<i>AGG1</i> complements some but not all <i>agg2</i> mutant phenotypes.

<p>(A) Germination dynamics of wild-type, mutant and complementation lines on 0.5x MS supplemented with 6% D-mannitol during 9 days after stratification. Each genotype was analyzed in three replica plates with more than 100 seeds. Insert shows control germination without D-mannitol. (B) Percentage of germinated seeds at day 3 from panel (A) demonstrating highest difference between genotypes. Bars represent average value of three replicates (more than 100 seeds each). Error bars show standard errors. Letters indicate groups with statistically significant differences in seed germination (P<0.05, one-way ANOVA). (C) Total number of lateral roots was scored in two-week-old seedlings grown vertically on 0.5x MS supplemented with 1% sucrose. Bars represent average values ±SE of 15 plants per genotype. Letters indicate groups with statistically significant differences in number of lateral roots (P<0.05, one-way ANOVA). (D, E) <i>AGG1</i> failed to complement the <i>agg2-1</i> mutant on high temperature-induced flowering. (D) Effect of the <i>agg2-1</i> mutation on high temperature-induced flowering. Col-0 and <i>agg2-1</i> plants were initially grown at 22°C for two weeks and then divided into two groups: the first group was kept at 22°C, while the second group was transferred to 29°C. Day of inflorescence appearance was recorded for at least 30 plants of each genotype. Bars represent the average number of days from germination till inflorescence appearance ± SE. Letters indicate groups with statistically significant differences (P<0.05, one-way ANOVA). (E) Average number of days from germination till inflorescence appearance in at least 30 plants of each genotype induced at 29°C. Bars represent the average number of days from germination till inflorescence appearance ± SE. Letters indicate groups with statistically significant differences (P<0.05, one-way ANOVA).</p

Summary of Gγ1 and Gγ2 complementation studies.

<p>Summary of Gγ1 and Gγ2 complementation studies.</p

AGG2 complements some but not all <i>agg1</i> mutant phenotypes.

<p>(A) Sensitivity to <i>F. oxysporum</i>. Roots of two-week-old seedlings were inoculated with <i>F. oxysporum</i> spores and total number and number of chlorotic leaves were counted 9 days after inoculation for each plant. The ratio of chlorotic/total number of leaves was used to evaluate disease progression in infected plants. Bars on the graph represent average values estimated for 20 plants per each genotype. Error bars show standard errors. Letters indicate groups with statistically significant differences in disease progression (P<0.05, one-way ANOVA). (B) Total number of lateral roots was scored in two-week-old seedlings grown vertically on 0.5x MS supplemented with 1% sucrose. Bars represent average values ±SE of 15 plants per genotype. Letters indicate groups with statistically significant differences in number of lateral roots (P<0.05, one-way ANOVA). (C) Adventitious root development in excised hypocotyls was induced by supplementing media with 1µM NAA. Photos of representative hypocotyls from each tested genotype are shown. (D) Germination dynamics of wild-type, mutant and complementation lines grown on 0.5x MS supplemented with 6% D-mannitol during 8 days after stratification. Each genotype was analyzed in three replica plates with more than 100 seeds. Insert shows control germination without D-mannitol. (E) Percentage of germinated seeds at day 8 from panel (D) showing the highest difference between genotypes. Bars represent average value of three replicates (more than 100 seeds each). Error bars show standard errors. Letters indicate groups with statistically significant differences in seed germination (P<0.05, one-way ANOVA). (F) AGG2 partial rescue of the early flowering phenotype observed in <i>agg1-1c</i> mutants. Plants were grown under long day conditions (16 h light/8 h dark) at 23°C. Day of inflorescence appearance was recorded for at least 30 plants of each genotype. Bars represent average number of days from germination till inflorescence appearance ± SE. Letters indicate groups with statistically significant differences (P<0.05, one-way ANOVA).</p