Effects of GA₃ treatment on the expression of flavonoid biosynthesis related genes in <i>S. baicalensis</i>.

<p>RT-PCR analysis of expression of <i>SbPAL1</i>, SbPAL2, <i>SbPAL3</i>, <i>SbC4H</i>, <i>Sb4CL</i>, <i>SbCHS</i>, <i>SbGUS</i>, and <i>SbUBGAT</i> in leaves of <i>S. baicalensis</i> after spraying GA₃. Vertical bars indicate the standard deviation of three biological replicates. Asterisks indicate a significant difference at the <i>P</i><0.05 level.</p

Transactivation assay of SbMYB2 and SbMYB7.

<p>A,C, pBD-SbMYB2; B,D, pBD-SbMYB7. pGAL4 and pBD-GAL4 was used as a positive control and negative control, respectively.</p

Effects of GA₃ treatment on the expression of MYB genes in <i>S. baicalensis</i>.

<p>RT-PCR analysis of transcriptional level of <i>SbMYB2</i>, <i>SbMYB5</i>, <i>SbMYB7</i>, <i>SbMYB8</i>, <i>SbMYB12</i>, <i>SbMYB13</i>, and <i>SbMYB19</i> in leaves of <i>S. baicalensis</i> at 0, 1, 2, 3 h after spraying GA₃. Vertical bars indicate the standard deviation of three biological replicates. Asterisks indicate a significant difference at the <i>P</i><0.05 level.</p

Gel mobility shift assays for box L of the NtPAL promoter.

<p>1, <i>E.coli</i>; 2, pGEX-4T-1; 3,4, SbMYB2; 5,6, SbMYB7; 3,5, biotin labeled box L probe; 4,6, biotin labeled and unlabeled box L probes.</p

Subcellular localization of SbMYB2 and SbMYB7.

<p>The recombinant constructs of the <i>SbMYB2-GFP</i> and <i>SbMYB7-GFP</i> fusion gene and <i>GFP</i> alone were transformed into onion (<i>Allium cepa</i>) epidermal cells by particle bombardment. A, D, G, pGEM-SbMYB2; B, E, H, pGEM-SbMYB7; C, F, I, empty vector pE3025. </p

Neighbor-joining tree representing relationships among MYB proteins from <i>Scutellaria baicalensis</i>, <i>Arabidopsis</i> and Nicotiana.

<p>The proteins are clustered into 23 subgroups, which are designated with a subgroup number (e.g., S1).</p

Effects of water deficit on endogenous GAs and IAA.

<p>The GA and IAA content of leaves of <i>S. baicalensis</i> grown under 16% SWC as a control and 12% SWC as a water deficit treatment. Vertical lines indicate the standard deviation of three biological replicates. Asterisks indicate a significant difference at the <i>P</i><0.05 level.</p

Effects of water deficit on flavonoid accumulation in <i>S. baicalensis</i>.

<p>Total flavonoids and baicalin in the leaves of <i>S. baicalensis</i> grown under 16% SWC as a control (broken line) and 12% SWC as a water deficit treatment (solid line). Vertical bars indicate the standard deviation of three biological replicates. Asterisks indicate a significant difference at the <i>P</i><0.05 level.</p

Effects of water deficit on the expression of flavonoid biosynthesis genes in <i>S. baicalensis</i>.

<p>A, RT-PCR analysis of the transcript levels of interested genes; B, Quantification of the RT-PCR results. The broken line is for the 16% SWC control and the solid line is for the 12% SWC water stress treatment. Vertical bars indicate the standard deviation of three biological replicates. Asterisks indicate a significant difference at the <i>P</i><0.05 level.</p

Differentially expressed proteins which have <i>S. baicalensis</i> cDNA sequence.

a<p>Spot abundance is expressed as the ratio of intensities of up-regulated or down-regulated proteins between stress and control. -Fold changes had p values<0.05. 30 d, 50 d, and 70 d represent water stress treatment for 30,50,70 d, respectively.</p>b<p>Number of mass values matched.</p>c<p>Sequence coverage.</p>d<p>identified proteins in protein database of <i>Scutellaria baicalensis</i> in our group.</p>e<p>ns indicates no significant change of spot abundance between stress and control.</p