1 research outputs found
Adsorption Behavior of Lysozyme at Titanium Oxide–Water Interfaces
We
present an in situ X-ray reflectivity study of the adsorption
behavior of the protein lysozyme on titanium oxide layers under variation
of different thermodynamic parameters, such as temperature, hydrostatic
pressure, and pH value. Moreover, by varying the layer thickness of
the titanium oxide layer on a silicon wafer, changes in the adsorption
behavior of lysozyme were studied. In total, we determined less adsorption
on titanium oxide compared with silicon dioxide, while increasing
the titanium oxide layer thickness causes stronger adsorption. Furthermore,
the variation of temperature from 20 to 80 °C yields an increase
in the amount of adsorbed lysozyme at the interface. Additional measurements
with variation of the pH value of the system in a region between pH
2 and 12 show that the surface charge of both protein and titanium
oxide has a crucial role in the adsorption process. Further pressure-dependent
experiments between 50 and 5000 bar show a reduction of the amount
of adsorbed lysozyme with increasing pressure