Effects of sinomenine on the expression of microRNA-155 in 2,4,6-trinitrobenzenesulfonic acid-induced colitis in mice.

BackgroundSinomenine, a pure alkaloid isolated in Chinese medicine from the root of Sinomenium acutum, has been demonstrated to have anti-inflammatory and immunosuppressive effects. MicroRNAs (miRNAs) are gradually being recognized as critical mediators of disease pathogenesis via coordinated regulation of molecular effector pathways.Methodology/findingsAfter colitis was induced in mice by instillation of 5% (w/v) 2,4,6-trinitrobenzenesulfonic acid (TNBS), sinomenine at a dose of 100 or 200 mg/kg was orally administered once daily for 7 days. We evaluated body weight, survival rate, diarrhea score, histological score and myeloperoxidase (MPO) activity. The mRNA and protein expression levels of miR-155, c-Maf, TNF-α and IFN-γ were determined by quantitative RT-PCR and immunohistochemistry, respectively. Sinomenine (100 or 200 mg/kg)-treated mice with TNBS-induced colitis were significantly improved in terms of body weight, survival rate, diarrhea score, histological score and MPO activity compared with untreated mice. Both dosages of sinomenine significantly decreased the mRNA and protein expression levels of c-Maf, TNF-α and IFN-γ, which elevated in TNBS-induced colitis. Furthermore, sinomenine at a dose of 200 mg/kg significantly decreased the level of miR-155 expression by 71% (p = 0.025) compared with untreated TNBS-induced colitis in mice.Conclusions/significanceOur study evaluated the effects and potential mechanisms of sinomenine in the anti-inflammatory response via miRNA-155 in mice with TNBS-induced colitis. Our findings suggest that sinomenine has anti-inflammatory effects on TNBS-induced colitis by down-regulating the levels of miR-155 and several related inflammatory cytokines

Regional Vicarious Calibration of the SWIR-Based Atmospheric Correction Approach for MODIS-Aqua Measurements of Highly Turbid Inland Water

Water color remote sensing requires accurate atmospheric correction but this remains a significant challenge in highly turbid waters. In this respect, the shortwave infrared (SWIR) band-based atmospheric correction approach has proven advantageous when applied to the moderate resolution imaging spectroradiometer (MODIS) onboard the Aqua satellite. However, even so, uncertainties affect its accuracy. We performed a regional vicarious calibration of the MODIS-Aqua SWIR (1240, 2130)-based atmospheric correction using in situ water surface reflectance data measured during different seasons in Lake Taihu, a highly turbid lake. We then verified the accuracy of the (1240, 2130)-based atmospheric correction approach using these results; good results were obtained for the remote sensing reflectance retrievals at the 555, 645, and 859 nm, with average relative errors of 15%, 14%, and 22%, respectively, and no significant bias. Comparisons with the (1240, 2130)-based iterative approach and (1640, 2130)-based approach showed that the vicarious calibrated (1240, 2130)-based approach has the best accuracy and robustness. Thus, it is applicable to the highly turbid Lake Taihu. It may also be applicable to other highly turbid inland waters with similar optical and aerosol optical properties above water, but such applications will require further validation

CCL25/CCR9 interactions regulate the function of iNKT cells in oxazolone-induced colitis in mice.

Natural killer T (NKT) cells share phenotypic and functional properties with both conventional natural killer cells and T cells. These cells might have an important role in the pathogenesis of ulcerative colitis (UC). The interaction of chemokine ligand 25 (CCL25) with chemokine receptor 9 (CCR9) is involved in gut-specific migration of leukocytes and induces regulatory T cells (Tregs) to migrate to the intestine in chronic ileitis.In UC patients, NKT receptor CD161, CCL25, and CCR9 expression levels were evaluated by qRT-PCR. A murine model of oxazolone-induced colitis was induced in BALB/c mice. The mRNA levels of NK1.1, CCL25 and CCR9, and pro-inflammatory cytokines in mice were evaluated. The CCR9 expression on Type I or invariant NKT (iNKT) cells, and the iNKT cells chemotaxis are observed according to flow cytometry. NKT receptor CD161, CCL25 and CCR9 expression levels were significantly increased in UC patients. And, the mRNA expression levels of NK1.1, CCL25 and CCR9 were increased in oxazolone-induced colitis in mice. The production of pro-inflammatory cytokines was significantly increased, especially interleukin 4 (IL-4), IL-10 and IL-13. We observed significantly increased CCR9 expression on iNKT cells. Furthermore, we found an increased iNKT population and enhanced chemotaxis during oxazolone-induced colitis.Our study suggests that CCL25/CCR9 interactions may promote the induction and function of iNKT cells during oxazolone-induced colitis. These findings may have important implications for UC treatment and suggest a role for CCR9 inhibitors

The bacterial community significantly promotes cast iron corrosion in reclaimed wastewater distribution systems

Abstract Background Currently, the effect of the bacterial community on cast iron corrosion process does not reach consensus. Moreover, some studies have produced contrasting results, suggesting that bacteria can either accelerate or inhibit corrosion. Results The long-term effects of the bacterial community on cast iron corrosion in reclaimed wastewater distribution systems were investigated from both spatial (yellow layer vs. black layer) and temporal (1-year dynamic process) dimensions of the iron coupon-reclaimed wastewater microcosm using high-throughput sequencing and flow cytometry approaches. Cast iron coupons in the NONdisinfection and UVdisinfection reactors suffered more severe corrosion than did those in the NaClOdisinfection reactor. The bacterial community significantly promoted cast iron corrosion, which was quantified for the first time in the practical reclaimed wastewater and found to account for at least 30.5% ± 9.7% of the total weight loss. The partition of yellow and black layers of cast iron corrosion provided more accurate information on morphology and crystal structures for corrosion scales. The black layer was dense, and the particles looked fusiform, while the yellow layer was loose, and the particles were ellipse or spherical. Goethite was the predominant crystalline phase in black layers, while corrosion products mainly existed as an amorphous phase in yellow layers. The bacterial community compositions of black layers were distinctly separated from yellow layers regardless of disinfection methods. The NONdisinfection and UVdisinfection reactors had a more similar microbial composition and variation tendency for the same layer type than did the NaClOdisinfection reactor. Biofilm development can be divided into the initial start-up stage, mid-term development stage, and terminal stable stage. In total, 12 potential functional genera were selected to establish a cycle model for Fe, N, and S metabolism. Desulfovibrio was considered to accelerate the transfer of Fe0 to Fe2+ and speed up weight loss. Conclusion The long-term effect of disinfection processes on corrosion behaviors of cast iron in reclaimed wastewater distribution systems and the hidden mechanisms were deciphered for the first time. This study established a cycle model for Fe, N, and S metabolism that involved 12 functional genera and discovered the significant contribution of Desulfovibrio in promoting corrosion

The gene-specific primer list in the study.

<p>The gene-specific primer list in the study.</p

Reduction of miR-155, c-Maf, TNF-α and IFN-γ mRNA by sinomenine.

<p>Both dosages of sinomenine down-regulated the increased expression levels of miR-155 (<b>A</b>), c-Maf (<b>B</b>), TNF-α (<b>C</b>) and IFN-γ (<b>D</b>) which were induced by TNBS. n = 6 per group, *<i>p</i><0.05 versus the vehicle group and ^#<i>p</i><0.05 versus the TNBS group.</p

Effects on histological analysis and MPO activity measurement by sinomenine.

<p>Slices were inspected by a certified pathologist and the inflammatory severity of colonic tissues from survived mice in each group was scored and filmed. Samples from mice that were given sinomenine exhibited minor inflammatory reaction when compared with the TNBS group. <b>A.</b> Representative cross sections of the transversing colon. Magnifications of the images are 100-fold and 400-fold. Scale bar, 100 µm. <b>B.</b> Histological scores are depicted as means ± SEM. <b>C. </b><b>Effect of sinomenine on MPO activity in TNBS-induced colitis.</b> Sinomenine significantly reduced the increased MPO activity in colon tissues induced by TNBS when compared with the vehicle. n = 6 per group, *<i>p</i><0.05 versus the vehicle group and ^#<i>p</i><0.05 versus the TNBS group.</p