Stainless Steel Electrode for Sensitive Luminol Electrochemiluminescent Detection of H₂O₂, Glucose, and Glucose Oxidase Activity

Electrogenerated chemiluminescence (ECL) application of stainless steel, a robust and cost-effective material, has been developed for the first time. Type 304 stainless steel electrode shows appealing ECL performance in the luminol–H₂O₂ system. It enables the detection of H₂O₂ with a linear range from 1 to 1000 nM and a limit of detection of 0.456 nM [signal-to-noise ratio (S/N) = 3]. The ECL method based on type 304 stainless steel electrode is more sensitive, more cost-effective, and much simpler than other ECL methods reported before. Because the stainless steel electrode has excellent performance for H₂O₂ detection and H₂O₂ participates in many important enzymatic reactions, applications of stainless steel electrode-based ECL for detection of enzyme activities and enzyme substrates were further investigated by use of glucose oxidase (GODx) and glucose as representative enzyme and substrate. The concentrations of glucose and the activity of GODx were directly proportional to ECL intensities over a range of 0.1–1000 μM and 0.001–0.7 units/mL with limits of detection of 0.076 μM and 0.00087 unit/mL (S/N = 3), respectively. This method was successfully used for determining glucose in honey. Because of their remarkable performance and user-friendly features, stainless steel electrodes hold great promise in various electroanalytical applications, such as biosensing, disposable sensors, and wearable sensors

Determination of Concentrated Hydrogen Peroxide Free from Oxygen Interference at Stainless Steel Electrode

H₂O₂ is frequently used at high concentrations in various applications. It is very challenging to detect high concentrations of H₂O₂ and to eliminate oxygen interference for H₂O₂ detection through electrochemical reduction. In the present investigation, the electrochemistry of H₂O₂ at stainless steel electrode has been carried out for the first time. A cathodic peak for H₂O₂ reduction was observed at about −0.40 V, and no cathodic peak for dissolved oxygen reduction was observed on type 304 stainless steel electrode. Amperometric determination of H₂O₂ on type 304 stainless steel electrode displayed a linear range from 0.05 up to 733 mM with a detection limit of 0.02 mM (S/N = 3) and a sensitivity of 16.7 μA mM^–1 cm^–2. The type 304 stainless steel electrode not only shows much higher upper limit than other reported electrodes for the detection of concentrated H₂O₂ but also is free from oxygen interference, which is of great importance for practical applications. This method could detect H₂O₂ in wound wash and lake water with excellent recoveries. Moreover, we successfully applied the stainless steel electrode to determine glucose using glucose oxidase to catalyze the oxidation of glucose to generate hydrogen peroxide. The linear range for glucose is between 0.5 and 25 mM, which covers clinically important blood glucose concentrations well