DISCOVERING TYROSINASE INHIBITORS FROM MORUS SP. PLANTS: AN IN SILICO STUDY

Objective: This study aimed to examine Morus sp. compounds bonding mode with critical amino acid residues in the binding pocket of the enzyme TRP1 in In silico so that it can be used as a support in the design of skin-lightening cosmetics based on Morus sp. Methods: Docking is done using autodock tools software, chem office 2019, ChemDraw professional 12, autodock 4.2, discovery studio 2016. Results: Isorhamnetin has two hydrogen bonds to amino acids Met215 and Asn205. Other compounds found in Morus sp., which have hydrogen bonds with Asn205, are dihydromorin, kaempferol, quercitrin, rutin, and morusin. Conclusion: Isorhamnetin has the best potential among other compounds as a tyrosinase inhibitor by hydrogen binding to the amino acid Met215, and Asn205 has a free energy of-6.16 kcal/mol

ISOLASI α-SELULOSA, PEMBUATAN & KARAKTERISASI MIKROKRISTALIN SELULOSA (MCC) DARI LIMBAH KULIT JERUK BABY (Citrus sinensis)

Obat memiliki kebermanfaatan yang penting dalam pelayanan kesehatan yang dapat digunakan dalam meningkatkan derajat kesehatan masyarakat. Namun, industri farmasi di Indonesia masih sangat tergantung dengan bahan baku impor. Pemanfaatan buah jeruk baby java masih sangat minim. Hal ini dapat dilihat dari limbah jeruk yang hanya dibuang, yang terdiri dari kulit dan biji. Penelitian ini bertujuan untuk mendapatkan mikrokristalin selulosa dari limbah Kulit Jeruk Baby (MCCKJB), kemudian dilakukan karakterisasi dengan Avicel PH 101 sebagai bahan baku pembanding. Karakterisasi dari MCCKJB terdiri dari uji kualitatif (pemeriksaan organoleptis, identifikasi menggunakan iodinate zinc chloride, pengujian kelarutan, uji pH) dan uji sifat fisik, antara lain: penetapan titik lebur, uji sudut diam, uji laju alir, uji kerapatan, susut pengeringan, & kompresibilitas, dimana setiap uji pada karakterisasi tersebut (uji kualitatif dan uji sifat fisik) akan dibandingkan dengan senyawa pembanding komersial yaitu Avicel PH 101. Berdasarkan hasil penelitian rendemen α-selulosa sebesar 66,75% terhadap serbuk simplisia, rendemen MCCKJB sebesar 82,53% terhadap α-selulosa dan karakterisasi serbuk MCCKJB meliputi reaksi warna, organoleptis, kelarutan, dan pH menunjukkan kemiripan karakteristik dengan Avicel® PH 101 sebagai pembanding, titik lebur 270° C, sudut diam 28,86°, laju alir 1,52 g/s, kerapatan curah 0,349 g/cm3, kerapatan mampat 0,466 g/cm3, susut pengeringan 10,3%, & kompresibilitas 25%. Berdasarkan penelitian yang telah dilakukan, karakteristik serbuk MCCKJB memiliki kemiripan dengan Avicel® PH 101

NADES EXTRACT OF GEDONG MANGO LEAVES AND MULBERRY LEAVES IN SPRAY GEL AS A SUNSCREEN

Objective: This study's purpose was to formulate a spray gel containing the combination extract of gedong mango leaves and mulberry leaves as a sunscreen preparation. Methods: Each leaf was extracted by Natural Deep Eutectic Solvent-Maceration Assisted Extraction (NADES-MAE) method. The extract (gedong mango and mulberry leaves) formulated in Carbopol Spray Gel/GM-CarSG) in a ratio of GL and ML were 3:0.5 (GM-CarSG 1); 3:3 (GM-CarSG 2); and 3:6 (GM-CarSG 3). Furthermore, the spray gel was evaluated, and the SPF value was determined. Results: The physical appearance, spreadability, pH, and viscosity of GM-CarSG 1, GM-CarSG 2, and GM-CarSG 3 meet the requirements. In addition, the three preparation have a sunscreen activity, with the SPF value of GM-CarSG 1, GM-CarSG 2, and GM-CarSG 3 being 2.08+0.03; 2.78+0.05; and 3.53+0.04, respectively. Conclusion: The results showed that the combination of GL and ML extract is potentially used as a sunscreen in spray gel preparation. The best formulation is GM-CarSG 3, which has the highest SPF value among them

FORMULATION AND TESTING OF ANTI DANDRUFF HAIR TONIC LEAF EXTRACT OF GEDONG MANGO (Mangifera indica L.var.Gedong) AGAINST MUSHROOMS Pityrosporum ovale

Gedong mango plant (Mangifera indica L.var.Gedong) is a plant that has potential as herbal medicine, especially in its leaves because it contains many secondary metabolites such as flavonoids, saponins, gallic tannins, catecat tannins, quinones and steroids or triterpenoids which can be used as antioxidants, antimicrobial and dandruff. This study aims to gedong mango leaf extract concentrations of 5%, 7%, and 10% in hair tonic preparations and to test the antidandruff activity. Gedong mango leaf simplicia (Mangifera indica L.var.Gedong) was extracted using the Microwave Assisted Extraction (MAE) method with Natural Deep Eutectic Solvents (NADES) sodium acetate dan lactic acid solvents with a molar ratlactic1:3. Hair tonic preparations are made in three formulas with the active ingredient of mango gedong leaf extract at a concentration of 5%, 7%, and 10%. Evaluation of hair tonic pr, separation air tonic preparation evaluation includes organ anoleptic, homogeneity, pH, and viscosity and eststy test of hair tonic anti-dandruffas carriedtestt by the healthy method against the fungus Pityrosporhealthyvale. Hair tonic of mango gedong leaf extract with concentrations of 5%, 7%, and 10% on organoleptic observations, homogeneity, pH, and viscosity met the requirements. The hair tonic has the potential as an antidandruff because it has antidandruff activity against the fungus Pityrosporum ovale and is classified as moderate to strong