The identity of amino acid between the Mangshi virus (DH13M041) and other <i>Seadornaviruses</i>.

<p>Note</p><p>*VP1–VP12 corresponding to Seg-1 to Seg-12 of the Mangshi virus (DH13M041).</p><p>The identity of amino acid between the Mangshi virus (DH13M041) and other <i>Seadornaviruses</i>.</p

PCR Identification of DH13M041 virus in the culture supernatant of C6/36 cells using primers derived from segment 12 of BAV by 1% agarose gel electrophoresis (AGE).

<p>1: DH13M127-6; 2: DH13C233-5; 3: DH13M041. 4: Negative control.</p

Alignment of the sequence of guanylyltransferases of members of the family <i>Reoviridae</i> in the <i>Mangshi virus</i> (DH13M041) VP3 with other <i>Seadornaviruses</i>.

<p>(a) Alignments of sequences of guanylyltransferases in the vicinity of the motif Kx(I/V/L)S is shown in bold characters. Similar sequences are shaded. (b) Alignments of sequences of guanylyltransferases in the vicinity of the two histidine residues (bold) involved in the guanylyltransferase activity in BAV, KDV, and LNV. Similar sequences are shaded.</p

Electrophoretic migration patterns of the dsRNA of <i>Mangshi virus</i> (DH13M041) as determined by polyacrylamide gel electrophoresis.

<p>1: DH13M127-6 (BAV); 2:C6/36 Cells control; 3: DH13C233-5 (BAV); 4:DH13M041 (<i>Mangshi virus</i>).</p

Phylogenetic analysis of complete amino acid sequences of corresponding viral proteins (VP, see Table 1) of <i>Mangshi virus</i> (strain DH13M041) using representative members of the species <i>Balaton virus</i>, <i>Banna virus</i> (BAV), <i>Kadipiro virus</i> (KDV) and <i>Liao ning virus</i> (LNV) in the genus <i>Seadornavirus</i>.

<p>(A) RNA-dependent RNA polymerase (VP1); (B) T2 layer of core/subcore (VP2); (C) protein kinase (VP8); (D) outer-coat attachment protein (VP9) of <i>Mangshi virus</i>.</p

CPE caused by <i>Mangshi virus</i> (DH13M041) on C6/36 cells 72 h postinoculation (100×).

<p>CPE caused by <i>Mangshi virus</i> (DH13M041) on C6/36 cells 72 h postinoculation (100×).</p

Lengths of dsRNA segments 1 to 12, encoded putative proteins, 5′ and 3′ NCRs of the <i>Mangshi virus</i> (DH13M041) genome.

<p>Lengths of dsRNA segments 1 to 12, encoded putative proteins, 5′ and 3′ NCRs of the <i>Mangshi virus</i> (DH13M041) genome.</p