MOESM1 of Metabolic engineering of Saccharomyces cerevisiae for efficient production of glucaric acid at high titer

Additional file 1: Table S1. Primers used in this study. Figure S1. MIOX4 activity of the episomal expression plasmid in the wild-type strain and opi1 mutant strain with myo-inositol. All experiments were performed in triplicates and the error bar represented mean Âą standard deviation. Figure S2. myo-inositol residue in shake flask (A) and fed-batch (B) cultures when fed 60 mM (10.8 g/L) myo-inositol to the culture. All experiments were performed in triplicates and the error bar represented mean Âą standard deviation