miR-29b Psh-match suppresses pulmonary fibrosis in mice to a greater degree than miR-29b mimic.

<p>(A) In bleomycin-induced pulmonary fibroblast model mice, quantitative real-time PCR data indicates an increase in <i>Col1a1</i> mRNA expression. Expression of <i>Col1a1</i> decreased in the lungs of mice administered with miR-29b Psh-match relative to mice administered with either PBS, miR-29b scrambled control, or miR-29b mimic (*P<0.05). (B) HE and Masson’s trichrome staining showed the pronounced diffuse fibrosis on the lungs in the bleomycin-treated mice. Administration of miR-29b Psh-match suppressed pulmonary fibrosis in an established mouse model of bleomycin-induced pulmonary fibrosis (*P<0.05). High magnification images of the lungs of mice administered with either miR-29b mimic or Psh-match mice are shown in the frames. (C) Hydroxyproline content was measured as micrograms of hydroxyproline per weight in grams of the left lung’s tissues in mice.</p

Bleomycin-induced pulmonary fibrosis in mouse lungs.

<p>(A) Images of lungs from mice that were either subject to nasal administration of methylene blue (right) or not (left). (B) Fold changes in <i>Col1a1</i> mRNA showed a peak one week after bleomycin administration. (*P<0.05) (C) Histological staining by HE and Masson’s trichrome showed pronounced diffuse fibrosis in the lungs three weeks after bleomycin administration.</p

miR-29b protects fibrotic responses in NIH/3T3 cells induced with TGF-β without activating TLRs.

<p>(A) <i>Col1a1</i> mRNA expression in NIH/3T3 cells was reduced after transfection with miR-29b Psh-match compared with cells treated only with TGF-β. The degree of reduction in <i>COL1A1</i> expression induced by miR-29b Psh-match transfection was greater than that induced by either miR-29b scrambled control or miR-29b mimic (Fig 2A) (*P<0.05). (B) miR-29b Psh-match does not induce TLR signaling activity. TLR signaling activity was measured using an NF-κB reporter in which luciferase cDNA was placed under the control of the κB response element. The dsRNA and ssRNA induce the activation of NF-κB through TLR3 and TLR7, respectively.</p

Generalized Estimating Equation Regression Analyses for Factors associated with Visual Function in Aquaporin-4 Antibody-Negative Eyes.

<p>Generalized Estimating Equation Regression Analyses for Factors associated with Visual Function in Aquaporin-4 Antibody-Negative Eyes.</p

Generalized Estimating Equation Regression Analyses for Optical Coherence Tomography Parameters in Aquaporin-4 Antibody-Positive Eyes.

<p>Generalized Estimating Equation Regression Analyses for Optical Coherence Tomography Parameters in Aquaporin-4 Antibody-Positive Eyes.</p

Retinal Structure Correlations with Ab Titer in the AQP4 Ab-Positive Group.

<p>Retinal Structure Correlations with Ab Titer in the AQP4 Ab-Positive Group.</p

Structure−function relationships in all eyes.

<p>Scatter plots of mean deviation (MD) against circumpapillary retinal nerve fiber layer (RNFL) average thickness (A), MD against ganglion cell complex (GCC) average thickness (B), logMAR visual acuity (VA) against circumpapillary RNFL average thickness (C), and VA against GCC average thickness (D). Open circles and dotted lines indicate eyes with aquaporin 4 antibody (AQP4 Ab)-negative optic neuritis, whereas closed circles and solid lines indicate eyes with antibody-positive optic neuritis. Spearman rank correlation coefficients (95% confidence interval, P-value) for the AQP4 Ab-negative and positive-groups were 0.33 (-0.22 to 0.72, 0.23) and 0.74 (0.44 to 0.89, 0.0002), respectively, between MD and average RNFL thickness (A), 0.49 (-0.03 to 0.80, 0.06) and 0.73 (0.43 to 0.89, 0.0002), respectively, between MD and average GCC thickness (B), -0.57 (-0.84 to -0.07, 0.03) and -0.40 (-0.71 to 0.06, 0.08), respectively, between logMAR VA and average RNFL thickness (C), and -0.69 (-0.89 to -0.27, 0.005) and -0.52 (-0.78 to -0.10, 0.02), respectively, between logMAR VA and average GCC thickness (D).</p

Receiver Operating Characteristic Curve Analyses for Factors Discriminating AQP4 Ab Positivity.

<p>Receiver Operating Characteristic Curve Analyses for Factors Discriminating AQP4 Ab Positivity.</p

Generalized Estimating Equation Regression Analyses for Factors associated with Visual Function in Aquaporin-4 Antibody-Positive Eyes.

<p>Generalized Estimating Equation Regression Analyses for Factors associated with Visual Function in Aquaporin-4 Antibody-Positive Eyes.</p

Structure−function relationships in eyes with single-episode optic neuritis.

<p>Scatter plots of mean deviation (MD) against circumpapillary retinal nerve fiber layer (RNFL) average thickness (A), MD against ganglion cell complex (GCC) thickness (B), logMAR visual acuity (VA) against circumpapillary RNFL average thickness (C), and VA against GCC average thickness (D). Open circles and dotted lines indicate eyes with aquaporin 4 antibody (AQP4 Ab)-negative optic neuritis, whereas closed circles and solid lines indicate eyes with antibody-positive optic neuritis. Spearman rank correlation coefficients (95% confidence interval, P-value) for the antibody-negative and -positive groups were 0.39 (-0.24 to 0.79, 0.22) and 0.75 (0.27 to 0.93, 0.008), respectively, between MD and average RNFL thickness (A), 0.58 (0.01 to 0.87, 0.05) and 0.77 (0.32 to 0.94, 0.006), respectively, between MD and average GCC thickness (B), -0.47 (-0.82 to 0.14, 0.12) and -0.39 (-0.80 to 0.28, 0.24), respectively, between logMAR VA and average RNFL thickness (C), and -0.65 (-0.89 to -0.11, 0.02) and -0.62 (-0.89 to -0.02, 0.04), respectively, between logMAR VA and average GCC thickness (D).</p