Normal sleep bouts are not essential for C. elegans survival and FoxO is important for compensatory changes in sleep

Additional file 6: Decreased lag-2 function does not slow vulval development. The progeny of wild type and lag-2(q420) animals raised at 25.5 °C were selected at the L4 stage, prior to lethargus entry. Vulval eversion was scored after 3 h; the percentage of animals completing vulval eversion was recorded. Significance was assessed by student’s two-tailed t-test p value < 0.5; error bars represents SEM from 3 trials. Total number of animals: wild type n = 45 and lag-2(q420) n = 42

MOESM2 of Normal sleep bouts are not essential for C. elegans survival and FoxO is important for compensatory changes in sleep

Additional file 2: daf-16 is not required for hypertonic stress resistance in osm-7; osm-11 animals. Hypertonic stress resistance was examined in young adult animals moved to 500mM NaCl NGM plates for 10 min. osm-7(tm2256) and osm-11(rt142) are complete loss of function alleles for Notch DOS family co-ligands. daf-16(mu86) is a partial loss of function allele. Loss of Notch DOS co-ligands results in resistance to hypertonic stress, based on inability to move, spontaneously or upon prodding, after 10 min on 500mM NaCl NGM plates. Partial loss of daf-16 function does not alter hypertonic resistance in these animals. n = 40 animals for all genotypes

MOESM7 of Normal sleep bouts are not essential for C. elegans survival and FoxO is important for compensatory changes in sleep

Additional file 7: Increased lag-2 expression in adults induces anachronistic sleep bouts. Animals carrying hsp::empty, hsp::lag-2 cDNA transgenes, or wild type animals were heat shocked for 1.5 h at 34 °C. After heat shock, animals were allowed to recover at 20 °C for an additional 1 h to recover from stress-induced quiescence (shown in first set of columns). Sleep was scored for all genotypes within 15 min, based on the absence of feeding and movement. Inappropriate sleep in adult animals expressing hsp::lag-2cDNA transgene was reversible, disappearing by 2 h post-heat shock. For all genotypes, n = 40 animals; error bars represent the SEM from 2 independent trials