Developing a Lawyer's Practical Thinking at Higher Education Institution Stage of the Professionalization

The paper considers the particularities of development of a lawyer's practical thinking at the higher education institution stage of professionalization. In order to achieve the set goal, the block of psychological techniques was used, in particular, several scales of R. Cattell's 16-factor personality questionnaire, a questionnaire for diagnosing the practical orientation of thinking, a questionnaire of creative personal capacities etc. The research was conducted with a sample of 282 students of the faculty of law of P.G. Demidov YarSU, with 143 students of the faculty of economics of P.G. Demidov YarSU as the control group (425 people in total). The study performed has shown that the development of qualities of thinking in lawyers at the higher education institution stage of professionalization is distinguished by essential heterochrony. In particular, a significant reduction of most professionally important indices has been found in year 3 of studying at the faculty of law. These indices are restored in years 4 and 5 either in full (intellect) or in part (practical orientation of thinking, development of creative personal capacities). This allows speaking about a crisis in the development of professional thinking of law students in year 3. The consequences of this crisis turn to not be overcome completely by year 5. In the paper, it is supposed that finally the consequences of the crisis found are eliminated as late as during the graduate's direct performing of the professional activity. DOI: 10.5901/mjss.2015.v6n6s5p31

High-Throughput Sequencing of Circulating MicroRNAs in Plasma and Serum during Pregnancy Progression

Although circulating microRNAs (miRNAs) in maternal blood may play an important role in regulation of pregnancy progression and serve as non-invasive biomarkers for different gestation complications, little is known about their profile in blood during normally developing pregnancy. In this study we evaluated the miRNA profiles in paired plasma and serum samples from pregnant women without health or gestational abnormalities at three time points using high-throughput sequencing technology. Sequencing revealed that the percentage of miRNA reads in plasma and serum decreased by a third compared to first and second trimesters. We found two miRNAs in plasma (hsa-miR-7853-5p and hsa-miR-200c-3p) and 10 miRNAs in serum (hsa-miR-203a-5p, hsa-miR-495-3p, hsa-miR-4435, hsa-miR-340-5p, hsa-miR-4417, hsa-miR-1266-5p, hsa-miR-4494, hsa-miR-134-3p, hsa-miR-5008-5p, and hsa-miR-6756-5p), that exhibit level changes during pregnancy (p-value adjusted < 0.05). In addition, we observed differences for 36 miRNAs between plasma and serum (p-value adjusted < 0.05), which should be taken into consideration when comparing the results between studies performed using different biosample types. The results were verified by analysis of three miRNAs using qRT-PCR (p < 0.05). The present study confirms that the circulating miRNA profile in blood changes during gestation. Our results set the basis for further investigation of molecular mechanisms, involved in regulation of pregnancy, and the search for biomarkers of gestation abnormalities