4 research outputs found
Systematic Study in Mammalian Cells Showing No Adverse Response to Tetrahedral DNA Nanostructure
The
advent of DNA technology has demonstrated great potential in a wide
range of applications, especially in the field of biology and biomedicine.
However, current understanding of the toxicological effects and cellular
responses of DNA nanostructures remains to be improved. Here, we chose
tetrahedral DNA nanostructures (TDNs), a type of nanocarriers for
delivering molecular drugs, as a model for systematic live-cell analysis
of the biocompatibility of TDNs to normal bronchial epithelial cells,
carcinoma cells, and macrophage. We found that the interaction behaviors
of TDNs in different cell lines were very different, whereas after
internalization, most of the TDNs in diverse cell lines were positioned
to lysosomes. By a systematic assessment of cell responses after TDN
exposure to various cells, we demonstrate that internalized TDNs have
good innate biocompatibility. Interestingly, we found that TDN-bearing
cells would not affect the cell cycle progression and accompany cell
division and that TDNs were separated equally into two daughter cells.
This study improves our understanding of the interaction of DNA nanostructures
with living systems and their biocompatibility, which will be helpful
for further designing DNA nanostructures for biomedical applications
Systematic Study in Mammalian Cells Showing No Adverse Response to Tetrahedral DNA Nanostructure
The
advent of DNA technology has demonstrated great potential in a wide
range of applications, especially in the field of biology and biomedicine.
However, current understanding of the toxicological effects and cellular
responses of DNA nanostructures remains to be improved. Here, we chose
tetrahedral DNA nanostructures (TDNs), a type of nanocarriers for
delivering molecular drugs, as a model for systematic live-cell analysis
of the biocompatibility of TDNs to normal bronchial epithelial cells,
carcinoma cells, and macrophage. We found that the interaction behaviors
of TDNs in different cell lines were very different, whereas after
internalization, most of the TDNs in diverse cell lines were positioned
to lysosomes. By a systematic assessment of cell responses after TDN
exposure to various cells, we demonstrate that internalized TDNs have
good innate biocompatibility. Interestingly, we found that TDN-bearing
cells would not affect the cell cycle progression and accompany cell
division and that TDNs were separated equally into two daughter cells.
This study improves our understanding of the interaction of DNA nanostructures
with living systems and their biocompatibility, which will be helpful
for further designing DNA nanostructures for biomedical applications
Systematic Study in Mammalian Cells Showing No Adverse Response to Tetrahedral DNA Nanostructure
The
advent of DNA technology has demonstrated great potential in a wide
range of applications, especially in the field of biology and biomedicine.
However, current understanding of the toxicological effects and cellular
responses of DNA nanostructures remains to be improved. Here, we chose
tetrahedral DNA nanostructures (TDNs), a type of nanocarriers for
delivering molecular drugs, as a model for systematic live-cell analysis
of the biocompatibility of TDNs to normal bronchial epithelial cells,
carcinoma cells, and macrophage. We found that the interaction behaviors
of TDNs in different cell lines were very different, whereas after
internalization, most of the TDNs in diverse cell lines were positioned
to lysosomes. By a systematic assessment of cell responses after TDN
exposure to various cells, we demonstrate that internalized TDNs have
good innate biocompatibility. Interestingly, we found that TDN-bearing
cells would not affect the cell cycle progression and accompany cell
division and that TDNs were separated equally into two daughter cells.
This study improves our understanding of the interaction of DNA nanostructures
with living systems and their biocompatibility, which will be helpful
for further designing DNA nanostructures for biomedical applications
Systematic Study in Mammalian Cells Showing No Adverse Response to Tetrahedral DNA Nanostructure
The
advent of DNA technology has demonstrated great potential in a wide
range of applications, especially in the field of biology and biomedicine.
However, current understanding of the toxicological effects and cellular
responses of DNA nanostructures remains to be improved. Here, we chose
tetrahedral DNA nanostructures (TDNs), a type of nanocarriers for
delivering molecular drugs, as a model for systematic live-cell analysis
of the biocompatibility of TDNs to normal bronchial epithelial cells,
carcinoma cells, and macrophage. We found that the interaction behaviors
of TDNs in different cell lines were very different, whereas after
internalization, most of the TDNs in diverse cell lines were positioned
to lysosomes. By a systematic assessment of cell responses after TDN
exposure to various cells, we demonstrate that internalized TDNs have
good innate biocompatibility. Interestingly, we found that TDN-bearing
cells would not affect the cell cycle progression and accompany cell
division and that TDNs were separated equally into two daughter cells.
This study improves our understanding of the interaction of DNA nanostructures
with living systems and their biocompatibility, which will be helpful
for further designing DNA nanostructures for biomedical applications