Safety-oriented planning of expressway truck service areas based on driver demand

Funding This study was supported by the National Natural Science Foundation of China (51978522).Peer reviewedPublisher PD

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Numerical Simulation of Fire Suppression in Stilted Wooden Buildings with Fine Water Mist Based on FDS

In this paper, to reflect a real fire combustion situation of stilted buildings with a typical wooden structure, we used FDS numerical simulation software to study the suppression effect of a fine-water-mist fire-extinguishing system under different working conditions. The influences of different mist droplet diameters, spray flows, and nozzle densities on the temperature change in the combustion area were analyzed and compared. The particle sizes of fog droplets exhibited a significant impact, indicating that the smaller the particle size, the faster the vaporization rate and the better the cooling effect. The cooling effect was better when the particle size was 150 μm or less when compared to the particle sizes of 200 and 300 μm. As the spray flow rate and nozzle density were increased, the fire field temperature decreased, and the cooling effect was enhanced, optimal at a water-mist flow rate of 8 L/min. Therefore, given the possible working conditions, the spray flow rate and the nozzle density should be high, while a suitable droplet size should be selected to achieve the best fire-extinguishing effect

Use of Household Survey Data as a Tool to Assess the Carbon Footprint of Rural Tourist Accommodation and Related Services in China: A Case Study of Mount Qingcheng

The need to improve the accuracy of carbon emission measurements is a major issue which the tourism industry must resolve in order to reduce adverse impacts on climate change and the environment. This study established a detailed consumption list based on household survey data and calculated the carbon emissions of accommodation and services of the rural tourism industry of Mount Qingcheng using the input–output and lifecycle methods. Further, it analysed the key factors affecting carbon emissions. The results indicate that within the surveyed area, carbon emissions from accommodation and services amounted to 30.27 kg CO2/per person per day; these emissions were primarily from indirect sources, which accounted for 74.99% of the total emissions. Emissions from construction and production of durable goods accounted for 13.08% and 21.58% of the total emissions. The omission of these sources of carbon emissions was the primary reason for the carbon emission levels of the tourism industry being underestimated previously. For each additional 10,000 yuan in revenue, accommodation and related services of the rural tourism industry emit an additional 1412.08 kg of CO2. This is higher than the level of carbon emissions of the agriculture industry, but lower than those of the processing and manufacturing industries. Tourist consumption behaviours and types of tourism operations are important factors affecting carbon emissions. Effective emission reduction strategies include guiding tourist consumption behavioural changes, optimizing tourism operation portfolios, and extending the service life of constructions and durable goods

Roles of hypoxic environment and M2 macrophage-derived extracellular vesicles on the progression of non-small cell lung cancer

Abstract Background Hypoxia contributes to the development of invasive and metastatic cancer cells, and is detrimental to cancer treatment. This study aimed to explore the molecular mechanisms by which hypoxic microenvironments affect hypoxic non-small cell lung cancer (NSCLC) development and the effects of M2 macrophage-derived extracellular vesicles (EVs) on NSCLC cells. Methods A549 cells were cultured in an anoxic incubator for 48 h to construct hypoxic A549 cells, and then normal and hypoxic A549 cells were harvested for RNA sequencing. Next, THP-1 cells were used to induce M2 macrophages, and EVs were isolated from THP-1 cells and M2 macrophages. Cell counting kit-8 and transwell assays were used to determine the viability and migration of hypoxic A549 cells, respectively. Results After sequencing, 2426 DElncRNAs and 501 DEmiRNAs were identified in normal A549 cells and hypoxic A549 cells. These DElncRNAs and DEmiRNAs were significantly enriched in “Wnt signaling pathway,” “Hippo signaling pathway,” “Rap1 signaling pathway,” “calcium signaling pathway,” “mTOR signaling pathway,” and “TNF signaling pathway.” Subsequently, ceRNA networks consisting of 4 lncRNA NDRG1 transcripts, 16 miRNAs and 221 target mRNAs were built, and the genes in the ceRNA networks were significantly associated with “Hippo signaling pathway” and “HIF-1 signaling pathway.” EVs were successfully extracted from THP-1 cells and M2 macrophages, and M2 macrophage-derived EVs significantly enhanced the viability and migration of hypoxic A549 cells. Finally, M2 macrophage-derived EVs further upregulated the expression of NDRG1-009, NDRG1-006, VEGFA, and EGLN3, while downregulating miR-34c-5p, miR-346, and miR-205-5p in hypoxic A549 cells. Conclusions M2 macrophage-derived EVs may worsen the progression of NSCLC in a hypoxic microenvironment by regulating the NDRG1-009-miR-34c-5p-VEGFA, NDRG1-006-miR-346-EGLN3, NDRG1-009-miR-205-5p-VEGFA, and Hippo/HIF-1 signaling pathways

Comprehensive Identification of Surface Subsidence Evaluation Grades of Mines in Southwest China

Due to their complex geological structure, it is difficult to systematically analyze the surface subsidence of coal mines in southwest China, and the factors that cause surface subsidence are also different from other coal mines. Focusing on the problem of surface subsidence caused by mining in southwest China’s mines, a grade evaluation system for surface subsidence of southwest mines is constructed based on the analytic hierarchy process, and ten evaluation indicators are established from the perspectives of mining disturbance and geological structure. A matter–element model of surface subsidence based on matter–element extension theory and a cloud model of surface subsidence based on cloud theory are then constructed. A coal mine in Anshun, Guizhou, is taken as an example to calculate the evaluation level of surface subsidence and thus verify the scientificity of extension theory and cloud theory. The results show that the main factors that affect the surface subsidence of southwest mines are the number of coal seam layers, mining height and comprehensive Platt hardness of rock, similar to that of northern plain coal mines. Surface slope and subsidence area are also very important. The comprehensive correlation degree of each grade of the coal mine is −0.29836, 0.192232, −0.1093 and −0.46531, and the coal mine is concluded to be in grade 2. The calculated similarity of the overall index evaluation cloud map of the coal mine and each grade is 0, 0.3453, 0.7872 and 0, respectively. The coal mine is in grade 2, which is a relatively safe state. Consistent with the calculation results of the extension model and in line with the field situation, the extension matter–element model and cloud model built in this paper can verify each other and have a certain scientificity

Comprehensive Identification of Surface Subsidence Evaluation Grades of Mines in Southwest China

Due to their complex geological structure, it is difficult to systematically analyze the surface subsidence of coal mines in southwest China, and the factors that cause surface subsidence are also different from other coal mines. Focusing on the problem of surface subsidence caused by mining in southwest China’s mines, a grade evaluation system for surface subsidence of southwest mines is constructed based on the analytic hierarchy process, and ten evaluation indicators are established from the perspectives of mining disturbance and geological structure. A matter–element model of surface subsidence based on matter–element extension theory and a cloud model of surface subsidence based on cloud theory are then constructed. A coal mine in Anshun, Guizhou, is taken as an example to calculate the evaluation level of surface subsidence and thus verify the scientificity of extension theory and cloud theory. The results show that the main factors that affect the surface subsidence of southwest mines are the number of coal seam layers, mining height and comprehensive Platt hardness of rock, similar to that of northern plain coal mines. Surface slope and subsidence area are also very important. The comprehensive correlation degree of each grade of the coal mine is −0.29836, 0.192232, −0.1093 and −0.46531, and the coal mine is concluded to be in grade 2. The calculated similarity of the overall index evaluation cloud map of the coal mine and each grade is 0, 0.3453, 0.7872 and 0, respectively. The coal mine is in grade 2, which is a relatively safe state. Consistent with the calculation results of the extension model and in line with the field situation, the extension matter–element model and cloud model built in this paper can verify each other and have a certain scientificity

Preparation and representation of recombinant Mn-ferritin flower-like spherical aggregates from marine invertebrates.

Ferritin has important functions in the transition and storage of toxic metal ions, but its regulation and function in many invertebrate species are still largely unknown. In our previous work, the cDNA sequence of Sinonovacula constricta, Apostichopus japonicas and Acaudina leucoprocta were constructed and efficiently expressed in E. Coli BL21 under IPTG induction. In this follow-up study, the recombinant ferritins were exposed to heavy metal manganese. The manganese concentration levels in three recombinant ferritins were greater than horse spleen ferritin (HSF). Compared with HSF, the amount of manganese enrichment in the three recombinant ferritins was 1.75-fold, 3.25-fold and 2.42-fold increases in ScFER, AjFER, and AlFER, respectively. After phosphate stimulation, the concentration of manganese increased and was higher than the ordinary dialysis control groups. The ScFER was four times its baseline value. The AjFER and AlFER were 1.4- and 8-fold higher, respectively. The AlFER sample stimulated by phosphate was 22-fold that of HSF. The morphologies of the resulting Mn-Ferritin from different marine invertebrates were characterized with scanning electron microscopy. Surface morphologies were lamella flower-like and are consistent with changes in surface morphologies of the standard Mn-HSF. Invertebrate recombinant ferritin and HSF both can uptake manganese. We found that the structure of A. leucoproctarecombinant Mn-Ferritin aggregate changed over time. The surface formed lamella flower-like aggregate, but gradually merged to create a relatively uniform plate-like phase of aggregate spherically and fused without clear boundaries

Decreased Salinity Offsets the Stimulation of Elevated pCO2 on Photosynthesis and Synergistically Inhibits the Growth of Juvenile Sporophyte of Saccharina japonica (Laminariaceae, Phaeophyta)

The combined effect of elevated pCO2 (Partial Pressure of Carbon Dioxide) and decreased salinity, which is mainly caused by freshwater input, on the growth and physiological traits of algae has been poorly assessed. In order to investigate their individual and interactive effects on the development of commercially farmed algae, the juvenile sporophytes of Saccharina japonica were cultivated under different levels of salinity (30, 25 and 20 psu) and pCO2 (400 and 1000 µatm). Individually, decreased salinity significantly reduced the growth rate and pigments of S. japonica, indicating that the alga was low-salinity stressed. The maximum quantum yield, Fv/Fm, declined at low salinities independent of pCO2, suggesting that the hyposalinity showed the main effect. Unexpectedly, the higher pCO2 enhanced the maximum relative electron transport rate (rETRmax) but decreased the growth rate, pigments and soluble carbohydrates contents. This implies a decoupling between the photosynthesis and growth of this alga, which may be linked to an energetic reallocation among the different metabolic processes. Interactively and previously untested, the decreased salinity offset the improvement of rETRmax and aggravated the declines of growth rate and pigment content caused by the elevated pCO2. These behaviors could be associated with the additionally decreased pH that was induced by the low salinity. Our data, therefore, unveils that the decreased salinity may increase the risks of future CO2-induced ocean acidification on the production of S. japonica

Acute psychosocial stress weakens the sense of agency in healthy adults

The sense of agency (SoA) refers to the feeling of being in control of one’s actions and the subsequent consequence of these actions. Emotional context seems to alter the strength of sense of agency. The present study explored the influence of acute psychosocial stress on the SoA by means of the Trier Social Stress Test (TSST). Self-assessment manikin (SAM) and objective physiological indicators (e.g. heart rate, electrodermal activity, and salivary cortisol levels) were utilized to evaluate the effect of the TSST. We also employed the temporal binding effect as an implicit assessment of the participant’s SoA. The results indicated that the stress level of the experimental group after TSST was significantly higher than the control group, whilst the temporal binding scores of the experimental group decreased after TSST manipulation. In short, acute psychosocial stress with intense emotional arousal weakened the sense of agency.</p