The effects of energetic frustrations introduced at different locations.

<p>Comparison of residual φ-value distributions derived from the conventional Gō-like and those from the frustrated Gō-like model for the five Im9 domains; the difference in residual φ-value changes can be ascribed to the difference of the local environments of the mutation centers. A) <b>Im9</b>. B) <b>H5A</b>. C) <b>E41A</b>. D) <b>D51A</b>. E) <b>R75A</b>.</p

Representative native contact number between secondary structures of E41A.

<p>Representative native contact number between secondary structures of E41A.</p

Representative native contact number between secondary structures of E41A.

<p>Representative native contact number between secondary structures of E41A.</p

Superposition of selected Im9 domain structures and the native-contact network.

<p>A) Structural overlap of the selected four homologous Im9 domains: d1emva_(<b>Im9</b>), green; d2gyka1(<b>D51A</b>), yellow; d1fr2a_(<b>E41A</b>), orange; d1bxia_(<b>H5A</b>), red. B) The native-contact network of <b>Im9</b> (the four mutation sites from D51A, E41A, H5A, R75A are shown in a CPK format). This figure was prepared using VMD software <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0087719#pone.0087719-Humphrey1" target="_blank">[68]</a>.</p

Apparent folding free energy changes for the five Im9 domain structures.

<p>A) <b>Im9</b>. B) <b>H5A</b>. C) <b>E41A</b>. D) <b>D51A</b>. E) <b>R75A</b>.</p

Sequence alignment of the five selected Im9 domains selected from SCOP.

<p>The abbreviations reads <b>Im9</b>: d1emva_, <b>D51A</b>: d2gyka1, <b>E41A</b>: d1fr2a_, <b>H5A</b>: d1bxia_, <b>R75A</b> :d1emvax.</p

Representative native contact number between secondary structures of H5A.

<p>Representative native contact number between secondary structures of H5A.</p

φ-value increment as a function of secondary structure for the five Im9 domains.

<p>φ-value increment as a function of secondary structure for the five Im9 domains.</p

Averaged residual φ-value increments due to the frustrations for the 5 Im9 domains, in brackets listed the standard deviations.

<p>Averaged residual φ-value increments due to the frustrations for the 5 Im9 domains, in brackets listed the standard deviations.</p

Representative native contact number between secondary structures of R75A.

<p>Representative native contact number between secondary structures of R75A.</p