for neonates treated with ceftazidime–amikacin than for neonates treated with ampicillin–gentamicin (hazard ratio [adjusted for clinical variables considered potential confounders to outcomes] 0·32, 95% CI 0·14–0·72; p=0·0060). Of 390 Gram-negative isolates, 379 (97·2%) were resistant to ampicillin and 274 (70·3%) were resistant to gentamicin. Susceptibility of Gram-negative isolates to at least one antibiotic in a treatment combination was noted in 111 (28·5%) to ampicillin–gentamicin; 286 (73·3%) to amoxicillin clavulanate–amikacin; 301 (77·2%) to ceftazidime–amikacin; and 312 (80·0%) to piperacillin–tazobactam–amikacin. A probability of target attainment of 80% or more was noted in 26 neonates (33·7% [SD 0·59]) of 78 with ampicillin–gentamicin; 15 (68·0% [3·84]) of 27 with amoxicillin clavulanate–amikacin; 93 (92·7% [0·24]) of 109 with ceftazidime–amikacin; and 70 (85·3% [0·47]) of 76 with piperacillin–tazobactam–amikacin. However, antibiotic and country effects could not be distinguished. Frequency of resistance was recorded most frequently with fosfomycin (in 78 isolates [68·4%] of 114), followed by colistin (55 isolates [57·3%] of 96), and gentamicin (62 isolates [53·0%] of 117). Sites in six of the seven countries (excluding South Africa) stated that the cost of antibiotics would influence treatment of neonatal sepsis

Economics of fish farming in Ibadan Metropolis

The study assesses the economics of fish farming in Ibadan Metropolis. The data for the study were collected from 50 fish farmers with the aid of structured questionnaires. The data were analyses using descriptive, gross margin and regression techniques. The study revealed that most farmers had secondary education and above. Most farmers operate at small-scale level with an average of 3 ponds in this category. Fish farmers practiced poly culture fish farming. Clarias spp is the most raised fish species followed by Heteroclaria spp. The gross margin analysis revealed that medium scale farmers derived the highest return of 1.55 for every one naira expended. This is followed by large scale farmers at l.52 for every one naira compared with only 1.34 for every naira spent by small-scale farmers. Fixed inputs, labour, fertilizers, feeds and finger lings significantly affect the value of fish produced. Fish farmers under utilize both labour and fertilizers while feeds and finger lings are over utilized. Key words:  Aquaculture, fish production, Profitability and efficienc

Phytochemical analysis and antimicrobial activity of baobab (Adansonia digitata) leaves and stem bark extracts on Staphylococcus aureus and Escherichia coli

The phytochemical analysis and antibacterial activity of methanolic and ethanolic leaf and stem bark extracts of baobab tree on Escherichia coli and Staphylococcus aureus were carried out using agar well diffusion method. The clinical bacterial isolates of Escherichia coli and Staphylococcus aureus were obtained from Microbiology laboratory, Kaduna State University, Kaduna. The bacteria isolates were re-confirmed and identified based on their morphology, cultural characteristics and biochemical tests. The bacteria isolates were confirmed to be Escherichia coli and Staphylococcus aureus. The phytochemical analysis revealed the presence of Alkaloids, Saponins, Flavonoids, Tannins and Terpenoids. The methanolic leaf extract showed a wide range of activity on test isolates, with varying zones of inhibitions as 12 mm, 10 mm, 7 mm, and 4 mm against Staphylococcus aureus and 13 mm, 9 mm, 7 mm, and 3 mm against Escherichia coli at concentration of 1000 mg/ml, 500 mg/ml, 200 mg/ml and 100 mg/ml respectively. The ethanolic leaf extract also showed a wide range of activity on test isolates with varying zones of inhibitions, such as 11mm, 6mm, 5mm and 3mm against S. aureus and 8mm, 7mm, 5mm, and 4mm against E. coli at the concentration of 1000 mg/ml, 500mg/ml, 200 mg/ml and 100mg/ml for each respectively. The methanolic stem bark extract showed less antibacterial activity against the test isolates with the inhibition of 5mm and 4mm against S. aureus and 4mm and 3mm against E.coli at concentration of 1000 mg/ml and 500 mg/ml respectively with no zones of inhibition at concentration of 200 mg/ml and 100mg/ml. The ethanolic stem bark extract also showed no antibacterial activity with no zones of inhibition against the test isolates at concentration of 1000 mg/ml, 500 mg/ml, 200mg/ml and 100 mg/ml. The methanolic leaf extract inhibited the growth of S. aureus and E.coli at concentration of 100 mg/ml with minimum bactericidal concentration at 100 mg/ml. The ethanolic leaf extract inhibited the growth of S. aureus and E. coli at concentration of 100 mg/ml with minimum bactericidal concentration of 100 mg/ml. This result showed that the ethanolic and methanolic leaf extract of Adansonia digitata contained bioactive substances that may likely be used against some pathogenic bacteria when medically used

Effects of poultry waste as an additive in biogas production using cow dung

Globally, there is need for the proper utilization of animal waste products (such as cow dung, poultry waste and so on), which can be used as an alternative source of energy. Herein, the effects of poultry waste as an additive in the production of biogas using substrate (cow dung and poultry waste) and pH value were investigated. The substrate (cow dung and poultry waste) was prepared via simple dry method. Different set up (1, 2, 3,4 and 5) were designed using local materials (based on the floating drum plant type of digester). The gas produced through an anaerobic digestion method of the different set ups were recorded daily at a fixed time (14 hr) for the periods of 10 days. Set up 2 produced the highest volume of gas (89 cm3), a ~2, ~1.1, ~1.4 and ~1.6 -folds enhancement compared with set up 1, 3, 4 and 5, respectively. It observed that the gas produced decrease with an increase of the poultry waste, indicating that poultry waste played significant role in boosting the production of the gas. The pH of the digester and temperature were kept within pH optimum range of 6.5-8.0 and mesophilic temperature range 20-40 oC, respectively. Thus, the substrate developed (2:1 ratio) was economical, non-hazardous and environmentally friendly

Gender preference between traditional and PowerPoint methods of teaching gross anatomy

Introduction: Teaching and learning process is increasingly metamorphosing from the traditional chalk and talk to the modern dynamism in the information and communication technology. Medical education is no exception to this dynamism more especially in the teaching of gross anatomy, which serves as one of the bases of understanding the human structure. Objective: This study was conducted to determine the gender preference of preclinical medical students on the use of traditional (chalk and talk) and PowerPoint presentation in the teaching of gross anatomy. Subjects and Methods: This was cross-sectional and prospective study, which was conducted among preclinical medical students in the University of Maiduguri, Nigeria. Using simple random techniques, a questionnaire was circulated among 280 medical students, where 247 students filled the questionnaire appropriately. The data obtained was analyzed using SPSS version 20 (IBM Corporation, Armonk, NY, USA) to find the method preferred by the students among other things. Results: Majority of the preclinical medical students in the University of Maiduguri preferred PowerPoint method in the teaching of gross anatomy over the conventional methods. The Cronbach alpha value of 0.76 was obtained which is an acceptable level of internal consistency. A statistically significant association was found between gender and preferred method of lecture delivery on the clarity of lecture content where females prefer the conventional method of lecture delivery whereas males prefer the PowerPoint method, On the reproducibility of text and diagram, females prefer PowerPoint method of teaching gross anatomy while males prefer the conventional method of teaching gross anatomy. Conclusion: There are gender preferences with regard to clarity of lecture contents and reproducibility of text and diagram. It was also revealed from this study that majority of the preclinical medical students in the University of Maiduguri prefer PowerPoint presentation over the traditional chalk and talk method in most of the questions ask

Estimation of Turbidity in Water Treatment Plant using Hammerstein-Wiener and Neural Network Technique

Turbidity is a measure of water quality. Excessive turbidity poses a threat to health and causes pollution. Most of the available mathematical models of water treatment plants do not capture turbidity. A reliable model is essential for effective removal of turbidity in the water treatment plant. This paper presents a comparison of Hammerstein Wiener and neural network technique for estimating of turbidity in water treatment plant. The models were validated using an experimental data from Tamburawa water treatment plant in Kano, Nigeria. Simulation results demonstrated that the neural network model outperformed the Hammerstein-Wiener model in estimating the turbidity. The neural network model may serve as a valuable tool for predicting the turbidity in the plant