A schematic drawing showing a model of the repair for TMZ and ACNU induced DNA damage.

<p>A schematic drawing showing a model of the repair for TMZ and ACNU induced DNA damage.</p

siRNA silencing of <i>FANCD1/BRCA2</i> in glioblastoma A172 cells after ACNU and TMZ treatments.

<p><b>a</b>, expression analysis of FANCD1/BRCA2 in cells transfected with siRNA for human <i>FANCD1/BRCA2</i> or the negative control siRNA using Western blots. Actin was used as a loading control, and the relative ratios of protein were normalized using actin levels. <b>b</b>, effect of siRNA silencing of <i>FANCD1/BRCA2</i> on cellular sensitivity to ACNU and TMZ. <b>c</b>, effect of siRNA silencing of <i>FANCD1/BRCA2</i> on cell growth after ACNU or TMZ treatment. Open columns, negative control siRNA; closed columns, <i>FANCD1/BRCA2</i> siRNA. Columns show the mean of three independent experiments; the bars indicate the SD. An asterisk (*) indicates significant difference (<i>P</i><0.05). Two asterisks (**), <i>P</i><0.01; three asterisks (***), <i>P</i><0.001.</p

Rad51 foci formation in glioblastoma A172 cells after ACNU and TMZ treatments.

<p><b>a</b>, typical photo. <b>b</b>, open columns, negative control siRNA; closed columns, <i>FANCD1/BRCA2</i> siRNA. Columns show the mean of two independent experiments; bars indicate the SD.</p

Sensitivity to TMZ.

<p><b>a</b>, <i>FANCwt</i> cells (open circles), <i>FANCA^−/−C^−/−</i> cells (closed triangles), <i>FANCC^−/−</i> cells (closed squares), and <i>FANCA^−/−</i> cells (closed circles); <b>b</b>, <i>FANCwt</i> cells (open circles) and <i>FANCD2^−/−</i> cells (closed circles); <b>c</b>, <i>FANCGwt</i> cells (open circles), <i>FANCGrev</i> cells (closed triangles), and <i>FANCG^−/−</i> cells (closed circles); <b>d</b>, <i>FANCD1wt</i> cells (open circles), <i>FANCD1rev</i> cells (closed triangles), and <i>FANCD1mt</i> cells (closed circles). Each point represents the mean of three independent experiments; bars indicate the SD.</p

Relative <i>D₅₀</i> values (% compared to proficient cells) for ACNU (open columns) and TMZ (closed columns).

<p>Two asterisks (**), <i>P</i><0.01; three asterisks (***), <i>P</i><0.001.</p