2 research outputs found

    Germination of the seeds of lettuce using the aquaponic method

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    The present article shows the data about the germination of the seeds of lettuce wetted with various kinds of water, namely, tap water, water from the river, water from fish tanks, and that from a well. The germination rate of the seeds wetted with the tap water was 94 %, with the water from the river β€” 97 %, and with the water from the well β€” 99 %. The highest germination rate was observed in the sample wetted with the water from a fish tank; it reached 100 %. At the end of the experiment the sprouts in the test samples 2.2 times exceeded the reference, by 69.0 and 63.7 %, respectively, which is evidence that germination of the seeds wetted with the water from a fish tank, from the river, and from the well accelerates germination of lettuce seeds. At the end of germination, the average number of leaves in the third and the fourth groups exceeded the reference value by 13.6 %; and in the second group, the number of leaves was greater by 11.4 % than in the reference group. This is evidence that the shoots wetted with the water from the river and a fish tank developed most intensively

    Π’Π›Π˜Π―ΠΠ˜Π• Π€Π•Π ΠœΠ•ΠΠ’ΠΠ’Π˜Π’ΠΠžΠ™ ΠœΠžΠ”Π˜Π€Π˜ΠšΠΠ¦Π˜Π˜ НА Π“Π›Π£Π‘Π˜ΠΠ£ Π“Π˜Π”Π ΠžΠ›Π˜Π—Π ΠœΠžΠ”Π˜Π€Π˜Π¦Π˜Π ΠžΠ’ΠΠΠΠ«Π₯ Π‘Π•Π›ΠšΠžΠ’Π«Π₯ Π˜Π—ΠžΠ›Π―Π’ΠžΠ’

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    This study was aimed at comparing the effect of enzymatic modification of the protein isolates obtained from sunflower cake on their fractional composition. The obtained chromatograms show that electrophoretic fractions of the modified protein isolates are substantially different from the initial protein isolates. The chromatogram of the proteins modified by sunflower sprouts (SS) showed the presence of 11 fractions represented by 17 peaks (with the total area of 39.65 mAUΓ—sec), the chromatogram of a protein isolate modified by whey lactoserum (WLS) β€” of eight fractions and 19 peaks (with the total area of 28.73 mAUΓ—sec), and the chromatogram of a complex enzymatic modification β€” of 13 fractions and 21 peaks (with the total area of 24.20 mAUΓ—sec). Thus, a comprehensive modification caused the differentiation of the first fraction from three peaks with the total relative area of 18.7 % to five peaks with the area of 13.6 % from the total relative area of the peaks of fractions of the corresponding protein product. With that, the comprehensive enzymatic modification and modification of SS resulted in increasing the speed of peaks separation by six minutes, compared to the native protein product, and the modification of WLS reduced the duration by 4.5 minutes. Therefore, reducing the molecular weight and changing the fractional composition of proteins is achieved by the action of proteolytic enzymes of vegetative and microbial origin.Π‘Ρ€Π°Π²Π½Π΅Π½ΠΈΠ΅ влияния Ρ„Π΅Ρ€ΠΌΠ΅Π½Ρ‚Π°Ρ‚ΠΈΠ²Π½ΠΎΠΉ ΠΌΠΎΠ΄ΠΈΡ„ΠΈΠΊΠ°Ρ†ΠΈΠΈ Π±Π΅Π»ΠΊΠΎΠ²Ρ‹Ρ… изолятов, ΠΏΠΎΠ»ΡƒΡ‡Π΅Π½Π½Ρ‹Ρ… ΠΈΠ· подсолнСчного ΠΆΠΌΡ‹Ρ…Π°, Π½Π° ΠΈΡ… Ρ„Ρ€Π°ΠΊΡ†ΠΈΠΎΠ½Π½Ρ‹ΠΉ состав. Из ΠΏΠΎΠ»ΡƒΡ‡Π΅Π½Π½Ρ‹Ρ… Ρ…Ρ€ΠΎΠΌΠ°Ρ‚ΠΎΠ³Ρ€Π°ΠΌΠΌ установлСно, Ρ‡Ρ‚ΠΎ элСктрофорСтичСскиС Ρ„Ρ€Π°ΠΊΡ†ΠΈΠΈ ΠΌΠΎΠ΄ΠΈΡ„ΠΈΡ†ΠΈΡ€ΠΎΠ²Π°Π½Π½Ρ‹Ρ… Π±Π΅Π»ΠΊΠΎΠ²Ρ‹Ρ… изолятов, сущСствСнно ΠΎΡ‚Π»ΠΈΡ‡Π°ΡŽΡ‚ΡΡ ΠΎΡ‚ исходных Π±Π΅Π»ΠΊΠΎΠ²Ρ‹Ρ… изолятов. Π₯Ρ€ΠΎΠΌΠ°Ρ‚ΠΎΠ³Ρ€Π°ΠΌΠΌΠ° Π±Π΅Π»ΠΊΠΎΠ², ΠΌΠΎΠ΄ΠΈΡ„ΠΈΡ†ΠΈΡ€ΠΎΠ²Π°Π½Π½Ρ‹Ρ… РП, ΠΏΠΎΠΊΠ°Π·Π°Π»Π° Π½Π°Π»ΠΈΡ‡ΠΈΠ΅ 11 Ρ„Ρ€Π°ΠΊΡ†ΠΈΠΉ, прСдставлСнных 17 ΠΏΠΈΠΊΠ°ΠΌΠΈ (ΠΎΠ±Ρ‰Π΅ΠΉ ΠΏΡ€Π»ΠΎΡ‰Π°Π΄ΡŒΡŽ 39,65 mAU×сСк), Ρ…Ρ€ΠΎΠΌΠ°Ρ‚ΠΎΠ³Ρ€Π°ΠΌΠΌΠ° Π±Π΅Π»ΠΊΠΎΠ²ΠΎΠ³ΠΎ изолята, ΠΌΠΎΠ΄ΠΈΡ„ΠΈΡ†ΠΈΡ€ΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ ПМБ, - 8 Ρ„Ρ€Π°ΠΊΡ†ΠΈΠΉ ΠΈ 19 ΠΏΠΈΠΊΠΎΠ² (ΠΎΠ±Ρ‰Π΅ΠΉ ΠΏΠ»ΠΎΡ‰Π°Π΄ΡŒΡŽ 28,73 mAΓ—UсСк), Ρ…Ρ€ΠΎΠΌΠ°Ρ‚ΠΎΠ³Ρ€Π°ΠΌΠΌΠ° комплСксной Ρ„Π΅Ρ€ΠΌΠ΅Π½Ρ‚Π°Ρ‚ΠΈΠ²Π½ΠΎΠΉ ΠΌΠΎΠ΄ΠΈΡ„ΠΈΠΊΠ°Ρ†ΠΈΠΈ – 13 Ρ„Ρ€Π°ΠΊΡ†ΠΈΠΉ ΠΈ 21 ΠΏΠΈΠΊ (ΠΎΠ±Ρ‰Π΅ΠΉ ΠΏΠ»ΠΎΡ‰Π°Π΄ΡŒΡŽ 24,20 mAU×сСк). Π’Π°ΠΊΠΈΠΌ ΠΎΠ±Ρ€Π°Π·ΠΎΠΌ, комплСксная модификация Π²Ρ‹Π·Π²Π°Π»Π° Π΄ΠΈΡ„Ρ„Π΅Ρ€Π΅Π½Ρ†ΠΈΠ°Ρ†ΠΈΡŽ ΠΏΠ΅Ρ€Π²ΠΎΠΉ Ρ„Ρ€Π°ΠΊΡ†ΠΈΠΈ с Ρ‚Ρ€Π΅Ρ… ΠΏΠΈΠΊΠΎΠ² с ΠΎΠ±Ρ‰Π΅ΠΉ ΠΎΡ‚Π½ΠΎΡΠΈΡ‚Π΅Π»ΡŒΠ½ΠΎΠΉ ΠΏΠ»ΠΎΡ‰Π°Π΄ΡŒΡŽ 18,7%, Π΄ΠΎ пяти – ΠΏΠ»ΠΎΡ‰Π°Π΄ΡŒΡŽ 13,6%, ΠΎΡ‚ ΠΎΠ±Ρ‰Π΅ΠΉ ΠΎΡ‚Π½ΠΎΡΠΈΡ‚Π΅Π»ΡŒΠ½ΠΎΠΉ ΠΏΠ»ΠΎΡ‰Π°Π΄ΠΈ ΠΏΠΈΠΊΠΎΠ² Ρ„Ρ€Π°ΠΊΡ†ΠΈΠΉ ΡΠΎΠΎΡ‚Π²Π΅Ρ‚ΡΡ‚Π²ΡƒΡŽΡ‰Π΅Π³ΠΎ Π±Π΅Π»ΠΊΠΎΠ²ΠΎΠ³ΠΎ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ‚Π°. ΠŸΡ€ΠΈ этом комплСксная фСрмСнтативная модификация ΠΈ модификация РП ΠΏΡ€ΠΈΠ²Π΅Π»Π° ΠΊ ΡƒΠ²Π΅Π»ΠΈΡ‡Π΅Π½ΠΈΡŽ скорости раздСлСния ΠΏΠΈΠΊΠΎΠ² Π½Π° 6 ΠΌΠΈΠ½ΡƒΡ‚, ΠΏΠΎ ΡΡ€Π°Π²Π½Π΅Π½ΠΈΡŽ с Π½Π°Ρ‚ΠΈΠ²Π½Ρ‹ΠΌ Π±Π΅Π»ΠΊΠΎΠ²Ρ‹ΠΌ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ‚ΠΎΠΌ, Π° модификация ПМБ сократила врСмя Π½Π° 4,5 ΠΌΠΈΠ½ΡƒΡ‚Ρ‹. Π‘Π»Π΅Π΄ΠΎΠ²Π°Ρ‚Π΅Π»ΡŒΠ½ΠΎ, ΡƒΠΌΠ΅Π½ΡŒΡˆΠ΅Π½ΠΈΠ΅ молСкулярной массы ΠΈ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ Ρ„Ρ€Π°ΠΊΡ†ΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ состава Π±Π΅Π»ΠΊΠΎΠ² достигаСтся дСйствиСм протСолитичСских Ρ„Π΅Ρ€ΠΌΠ΅Π½Ρ‚ΠΎΠ² Ρ€Π°ΡΡ‚ΠΈΡ‚Π΅Π»ΡŒΠ½ΠΎΠ³ΠΎ ΠΈ ΠΌΠΈΠΊΡ€ΠΎΠ±Π½ΠΎΠ³ΠΎ происхоТдСния
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