46 research outputs found
The RhoA-ROCK pathway is essential for T3SS2-dependent stress fiber formation.
<p>(A) Schematic model of stress fiber formation via the RhoA-ROCK pathway and the inhibitors/siRNAs used in this study HeLa cells were treated with 10 μμM ROCK inhibitor Y27632 for 1 h (B) or 2 μμg/mL RhoA inhibitor I for 2 h (C) prior to infection. Next, the cells were infected with POR-2 (a <i>tdhAS</i>- and T3SS1-deficient strain) for 3 h, fixed, and stained to detect F-actin with Alexa-488 phalloidin. (D) Silencing of RhoA by siRNA transfection. At 24 h after siRNA transfection, HeLa cells were infected with POR-2 for 3 h. The cells were then fixed and stained with Alexa-488 phalloidin. The silencing effect on RhoA protein expression was confirmed by western blot analysis. A scrambled target sequence siRNA was used as a negative control (siControl).</p
Interaction between the Type III Effector VopO and GEF-H1 Activates the RhoA-ROCK Pathway
<div><p><i>Vibrio parahaemolyticus</i> is an important pathogen that causes food-borne gastroenteritis in humans. The type III secretion system encoded on chromosome 2 (T3SS2) plays a critical role in the enterotoxic activity of <i>V. parahaemolyticus</i>. Previous studies have demonstrated that T3SS2 induces actin stress fibers in various epithelial cell lines during infection. This stress fiber formation is strongly related to pathogenicity, but the mechanisms that underlie T3SS2-dependent actin stress fiber formation and the main effector have not been elucidated. In this study, we identified VopO as a critical T3SS2 effector protein that activates the RhoA-ROCK pathway, which is an essential pathway for the induction of the T3SS2-dependent stress fiber formation. We also determined that GEF-H1, a RhoA guanine nucleotide exchange factor (GEF), directly binds VopO and is necessary for T3SS2-dependent stress fiber formation. The GEF-H1-binding activity of VopO via an alpha helix region correlated well with its stress fiber-inducing capacity. Furthermore, we showed that VopO is involved in the T3SS2-dependent disruption of the epithelial barrier. Thus, VopO hijacks the RhoA-ROCK pathway in a different manner compared with previously reported bacterial toxins and effectors that modulate the Rho GTPase signaling pathway.</p></div
GEF-H1, a VopO binding partner, is necessary for VopO-induced stress fiber formation.
<p>(A) Identification of GEF-H1 as a VopO binding partner using a GST pull-down assay. Purified GST-VopO was mixed with a HeLa cell lysate, and the proteins retained on the glutathione beads were separated by SDS-PAGE. The proteins were detected with anti-GEF-H1, anti-LARG, anti-Ect2, anti-RhoA, and anti-β-actin antibodies. Lane 1, total HeLa cell lysate (INPUT); lane 2, eluate from glutathione beads alone; lane 3, eluate from GST-VopO bound to glutathione beads; lane 4, cell lysate associated with glutathione beads; and lane 5, cell lysate associated with GST-VopO bound to glutathione beads. (B) Direct binding of VopO to GEF-H1. Lane 1, glutathione beads alone; lane 2, purified GST-VopO; lane 3, recombinant 3×FLAG-tagged GEF-H1 (INPUT); lane 4, recombinant 3×FLAG-tagged GEF-H1 associated with glutathione beads; and lane 5, 3×FLAG-tagged GEF-H1 associated with GST-VopO bound to glutathione beads. Proteins were detected with anti-FLAG or anti-GST antibodies. (C) Diagram showing the recombinant truncated GEF-H1 proteins and summaries of their VopO-binding properties. (D) VopO-binding activity of truncated GEF-H1 proteins. After a pull-down assay using purified GST-VopO and recombinant 3×FLAG-tagged truncated GEF-H1 proteins, as shown in (C), GEF-H1 protein was detected using anti-FLAG antibody. (E) Effects of silencing GEF-H1, LARG, or Ect2 via siRNA on T3SS2-dependent stress fiber formation. At 24 h after the siRNA transfection, the cells were infected with POR-2 for 3 h. After infection, the cells were fixed and stained to detect F-actin (green) and DNA (blue). The silencing effects on the expression of each protein were confirmed by western blotting. A scrambled target sequence was used as a negative control (siControl).</p
Table_1_Genetic characterization of multidrug-resistant Escherichia coli harboring colistin-resistant gene isolated from food animals in food supply chain.xlsx
Colistin is widely used for the prophylaxis and treatment of infectious disease in humans and livestock. However, the global food chain may actively promote the dissemination of colistin-resistant bacteria in the world. Mobile colistin-resistant (mcr) genes have spread globally, in both communities and hospitals. This study sought to genomically characterize mcr-mediated colistin resistance in 16 Escherichia coli strains isolated from retail meat samples using whole genome sequencing with short-read and long-read platforms. To assess colistin resistance and the transferability of mcr genes, antimicrobial susceptibility testing and conjugation experiments were conducted. Among the 16 isolates, 11 contained mcr-1, whereas three carried mcr-3 and two contained mcr-1 and mcr-3. All isolates had minimum inhibitory concentration (MIC) for colistin in the range 1–64 μg/mL. Notably, 15 out of the 16 isolates demonstrated successful transfer of mcr genes via conjugation, indicative of their presence on plasmids. In contrast, the KK3 strain did not exhibit such transferability. Replicon types of mcr-1-containing plasmids included IncI2 and IncX4, while IncFIB, IncFII, and IncP1 contained mcr-3. Another single strain carried mcr-1.1 on IncX4 and mcr-3.5 on IncP1. Notably, one isolate contained mcr-1.1 located on a chromosome and carrying mcr-3.1 on the IncFIB plasmid. The chromosomal location of the mcr gene may ensure a steady spread of resistance in the absence of selective pressure. Retail meat products may act as critical reservoirs of plasmid-mediated colistin resistance that has been transmitted to humans.</p
Sites et monuments : bulletin de la Société pour la protection des paysages et de l'esthétique générale de la France
juillet 19841984/07 (N106)-1984/09
A predicted α-helix region in VopO (H2) is required for both GEF-H1 binding and stress fiber formation.
<p>(A) Diagram showing the recombinant truncated VopO proteins and summaries of their stress fiber-inducing activity, as determined by transfection and infection assays, and their GEF-H1-binding properties. (B) Identification of the GEF-H1-binding domain of VopO. The GEF-H1-binding activity of truncated VopO was evaluated using a GST pull-down assay. The 3xFLAG GEF-H1 and truncated GST-VopO proteins were detected using anti-FLAG or anti-GST antibodies, respectively. The asterisk indicates GST or a breakdown product. (C) Stress fiber-inducing activity of truncated VopO proteins in transfected cells. HeLa cells were transfected with GFP-fused truncated VopO constructs. The cells were stained to detect F-actin (red) and cellular DNA (blue). (D) HeLa cells were infected with <i>vopO</i> deletion mutants that expressed truncated VopO proteins and the stress fiber-inducing activity was evaluated by staining to detect F-actin (green) and cellular DNA (blue). (E) Percentage of cells exhibiting actin stress fibers after infection with <i>vopO</i> deletion mutants expressing truncated VopO proteins. One hundred cells from several fields were analyzed by microscopy in each experiment to determine whether stress fiber formation was induced. The means ± standard errors are presented for experiments conducted in triplicate. The asterisks indicate statistically significant differences (*<i>p</i> < 0.05).</p
Identification of the stress fiber formation-inducing T3SS2 effector VopO.
<div><p>(A) HeLa cells were infected with POR-2, POR-2<i>∆vcrD2</i> (a T3SS1- and T3SS2-deficient strain), POR-2<i>∆vopO</i> (a <i>vopO</i> mutant strain derived from POR-2), or POR-2<i>∆vopO/pvopO</i> (a strain complemented with the <i>vopO</i> gene) for 3 h, or were treated with 10 mM nocodazole for 1 h. After infection or nocodazole treatment, the cells were stained to detect F-actin (green) and cellular and bacterial DNA (blue).</p>
<p>(B) G-LISA was used to evaluate the relative RhoA activation level in cells infected with isogenic <i>V. parahaemolyticus</i> mutant strains for 150 min or treated with 10 mM nocodazole for 30 min. The asterisks indicate results that differ significantly from those obtained using the parent strain (POR-2) (*<i>p</i> < 0.05). The error bars indicate the standard errors for experiments performed in triplicate.</p>
<p>(C) Phosphorylated myosin light chain (pMLC) levels were evaluated via western blot analysis of cells infected with isogenic <i>V. parahaemolyticus</i> mutant strains for 3 h or treated with 10 mM nocodazole for 1 h. The infected cell lysates were probed with anti-pMLC or anti-MLC antibodies.</p>
<p>(D) The GTP-bound (active) RhoA levels were evaluated using a rhotekin pull-down assay in cells transfected with a GFP-fused VopO-expression construct (GFP-vopO) or empty GFP vector (GFP). The precipitates (GTP-RhoA) and total cell lysates (Total-RhoA) were probed with an anti-RhoA antibody.</p>
<p>(E) The pMLC levels in cells transfected with a GFP-fused VopO-expression construct (GFP-vopO) or empty GFP vector (GFP) were evaluated by western blot analysis.</p>
<p>(F) Visualization of GFP (green), F-actin (red), and cellular DNA (blue) in cells transfected with a VopO-expression construct (GFP-vopO) or empty GFP vector (GFP). The arrowheads indicate GFP-VopO-expressing cells.</p></div
Additional file 2: Table S2. of First human case report of sepsis due to infection with Streptococcus suis serotype 31 in Thailand
Primer sets used for PCR and sequencing of cpsA and cpsB of the encapsulated S. suis serotype 31 isolated from pigs in this study. (DOCX 18 kb
Additional file 1: Table S1. of First human case report of sepsis due to infection with Streptococcus suis serotype 31 in Thailand
Primer sets used for PCR and sequencing of cps locus of the unencapsulated S. suis serotype 31 strain 43640. (DOCX 25 kb
Additional file 2: of Available, Bed-sided, Comprehensive (ABC) score to a diagnosis of Methicillin-resistant Staphylococcus aureus infection: a derivation and validation study
Comparison of the two patient cohorts. (PDF 149 kb