Performance comparison among different detection schemes.

<p>Performance comparison among different detection schemes.</p

Channel state’s Markov model.

<p>Channel state’s Markov model.</p

Performance comparison with different noise uncertainty levels.

<p>Performance comparison with different noise uncertainty levels.</p

Illustration of energy distribution of PU and noise signals.

<p>Illustration of energy distribution of PU and noise signals.</p

The required volume of samples (<i>P</i>_<i>f</i> = 0.1, <i>P</i>_<i>d</i> = 0.9).

<p>The required volume of samples (<i>P</i>_<i>f</i> = 0.1, <i>P</i>_<i>d</i> = 0.9).</p

Analysis of the channel state.

<p>Analysis of the channel state.</p

Point-of-Care Multiplexed Assays of Nucleic Acids Using Microcapillary-based Loop-Mediated Isothermal Amplification

This report demonstrates a straightforward, robust, multiplexed and point-of-care microcapillary-based loop-mediated isothermal amplification (cLAMP) for assaying nucleic acids. This assay integrates capillaries (glass or plastic) to introduce and house sample/reagents, segments of water droplets to prevent contamination, pocket warmers to provide heat, and a hand-held flashlight for a visual readout of the fluorescent signal. The cLAMP system allows the simultaneous detection of two RNA targets of human immunodeficiency virus (HIV) from multiple plasma samples, and achieves a high sensitivity of two copies of standard plasmid. As few nucleic acid detection methods can be wholly independent of external power supply and equipment, our cLAMP holds great promise for point-of-care applications in resource-poor settings

Point-of-Care Multiplexed Assays of Nucleic Acids Using Microcapillary-based Loop-Mediated Isothermal Amplification

This report demonstrates a straightforward, robust, multiplexed and point-of-care microcapillary-based loop-mediated isothermal amplification (cLAMP) for assaying nucleic acids. This assay integrates capillaries (glass or plastic) to introduce and house sample/reagents, segments of water droplets to prevent contamination, pocket warmers to provide heat, and a hand-held flashlight for a visual readout of the fluorescent signal. The cLAMP system allows the simultaneous detection of two RNA targets of human immunodeficiency virus (HIV) from multiple plasma samples, and achieves a high sensitivity of two copies of standard plasmid. As few nucleic acid detection methods can be wholly independent of external power supply and equipment, our cLAMP holds great promise for point-of-care applications in resource-poor settings