Whole exome sequencing identifies frequent somatic mutations in cell-cell adhesion genes in chinese patients with lung squamous cell carcinoma

Lung squamous cell carcinoma (SQCC) accounts for about 30% of all lung cancer cases. Understanding of mutational landscape for this subtype of lung cancer in Chinese patients is currently limited. We performed whole exome sequencing in samples from 100 patients with lung SQCCs to search for somatic mutations and the subsequent target capture sequencing in another 98 samples for validation. We identified 20 significantly mutated genes, including TP53, CDH10, NFE2L2 and PTEN. Pathways with frequently mutated genes included those of cell-cell adhesion/Wnt/Hippo in 76%, oxidative stress response in 21%, and phosphatidylinositol-3-OH kinase in 36% of the tested tumor samples. Mutations of Chromatin regulatory factor genes were identified at a lower frequency. In functional assays, we observed that knockdown of CDH10 promoted cell proliferation, soft-agar colony formation, cell migration and cell invasion, and overexpression of CDH10 inhibited cell proliferation. This mutational landscape of lung SQCC in Chinese patients improves our current understanding of lung carcinogenesis, early diagnosis and personalized therapy

TRY plant trait database – enhanced coverage and open access

Plant traits - the morphological, anatomical, physiological, biochemical and phenological characteristics of plants - determine how plants respond to environmental factors, affect other trophic levels, and influence ecosystem properties and their benefits and detriments to people. Plant trait data thus represent the basis for a vast area of research spanning from evolutionary biology, community and functional ecology, to biodiversity conservation, ecosystem and landscape management, restoration, biogeography and earth system modelling. Since its foundation in 2007, the TRY database of plant traits has grown continuously. It now provides unprecedented data coverage under an open access data policy and is the main plant trait database used by the research community worldwide. Increasingly, the TRY database also supports new frontiers of trait‐based plant research, including the identification of data gaps and the subsequent mobilization or measurement of new data. To support this development, in this article we evaluate the extent of the trait data compiled in TRY and analyse emerging patterns of data coverage and representativeness. Best species coverage is achieved for categorical traits - almost complete coverage for ‘plant growth form’. However, most traits relevant for ecology and vegetation modelling are characterized by continuous intraspecific variation and trait–environmental relationships. These traits have to be measured on individual plants in their respective environment. Despite unprecedented data coverage, we observe a humbling lack of completeness and representativeness of these continuous traits in many aspects. We, therefore, conclude that reducing data gaps and biases in the TRY database remains a key challenge and requires a coordinated approach to data mobilization and trait measurements. This can only be achieved in collaboration with other initiatives

Are the benefits of Mediterranean diet simply due to unsaturated fat intake?

The Mediterranean diet is highly recommended by modern nutritionists, being derived from the traditional diet of the Mediterranean countries, with a large number of fruits and vegetables, olive oil, plus an appropriate amount of wine as an important feature. This simple, light, but the nutritious diet is thought to prevent cardiovascular and other diseases. The traditional view, as well as many studies, indicated the positive effect of olive oil rich in unsaturated fatty acids on the prevention of diseases, such as cardiovascular disease. However, some of these studies seem not to be strict enough to prove these benefits. Here, we evaluated the effectiveness of unsaturated fats, as well as analyzing the effect of some other food components in the Mediterranean diet on the prevention of age-related diseases. The results show the effect of unsaturated fatty acid may be overstated and dietary fiber and antioxidants in the Mediterranean diet perhaps also play a role that cannot be ignored. Therefore, any beneficial effect cannot be attributed to a single factor such as unsaturated fatty acids. The purpose of this work is to discuss the significance of healthy diet in the general public health and occupational health perspectives; provide advice to guide the dietary structure and food selection of populations, especially for occupational groups who have to face high working stress and frequently ignore the importance of reasonable diet structure

Construction of a COVID-19 Pandemic Situation Knowledge Graph Considering Spatial Relationships: A Case Study of Guangzhou, China

The outbreak of COVID-19 (coronavirus disease 2019) has generated a large amount of spatiotemporal data. Using a knowledge graph can help to analyze the transmission relationship between cases and locate the transmission path of the pandemic, but researchers have paid little attention to the spatial relationships between geographical entities related to the pandemic. Therefore, we propose a method for constructing a pandemic situation knowledge graph of COVID-19 that considers spatial relationships. First, we created an ontology design of the pandemic data in which spatial relationships are considered. We then constructed a non-spatial relationships extraction model based on BERT and a spatial relationships extraction model based on spatial analysis theory. Second, taking the pandemic and geographic data of Guangzhou as an example, we modeled a pandemic corpus. We extracted entities and relationships based on this model, and we constructed a pandemic situation knowledge graph that considers spatial relationships. Finally, we verified the feasibility of using this method as a visualization exploratory tool in the analysis of spatial characteristics, pandemic development situation, case sources, and case relationships analysis of pandemic-related areas

Combining Dilution of Precision and Kalman Filtering for UWB Positioning in a Narrow Space

Affected by the spatial environment, the accuracy and stability of ultra-wideband (UWB) positioning in a narrow space are significantly lower than those in the general indoor environment, which limits navigation and positioning services in a complex scene. To improve the positioning accuracy and stability of a narrow space, this study proposed a positioning algorithm by combining Kalman filter (KF) and dilution of precision (DOP). Firstly, we calculated the DOP values of the target narrow space by changing the location of the test nodes throughout the space. Secondly, the initial coordinate values of the test nodes were calculated by the weighted least squares (WLS) positioning algorithm and were used as the observation values of KF. Finally, the DOP values were adaptively introduced into KF to update the coordinates of the nodes to be tested. The proposed algorithm was tested in two narrow scenes with different length–width ratios. The experimental results showed that the DOP values of the narrow space were much higher than that of the wide space. Furthermore, even if the ranging error was low, the positioning error was high in the narrow space. The proposed fusion positioning algorithm reported a higher positioning accuracy in the narrow space, and the higher DOP values of the scene, the greater the accuracy improvement of the algorithm. This study reveals that no matter how the base stations are configured, the DOP values of the narrow space are much higher than that of the wide space, thus causing larger positioning errors. The proposed positioning algorithm can effectively suppress the positioning error caused by the narrow spatial structure, so as to improve the positioning accuracy and stability

Combining Dilution of Precision and Kalman Filtering for UWB Positioning in a Narrow Space

Affected by the spatial environment, the accuracy and stability of ultra-wideband (UWB) positioning in a narrow space are significantly lower than those in the general indoor environment, which limits navigation and positioning services in a complex scene. To improve the positioning accuracy and stability of a narrow space, this study proposed a positioning algorithm by combining Kalman filter (KF) and dilution of precision (DOP). Firstly, we calculated the DOP values of the target narrow space by changing the location of the test nodes throughout the space. Secondly, the initial coordinate values of the test nodes were calculated by the weighted least squares (WLS) positioning algorithm and were used as the observation values of KF. Finally, the DOP values were adaptively introduced into KF to update the coordinates of the nodes to be tested. The proposed algorithm was tested in two narrow scenes with different length–width ratios. The experimental results showed that the DOP values of the narrow space were much higher than that of the wide space. Furthermore, even if the ranging error was low, the positioning error was high in the narrow space. The proposed fusion positioning algorithm reported a higher positioning accuracy in the narrow space, and the higher DOP values of the scene, the greater the accuracy improvement of the algorithm. This study reveals that no matter how the base stations are configured, the DOP values of the narrow space are much higher than that of the wide space, thus causing larger positioning errors. The proposed positioning algorithm can effectively suppress the positioning error caused by the narrow spatial structure, so as to improve the positioning accuracy and stability

A Non-Intrusive Cyber Physical Social Sensing Solution to People Behavior Tracking: Mechanism, Prototype, and Field Experiments

Tracking people’s behaviors is a main category of cyber physical social sensing (CPSS)-related people-centric applications. Most tracking methods utilize camera networks or sensors built into mobile devices such as global positioning system (GPS) and Bluetooth. In this article, we propose a non-intrusive wireless fidelity (Wi-Fi)-based tracking method. To show the feasibility, we target tracking people’s access behaviors in Wi-Fi networks, which has drawn a lot of interest from the academy and industry recently. Existing methods used for acquiring access traces either provide very limited visibility into media access control (MAC)-level transmission dynamics or sometimes are inflexible and costly. In this article, we present a passive CPSS system operating in a non-intrusive, flexible, and simplified manner to overcome above limitations. We have implemented the prototype on the off-the-shelf personal computer, and performed real-world deployment experiments. The experimental results show that the method is feasible, and people’s access behaviors can be correctly tracked within a one-second delay

Laser shock peening strengthens additively manufactured high-entropy alloy through novel surface grain rotation

Additive manufacturing has flourished as an advanced technique to process metals and alloys. However, this strategy usually introduces undesired defects that deteriorates the mechanical performance of structural materials. Herein laser shock peening (LSP) is proposed as an efficient strengthening approach to reshape the surface morphology of a prototypical dual-phase AlCoCrFeNi high-entropy alloy (HEA) after additive manufacturing, in which remarkable strengthening is achieved. Combined electron back scatter diffraction and transmission electron microscope characterizations reveal that the mechanical enhancement is attributed to the grain refinement and accumulation of dislocations at the impact surface. In extreme condition of LSP, the grain refinement is not accommodated by the conventional dynamic recrystallization anymore, but a novel mechanism of parental columnar grain rotation which can be rationalized by a continuum-level theory from a geometrical perspective. The new mechanism is verified by large-scale atomistic simulations which further recognizes the critical role of multiple unstable dislocation slip and amorphization in formation of smaller grains under shock. Our strategy offers a promising pathway toward polishing morphology of HEAs and thus, prohibiting the potential intrinsic defected induced-mechanical degradation of the additively manufactured metals and alloys via novel microscopic mechanism