Guanxintai Exerts Protective Effects on Ischemic Cardiomyocytes by Mitigating Oxidative Stress

Oxidative stress participates in numerous myocardial pathophysiological processes and is considered a therapeutic target for myocardial ischemia and heart failure. Guanxintai (GXT), a traditional Chinese medicine, is commonly used to treat cardiovascular disease on account of its numerous beneficial physiological activities, such as dilating coronary arteries, inhibiting platelet aggregation, and reducing the serum lipid content. However, the antioxidative properties of GXT and potential underlying mechanisms remain to be established. In the present study, we investigated the protective effects of GXT on ischemic cardiomyocytes and the associated antioxidative mechanisms, both in vivo and in vitro. Notably, GXT treatment reduced the degree of cardiomyocyte injury, myocardial apoptosis, and fibrosis and partially improved cardiac function after myocardial infarction. Furthermore, GXT suppressed the level of ROS as well as expression of NADPH oxidase (NOX) and phospho-p38 mitogen-activated protein kinase (MAPK) proteins. Our results collectively suggest that the protective effects of GXT on ischemic cardiomyocytes are exerted through its antioxidative activity of NOX inhibition

SIMBIG{\rm S{\scriptsize IM}BIG}: Galaxy Clustering Analysis with the Wavelet Scattering Transform

The non-Gaussisan spatial distribution of galaxies traces the large-scale structure of the Universe and therefore constitutes a prime observable to constrain cosmological parameters. We conduct Bayesian inference of the $\Lambda$CDM parameters $\Omega_m$, $\Omega_b$, $h$, $n_s$, and $\sigma_8$ from the BOSS CMASS galaxy sample by combining the wavelet scattering transform (WST) with a simulation-based inference approach enabled by the ${\rm S{\scriptsize IM}BIG}$ forward model. We design a set of reduced WST statistics that leverage symmetries of redshift-space data. Posterior distributions are estimated with a conditional normalizing flow trained on 20,000 simulated ${\rm S{\scriptsize IM}BIG}$ galaxy catalogs with survey realism. We assess the accuracy of the posterior estimates using simulation-based calibration and quantify generalization and robustness to the change of forward model using a suite of 2,000 test simulations. When probing scales down to $k_{\rm max}=0.5~h/\text{Mpc}$, we are able to derive accurate posterior estimates that are robust to the change of forward model for all parameters, except $\sigma_8$. We mitigate the robustness issues with $\sigma_8$ by removing the WST coefficients that probe scales smaller than $k \sim 0.3~h/\text{Mpc}$. Applied to the BOSS CMASS sample, our WST analysis yields seemingly improved constraints obtained from a standard PT-based power spectrum analysis with $k_{\rm max}=0.25~h/\text{Mpc}$ for all parameters except $h$. However, we still raise concerns on these results. The observational predictions significantly vary across different normalizing flow architectures, which we interpret as a form of model misspecification. This highlights a key challenge for forward modeling approaches when using summary statistics that are sensitive to detailed model-specific or observational imprints on galaxy clustering.Comment: 11+5 pages, 8+2 figure

Organic NIR-II dyes with ultralong circulation persistence for image-guided delivery and therapy

Acknowledgments This work was partially supported by grants from the National Key R&D Program of China (2020YFA0908800), NSFC (82111530209, 81773674, 91959103, 81573383, 21763002), Shenzhen Science and Technology Research Grant (JCYJ20190808152019182), the Applied Basic Research Program of Wuhan Municipal Bureau of Science and Technology (2019020701011429), Hubei Province Scientific and Technical Innovation Key Project (2020BAB058), the Local Development Funds of Science and Technology Department of Tibet (XZ202102YD0033C, XZ202001YD0028C), and the Fundamental Research Funds for the Central Universities.Peer reviewedPublisher PD

Development and validation of medical adhesive‐related skin injury risk assessment scale at peripherally inserted central catheter insertion site in oncology patients

Abstract Aims and objectives To construct a risk assessment scale for medical adhesive‐related skin injuries (MARSI) at the peripherally inserted central catheter (PICC) insertion site in oncology patients and test its reliability and validity. Design The STARD 2015 statement guided this study. Methods Literature research and a modified Delphi method were adopted in this study. A total of 31 experts participated in two rounds of consultation to build the assessment scale. A convenient sampling method was used to select 195 oncology patients at the PICC clinic from January to June 2022. Inter‐rater reliability was used to test the reliability of the scale. Validity was evaluated using the content validity index (CVI) and predictive validity. Results After the two rounds of consultation, the assessment scale with five dimensions and 13 primary entries and 36 secondary entries was developed, and the expert authority coefficients for both were 0.90. The inter‐rater reliability was 0.968. The CVIs of the items ranged from 0.83 to 1.00. The area under the subject's work characteristic curve was 0.757, and the sensitivity and specificity of the scale were 80.0% and 65.6%, respectively, at a cutoff score of 15.5

RNA-Seq and genetic diversity analysis of faba bean (Vicia faba L.) varieties in China

Background Faba bean (Vicia faba L) is one of the most important legumes in the world. However, there is relatively little genomic information available for this species owing to its large genome. The lack of data impedes the discovery of molecular markers and subsequent genetic research in faba bean. The objective of this study was to analyze the faba bean transcriptome, and to develop simple sequence repeat (SSR) markers to determine the genetic diversity of 226 faba bean varieties derived from different regions in China. Methods Faba bean varieties with different phenotype were used in transcriptome analysis. The functions of the unigenes were analyzed using various database. SSR markers were developed and the polymorphic markers were selected to conduct genetic diversity analysis. Results A total of 92.43 Gb of sequencing data was obtained in this study, and 133,487 unigene sequences with a total length of 178,152,541 bp were assembled. A total of 5,200 SSR markers were developed on the basis of RNA-Seq analysis. Then, 200 SSR markers were used to evaluate polymorphisms. In total, 103 (51.5%) SSR markers showed significant and repeatable bands between different faba bean varieties. Clustering analysis revealed that 226 faba bean materials were divided into five groups. Genetic diversity analysis revealed that the relationship between different faba beans in China was related, especially in the same region. These results provided a valuable data resource for annotating genes to different categories and developing SSR markers

Effects of different feeding rations on the CO2 fluxes at water-air interface and energy budget of sea cucumber Apostichopus japonicus (Selenka)

Feeding ration is one of the most important factors that directly affect growth and physiology progress of sea cucumber Apostichopus japonicus. In present study, a 32-day experiment was carried out to investigate the effects of feeding ration (1%, 3%, and 7% of total body weight, named F1, F3 and F7, respectively) on growth performance, carbon allocation, energy budget and CO2 fluxes at water-air interface. Results showed the maximum specific growth rate was observed at F3, while F7 showed negative growth. And F3 exhibited the highest enzyme activities associated with respiration in respiratory tree and body wall. Carbon intake, nitrogen intake and energy intake were significantly affected by feeding ration, while energy allocation between F1 and F3 on growth and excretion were no significant difference, suggesting that increased feeding ration slightly increased the digestive burden. Compared to F1, food conversion efficiency and fecal energy of F3 were reduced, while respiration metabolizable energy was increased. Mean CO2 flux at water-air interface of F3 was significantly higher than that of F1 at noon and dusk, and mean CO2 flux of F7 was significantly lowest than other groups at all sample times. Our results revealed that feeding rations influence CO2 fluxes at water-air interface by altering physiological status, carbon content, and energy allocation for respiration metabolizable of sea cucumber. Our study provides a theoretical basis for promoting the development of efficient low-carbon aquaculture technology for sea cucumber and sustainable development of the industry

The Guandimiao Bone Assemblage (and What it Says about the Shang Economy)

The Anyang period village of Guandimiao—the only nearly completely excavated village in China of its time—is crucially important for understanding nonurban, nonelites in North China at the end of the second millennium b.c.e. Guandimiao was a small village that specialized in ceramic production, which indicates a hitherto unsuspected degree of economic specialization and integration in the Shang countryside. One line of evidence supporting an argument for economic integration between the urban center at Anyang and the countryside comes from the bone artifact assemblage recovered from the site. The Guandimiao bone artifact assemblage can be divided into four groups, each revealing a different path of production and distribution. One of these paths of production and distribution leads from this tiny village to Great Settlement Shang and its huge bone workshops some 200 km away

Continuous carbonatitic melt-fluid evolution of a REE mineralization system : Evidence from inclusions in the Maoniuping REE Deposit, Western Sichuan, China

The Maoniuping REE deposit is a world-class deposit with 1.2 Mt of REO grading on average 2.89 wt.% REO. It is the largest in the 270-km long Mianning-Dechang REE belt and is associated with Himalayan carbonatite-alkalic complexes in the eastern Indo-Asian collisional zone, Western Sichuan Province, China. The deposit is hosted by nordmarkite stocks and carbonatite sills that display a radiometric age of 40 to 24 Ma and which intruded a Yanshanian granite pluton. The 40.3 to 27.8 Ma REE mineralization occurs as vein systems hosted in nordmarkite and carbonatite with minor altered granite and rhyolite. Four ore types are recognized based on ore texture and mineral assemblage: (1) disseminated; (2) pegmatitic; (3) brecciated; and 4) stockwork (stringer) types. Five mineralizing stages are confirmed according to vein crosscutting relationships, mineral assemblage and microthermometric results, these are: 1) carbonatite stage, 2) pegmatite stage, 3) barite-bastnaesite stage, 4) later calcite stage and 5) epigenetic oxidation stage. Varied inclusion assemblages are found in fluorite, quartz, bastnaesite, barite and calcite from stages 1 through to stage 4. The dominant inclusion types include: melt, melt-fluid, CO2-rich fluid and aqueous-rich fluid inclusions. Fluid, melt-fluid and melt inclusion studies indicate that the ore-forming fluid resulted from the unmixing of carbonatite melt and carbonatitic fluid. Initial ore-forming fluids were high-temperature (600 to 850 degrees C), high-pressure (>350 MPa) and high-density supercritical orthomagmatic fluids, characterized by SO4-rich and multicomponent composition (e.g. K, Na, Ca, Ba, Sr and REE). The dominant anion is not Cl. but SO4. The evolution of the ore-forming fluid is from a melt-fluid at high temperature, through a CO2-rich fluid at high to medium temperature to aqueous-rich fluid at low temperature. REE precipitation occurred from a high to medium temperature CO2-rich fluid. The main mechanism for REE precipitation was phase separation of CO2 and aqueous fluids resulting in a decrease of temperature and pressure. (C) 2008 Elsevier B.V. All rights reserved

Transcriptome Profiling Using Single-Molecule Direct RNA Sequencing Approach for In-depth Understanding of Genes in Secondary Metabolism Pathways of Camellia sinensis

Characteristic secondary metabolites, including flavonoids, theanine and caffeine, are important components of Camellia sinensis, and their biosynthesis has attracted widespread interest. Previous studies on the biosynthesis of these major secondary metabolites using next-generation sequencing technologies limited the accurately prediction of full-length (FL) splice isoforms. Herein, we applied single-molecule sequencing to pooled tea plant tissues, to provide a more complete transcriptome of C. sinensis. Moreover, we identified 94 FL transcripts and four alternative splicing events for enzyme-coding genes involved in the biosynthesis of flavonoids, theanine and caffeine. According to the comparison between long-read isoforms and assemble transcripts, we improved the quality and accuracy of genes sequenced by short-read next-generation sequencing technology. The resulting FL transcripts, together with the improved assembled transcripts and identified alternative splicing events, enhance our understanding of genes involved in the biosynthesis of characteristic secondary metabolites in C. sinensis

Quantitative analysis of the impact of climate change and oasification on changes in net primary productivity variation in mid-Tianshan Mountains from 2001 to 2020

Net primary productivity (NPP) has been substantially changed under the intense oasification in the urban agglomerations on the northern slopes of mid-Tianshan Mountain (UANSTM) and climate change. However, the temporal variations of NPP under the oasification remain unclear, and the relative contribution of oasification and climate change on annual NPP variation is still under debate. By using remote sensing data, reanalysis data, modified Carnegie–Ames-Stanford Approach (CASA) model, and a machine learning method, we explored the spatial–temporal variation of NPP in the UANSTM region and quantified the contribution of oasification and climate change to NPP variation from 2001 to 2020. Our study indicated that: (1) the NPP presents an overall increasing trend in the most of region and the region presented decreasing trend mainly due to the cropland conversion to the urban area; (2) the oasification-dominated NPP area concentrated in the built-up land and cropland; (3) during 2001–2020, the NPP increased by about 5.4 Tg·C, and the contribution of climatic and oasification to NPP increase were quantified (73.1% and 26.9%, respectively); (4) water-related factors was the main driver of NPP variation in the UANSTM region