Analysis of the effectiveness of callusogenesis on media developed on the basis of the RZ medium

There is still no consensus on the composition of nutrient media for various genotypes, and the improvement of their composition is still an urgent problem, the solution of which can significantly speed up and facilitate the breeding process, both in traditional and heterotic breeding. In this regard, the main goal of the study was to compile new variants of nutrient media and establish the relationship between changes in the components of the medium and the efficiency of callus formation. Previously, we showed the advantage of the RZ nutrient medium (Raina, Zapata. 1997) over the N nutrient medium (Nitsch, Nitsch 1969) for domestic varieties and hybrids, but to further increase the efficiency of callusogenesis, 5 variants based on the RZ nutrient medium were developed. The nutrient medium R1 was characterized by a reduced content of CaCl2·2H2O by almost three times, however, the results obtained on it were significantly better than on the initial nutrient medium RZ

The System of Neutrophil Elastase and the Plasma Level of MMP-7 in Children with Pulmonary Arterial Hypertension and Chronic Cor Pulmonale

A significant increase in the activity of neutrophil elastase (NE) and anti-NE-protection in the plasma were detected in children having bronchopulmonary dysplasia (BPD) complicated by pulmonary arterial hypertension (PAH) and chronic cor pulmonale (CCP). The changes revealed were more pronounced in patients with CCP. The plasma concentration of the NE was slightly reduced, which was probably associated with the activation of anti-NE and an increase in the α1-antitrypsin level. A gradual increase was noted in the plasma level of the matrix metalloproteinase-7 (MMP-7) in patients with an increase in the severity of the condition. In patients with cystic fibrosis (with and without CCP), the pronounced increase in the MMP-7 level was observed. In patients with cystic fibrosis (CF), even without the additional complication with PAH and CCP, the MMP-7 level was significantly higher than in those with congenital broncho-pulmonary malformations (CBPM). The difference was increased in those patients with PAH and reached a maximum in those with CCP

Analysis of the effectiveness of callusogenesis on media developed on the basis of the RZ medium

There is still no consensus on the composition of nutrient media for various genotypes, and the improvement of their composition is still an urgent problem, the solution of which can significantly speed up and facilitate the breeding process, both in traditional and heterotic breeding. In this regard, the main goal of the study was to compile new variants of nutrient media and establish the relationship between changes in the components of the medium and the efficiency of callus formation. Previously, we showed the advantage of the RZ nutrient medium (Raina, Zapata. 1997) over the N nutrient medium (Nitsch, Nitsch 1969) for domestic varieties and hybrids, but to further increase the efficiency of callusogenesis, 5 variants based on the RZ nutrient medium were developed. The nutrient medium R1 was characterized by a reduced content of CaCl2·2H2O by almost three times, however, the results obtained on it were significantly better than on the initial nutrient medium RZ

GENOTYPE-PHENOTYPE CORRELATIONS OF THE COURSE OF CYSTIC FIBROSIS IN RUSSIAN CHILDREN. THE FIRST DESCRIPTION OF ELEVEN NEW MUTATIONS

Background. Cystic fibrosis is a hereditary disease that occurs as a result of mutations in the regulator gene of chloride ion transmembrane transport (CFTR). Finding mutations in the CFTR gene is necessary for identification of the clinical features of cystic fibrosis.Objective. Our aim was to identify genotype-phenotype correlations between mutations of the first class of pathogenicity and clinical manifestations of cystic fibrosis based on studying the prevalence and structure of CFTR gene mutations.Methods. The study included children under 18 years with cystic fibrosis admitted to hospital between 2013 and 2017. Biallelic mutations in the CFTR gene were the noninclusion criterion. The CFTR gene variants were analyzed by next-generation sequencing method.Results. In 125 patients with cystic fibrosis, 59 different variants of the CFTR gene were detected, 11 of them not previously described. The most common was the deletion c.1521_1523del found in 98 (39.2%) of the 250 analyzed CFTR gene alleles and the deletion c.1545_1546del found in 22/250 (8.8%) alleles. It has been shown that the mutation c.1545_1546del, p.Y515* was more often found in children of the Chechen nation — odds ratio (OR) 139 (95% confidence interval 15–1,257). It has been established that meconium ileus, pancreatic deficiency and cirrhosis are more common in patients with mutations of the first category of pathogenicity: OR 3.9 (95% CI 1.0–15.0), 4.4 (95% CI 1.8–11.1), and 351 (95% CI 17.5–7,046), respectively. The association of CFTR gene mutations with the development of bronchiectases and polypous pancinusitis has not been found.Conclusion. Correlations between the genotype and clinical manifestations of cystic fibrosis in Russian children with CFTR gene mutations of the first class of pathogenicity have been established