1 research outputs found
Live-Cell STED Microscopy with Genetically Encoded Biosensor
Of
the various super-resolution techniques, stimulated emission depletion
(STED) microscopy achieves the best temporal resolution at high spatial
resolution, enabling live-cell imaging beyond the diffraction limit.
However, STED and most other super-resolution imaging methods utilize
a particular type of information extractable from the raw data, namely
the positions of fluorophores. To expand on the use of super-resolution
techniques, we report here the live-cell STED microscopy of a dynamic
biosensor. Using the fluorescent H<sub>2</sub>O<sub>2</sub> sensor
HyPer2 for subdiffraction imaging, we were able not only to image
filaments with superior resolution by localizing emission but also
to trace H<sub>2</sub>O<sub>2</sub> produced within living cell by
monitoring brightness of the probe. STED microscopy of HyPer2 demonstrates
potential utility of FP-based biosensors for super-resolution experiments
in situ and in vivo