Quantifying Facial Age by Posterior of Age Comparisons

We introduce a novel approach for annotating large quantity of in-the-wild facial images with high-quality posterior age distribution as labels. Each posterior provides a probability distribution of estimated ages for a face. Our approach is motivated by observations that it is easier to distinguish who is the older of two people than to determine the person's actual age. Given a reference database with samples of known ages and a dataset to label, we can transfer reliable annotations from the former to the latter via human-in-the-loop comparisons. We show an effective way to transform such comparisons to posterior via fully-connected and SoftMax layers, so as to permit end-to-end training in a deep network. Thanks to the efficient and effective annotation approach, we collect a new large-scale facial age dataset, dubbed `MegaAge', which consists of 41,941 images. Data can be downloaded from our project page mmlab.ie.cuhk.edu.hk/projects/MegaAge and github.com/zyx2012/Age_estimation_BMVC2017. With the dataset, we train a network that jointly performs ordinal hyperplane classification and posterior distribution learning. Our approach achieves state-of-the-art results on popular benchmarks such as MORPH2, Adience, and the newly proposed MegaAge.Comment: To appear on BMVC 2017 (oral) revised versio

Nonplanar On-shell Diagrams and Leading Singularities of Scattering Amplitudes

Bipartite on-shell diagrams are the latest tool in constructing scattering amplitudes. In this paper we prove that a Britto-Cachazo-Feng-Witten (BCFW)-decomposable on-shell diagram process a rational top-form if and only if the algebraic ideal comprised of the geometrical constraints is shifted linearly during successive BCFW integrations. With a proper geometric interpretation of the constraints in the Grassmannian manifold, the rational top-form integration contours can thus be obtained, and understood, in a straightforward way. All rational top-form integrands of arbitrary higher loops leading singularities can therefore be derived recursively, as long as the corresponding on-shell diagram is BCFW-decomposable.Comment: 13 pages with 12 figures; final version appeared in Eur.Phys.J. C77 (2017) no.2, 8

Iterative Assembly of Two Separate Polyketide Chains by the Same Single-module Bacterial Polyketide Synthase in the Biosynthesis of HSAF

HSAF (1) was isolated from the biocontrol agent Lysobacter enzymogenes (Figure 1).[1-4] This bacterial metabolite belongs to polycyclic tetramate macrolactams (PTM) that are emerging as a new class of natural products with distinct structural features. [5, 6] HSAF exhibits a potent antifungal activity and shows a novel mode of action.[1-4] The HSAF biosynthetic gene cluster contains only a single-module hybrid polyketide synthasenonribosomal peptide synthetase (PKS-NRPS), although the PTM scaffold is apparently derived from two separate hexaketide chains and an ornithine residue.[1-4] This suggests that the same PKS module would act not only iteratively, but also separately, in order to link the two hexaketide chains with the NRPS-activated ornithine to form the characteristic PTM scaffold. Recently, the Gulder group reported heterologous expression of the ikarugamycin (4) biosynthetic gene cluster in E. coli,[7] and the Zhang group reported the enzymatic mechanism for formation of the inner 5-memebered ring and demonstrated the polyketide origin of the ikarugamycin skeleton.[8] Ikarugamycin is a Streptomyces-derived PTM which has a 5,6,5-tricyclic system (Figure 1). Both the Gulder and Zhang groups showed that a three-gene cluster is sufficient for ikarugamycin biosynthesis. Despite the progress, this iterative polyketide biosynthetic mechanism had not been demonstrated using purified PKS and NRPS. In addition, HSAF has a 5,5,6-tricyclic system, and its gene cluster contains at least six genes.[3] Finally, unlike most PTM compounds, HSAF is produced by a Gramnegative bacterium, L. enzymogenes. Here, we report the heterologous production of HSAF analogs in Gram-positive Streptomyces hosts, in which the native PKS have been deleted. We also obtained evidence for the formation of the polyene tetramate intermediate in Streptomyces when only the single-module hybrid PKS-NRPS gene was expressed. Finally, we showed the in vitro production of the polyene tetramate using the individually purified PKS and NRPS. The results provide direct evidence for this iterative polyketide biosynthetic mechanism that is likely general for the PTM-type hybrid polyketide-peptides

UAV photogrammetry in intertidal mudflats: accuracy, efficiency, and potential for integration with satellite imagery

The rapid, up-to-date, cost-effective acquisition and tracking of intertidal topography are the fundamental basis for timely, high-priority protection and restoration of the intertidal zone. The low cost, ease of use, and flexible UAV-based photogrammetry have revolutionized the monitoring of intertidal zones. However, the capability of the RTK-assisted UAV photogrammetry without ground control points, the impact of flight configuration difference, the presence of surface water in low-lying intertidal areas on the photogrammetric accuracy, and the potential of UAV/satellite Synergy remain unknown. In this paper, we used an RTK-assisted UAV to assess the impact of the above-mentioned considerations quantitatively on photogrammetric results in the context of annual monitoring of the Chongming Dongtan Nature Reserve, China based on an optimal flight combination. The results suggested that (1) RTK-assisted UAVs can obtain high-accuracy topographic data with a vertical RMSE of 3.1 cm, without the need for ground control points. (2) The effect of flight altitude on topographic accuracy was most significant and also nonlinear. (3) The elevation obtained by UAV photogrammetry was overestimated by approximately 2.4 cm in the low-lying water-bearing regions. (4) The integration of UAV and satellite observations can increase the accuracy of satellite-based waterline methods by 51%. These quantitative results not only provide scientific insights and guidelines for the balance between accuracy and efficiency in utilizing UAV-based intertidal monitoring, but also demonstrate the great potential of combined UAV and satellite observations in identifying coastal erosion hotspots. This establishes high-priority protection mechanisms and promotes coastal restoration

Biosynthesis of HSAF, a Tetramic Acid-containing Macrolactam from \u3ci\u3eLysobacter enzymogenes\u3c/i\u3e

HSAF was isolated from Lysobacter enzymogenes, a bacterium used in the biological control of fungal diseases of plants. Structurally, it is a tetramic acid-containing macrolactam fused to a tricyclic system. HSAF exhibits a novel mode of action by disrupting sphingolipids important to the polarized growth of filamentous fungi. Here, we described the HSAF biosynthetic gene cluster which contains only a single-module polyketide synthase-nonribosomal peptide synthetase (PKS/ NRPS), although the biosynthesis of HSAF apparently requires two separate polyketide chains that are linked together by one amino acid (ornithine) via two amide bonds. Flanking the PKS/ NRPS are six genes, encoding a cascade of four tightly clustered redox enzymes on one side and a sterol desaturase/fatty acid hydroxylase and a ferredoxin reductase on the other side. The genetic data demonstrate that the four redox genes, in addition to the PKS/NRPS gene and the sterol desaturase/fatty acid hydroxylase gene, are required for HSAF production. The biochemical data show that the adenylation domain of the NRPS specifically activated L-ornithine and the fourdomain NRPS was able to catalyze the formation of a tetramic acid-containing product from acyl- S-ACP and ornithinyl-S-NRPS. These results reveal a previously unrecognized biosynthetic mechanism for hybrid PK/NRP in prokaryotic organisms

Biosynthesis of HSAF, a Tetramic Acid-containing Macrolactam from \u3ci\u3eLysobacter enzymogenes\u3c/i\u3e

HSAF was isolated from Lysobacter enzymogenes, a bacterium used in the biological control of fungal diseases of plants. Structurally, it is a tetramic acid-containing macrolactam fused to a tricyclic system. HSAF exhibits a novel mode of action by disrupting sphingolipids important to the polarized growth of filamentous fungi. Here, we described the HSAF biosynthetic gene cluster which contains only a single-module polyketide synthase-nonribosomal peptide synthetase (PKS/ NRPS), although the biosynthesis of HSAF apparently requires two separate polyketide chains that are linked together by one amino acid (ornithine) via two amide bonds. Flanking the PKS/ NRPS are six genes, encoding a cascade of four tightly clustered redox enzymes on one side and a sterol desaturase/fatty acid hydroxylase and a ferredoxin reductase on the other side. The genetic data demonstrate that the four redox genes, in addition to the PKS/NRPS gene and the sterol desaturase/fatty acid hydroxylase gene, are required for HSAF production. The biochemical data show that the adenylation domain of the NRPS specifically activated L-ornithine and the fourdomain NRPS was able to catalyze the formation of a tetramic acid-containing product from acyl- S-ACP and ornithinyl-S-NRPS. These results reveal a previously unrecognized biosynthetic mechanism for hybrid PK/NRP in prokaryotic organisms