4 research outputs found
ゾル−ゲル転移を示す生体適合ポリマー材料の開発と応用 (2)
(1) Title: Bulk pH Responsive DNA Quadruplex Hydrogels Prepared by Liquid-Phase Large Scale DNA SynthesisJournal: ACS Macro Letters(2) Title: Communication—DNA Quadruplex Hydrogel Beads Showing Peroxidase ActivityJournal: Journal of The Electrochemical SocietyDOI: http://dx.doi.org/10.1149/2.0441909je
Application of DNA Quadruplex Hydrogels Prepared from Polyethylene Glycol-Oligodeoxynucleotide Conjugates to Cell Culture Media
Bulk pH-Responsive DNA Quadruplex Hydrogels Prepared by Liquid-Phase, Large-Scale DNA Synthesis
A new
pH-responsive hydrogel biomaterial, that is composed of solely
two popular biocompatible materials, oligodeoxynucleotides (ODN) and
polyethylene glycol (PEG) have been prepared. Merely five deoxycytidine
residues were elongated to the ends of linear or 4-arm PEG in ×1000
larger scale than conventional systems by using liquid-phase DNA synthesis
technique, and applied them as a macromonomer for the preparation
of hydrogels. The syntheses of the conjugates are simply elongating
ODN onto the ends of PEG as a semisolid phase substrate using standard
phosphoramidite chemistry. The resulting dC5-PEG conjugates gave quite
stable and stiff hydrogels triggered by the formation of a unique
DNA quadruplex, i-motif. Introduction of only one chemical linkage
between two linear conjugates resulted in unexpectedly high thermal
stabilities for the melting temperatures of i-motifs themselves. Nonlinearly
improved rheological properties compared to the original linear conjugates
were also observed, probably because of topological entanglement between
macromonomers of fused circles