Zyxin is a novel interacting partner for SIRT1

<p>Abstract</p> <p>Background</p> <p>SIRT1 is a mammalian homologue of NAD+-dependent deacetylase sirtuin family. It regulates longevity in several model organisms and is involved with cell survival, differentiation, metabolism among other processes in mammalian cells. SIRT1 modulates functions of various key targets via deacetylation. Recent studies have revealed SIRT1 protects neurons from axonal degeneration or neurodegeneration. Further, SIRT1 null mice exhibit growth retardation and developmental defects, suggesting its critical roles in neurons and development.</p> <p>Results</p> <p>To identify novel binding partners for SIRT1 in the central nervous system, we performed yeast two-hybrid screening on human fetal brain cDNA library and found that zyxin is a possible binding partner. SIRT1 and zyxin transcript were both preferentially expressed in developmental mouse brain. Zyxin accumulates in the nucleus where it is co-localized with SIRT1 after treatment with leptomycin B in COS-7 cells. Furthermore, SIRT1 deacetylates zyxin, suggesting SIRT1 could interact with nuclear-accumulated zyxin and modulate its function through deacetylation.</p> <p>Conclusion</p> <p>Zyxin could be a novel interacting partner of SIRT1. Zyxin is an adaptor protein at focal adhesion plaque, regulating cytoskeletal dynamics and signal transduction to convey signal from the ECM (extracellular matrix) to the nucleus. Our results raise the possibility that SIRT1 regulates signal transmission from ECM to the nucleus by modulating the functions of zyxin via deacetylation.</p

Johdanto kuzushiji -kirjoitukseen: japanilaisten kirjoitusmerkkien historiasta

Non peer reviewe

Johdanto kuzushiji -kirjoitukseen

Non peer reviewe

L’uniformisation des productions agricoles à l’époque d’Edo : des savoirs géographiques biaisés à l’origine de crises

A partir de l’analyse d’ouvrages édités à l’attention des masses populaires, de listes de productions et de traités agricoles, l’article propose une lecture critique des politiques agricoles mises en œuvre au Japon entre le début du XVIIe siècle et la fin du XIXe siècle. Il apparaît qu’au cours de cette période le pays a encouragé une uniformisation des productions agricoles sur l’ensemble de son territoire suite à l’application de savoirs importés de la Chine des Ming (1368-1644). Or, la capitale de l’empire Ming (Nankin) était située dans une région où les conditions climatiques subtropicales humides correspondaient à celles de l’Ouest du Japon, mais certainement pas à celles du Nord-est où les effets des famines ont été paradoxalement amplifiés. Cette aberration géographique peut s’expliquer à la fois par la sociologie de l’élite politique et par l’importance des représentations alimentaires du Kansai dans l’ensemble de la société d’Edo.Using works published for a popular readership, lists of products and agricultural treaties, this article proposes a critic of the agricultural policies implemented in Japan from the early seventeenth century to the late nineteenth century. It appears that the country practiced at this time a standardized agricultural production throughout its territory following the application of a knowledge imported from Ming China (1368-1644). But the capital of the Ming Empire (Nanking) was located in a region of China where the subtropical climate conditions matched western Japan, but certainly not to the Northeast areas where the effects of famine were amplified. This geographical misunderstanding can be explained by the sociology of the Edo’s political elite and by the importance of Kansai’s food habits representations in the whole Edo society

Selective Recovery of Platinum (IV) from HCl Solutions Using 2-Ethylhexylamine as a Precipitant

The selective separation and recovery of specific platinum-group metals (PGMs) from metal mixtures is a significant challenge owing to the similarity of these metals in terms of chemical and physical properties. Among the typical PGMs (Pd, Pt, and Rh), the selective recovery of Pt prior to the recovery of Pd and Rh is in high demand. In this study, we attempted the selective precipitation of Pt(IV) from mixed-metal HCl solutions using 2-ethylhexylamine (2EHA) as a precipitant and achieved the selective precipitation of Pt(IV) from Pd(II) and Rh(III) over a wide range of HCl concentrations. Selective precipitation of Pt(IV) was also achieved from HCl solutions with high levels of base metals, such as Al, Cu, Fe, and Zn. High yields of undegraded 2EHA remaining in the HCl solution after Pt(IV) precipitation were recovered using hydrophobic porous resins. X-ray photoelectron spectroscopy and thermogravimetric measurements revealed that the Pt(IV)-containing precipitate was an ion-pair comprising one [PtCl6](2-) and two ammonium cations of 2EHA. The steric hindrance and high hydrophilicity of 2EHA suppressed the formation of Rh(III)- and Pd(II)-containing precipitates, respectively, resulting in the selective precipitation of Pt(IV)

Flight Model Development of the AGU Remote Innovative CubeSat Alert System - ARICA

We present the flight model development of the 1U CubeSat, AGU Remote Innovative CubeSat Alert system (ARICA), which is scheduled to be launched in the Japanese fiscal year 2021 as the JAXA Innovative Satellite Technology Demonstration-2 project. The main goal of ARICA is to demonstrate the real-time alert system of the transient astronomical sources using commercial satellite network devices. The development of the flight components has been finished in April 2021. The thermal vacuum test was conducted at the end of April 2021. The vibration and shock tests were performed in May 2021. We are currently in the final stage of the development of ARICA to be ready for launch

Updating International Japan Studies Education with ICT Tools: A Problem-based Learning Approach to Teaching about Akutagawa Ryunosuke's “Hana” and Japanese Modernization

This paper describes a PBL-based active learning approach using ICT tools for Japanese education that is appropriate for the digital age. Based on the existing literature on PBL, we designed a two-week PBL program in which a total of eight learners in three distinct countries (Japan, Spain, and China) collaboratively studied and read Hana (“The Nose”), a short story published by the Japanese writer Ryunosuke Akutagawa, to gain insight into the nature of Japanese modernization. Despite their limited prior exposure to Japanese classics, all learners clearly demonstrated a recognizable improvement in their understanding of the text at the end of the PBL program through the mid and final presentations. They also provided positive evaluations of their PBL experiences. Some remaining issues are discussed based on these data and instructors’ reflections on PBL

Towards Generation of Visual Attention Map for Source Code

Program comprehension is a dominant process in software development and maintenance. Experts are considered to comprehend the source code efficiently by directing their gaze, or attention, to important components in it. However, reflecting the importance of components is still a remaining issue in gaze behavior analysis for source code comprehension. Here we show a conceptual framework to compare the quantified importance of source code components with the gaze behavior of programmers. We use "attention" in attention models (e.g., code2vec) as the importance indices for source code components and evaluate programmers' gaze locations based on the quantified importance. In this report, we introduce the idea of our gaze behavior analysis using the attention map, and the results of a preliminary experiment.Comment: 4 pages, 2 figures; APSIPA 2019 ACCEPTE