Vector-type four-quark interaction and its impact on QCD phase structure

Effects of the vector-type four-quark interaction on QCD phase structure are investigated in the imaginary chemical potential region, by using the Polyakov-loop extended Nambu-Jona-Lasinio (PNJL) model with the extended Z3 symmetry. In the course to this end, we clarify analytically the Roberge-Weiss periodicity and symmetry properties of various quantities under the existence of a vector-type four-quark interaction. In the imaginary chemical potential region, the chiral condensate and the quark number density are sensitive to the strength of the interaction. Based on this result, we propose a possibility to determine the strength of the vector-type interaction, which largely affects QCD phase structure in the real chemical potential region, by comparing the results of lattice simulations and effective model calculations in the imaginary chemical potential region.Comment: 8 pages, 11 figure

Generation of a Mutant Mucor hiemalis Endoglycosidase That Acts on Core-fucosylated N-Glycans

Endo-β-N-acetylglucosaminidase M (Endo-M), an endoglycosidase from the fungus Mucor hiemalis, is a useful tool for chemoenzymatic synthesis of glycoconjugates, including glycoprotein-based therapeutics having a precisely defined glycoform, by virtue of its transglycosylation activity. Although Endo-M has been known to act on various N-glycans, it does not act on core-fucosylated N-glycans, which exist widely in mammalian glycoproteins, thus limiting its application. Therefore, we performed site-directed mutagenesis on Endo-M to isolate mutant enzymes that are able to act on mammalian-type core-α1,6-fucosylated glycans. Among the Endo-M mutant enzymes generated, those in which the tryptophan at position 251 was substituted with alanine or asparagine showed altered substrate specificities. Such mutant enzymes exhibited increased hydrolysis of a synthetic α1,6-fucosylated trimannosyl core structure, whereas their activity on the afucosylated form decreased. In addition, among the Trp-251 mutants, the W251N mutant was most efficient in hydrolyzing the core-fucosylated substrate. W251N mutants could act on the immunoglobulin G-derived core-fucosylated glycopeptides and human lactoferrin glycoproteins. This mutant was also capable of transferring the sialyl glycan from an activated substrate intermediate (sialyl glyco-oxazoline) onto an α1,6-fucosyl-N-acetylglucosaminyl biotin. Furthermore, the W251N mutant gained a glycosynthase-like activity when a N175Q substitution was introduced and it caused accumulation of the transglycosylation products. These findings not only give insights into the substrate recognition mechanism of glycoside hydrolase family 85 enzymes but also widen their scope of application in preparing homogeneous glycoforms of core-fucosylated glycoproteins for the production of potent glycoprotein-based therapeutics

Determination of QCD phase diagram from the imaginary chemical potential region

We test the reliability of the the Polyakov-loop extended Nambu-Jona-Lasinio (PNJL) model, comparing the model result with the lattice data at nonzero imaginary chemical potential. The PNJL model with the vector-type four-quark and scalar-type eight-quark interactions reproduces the lattice data on the pseudocritical temperatures of the deconfinement and chiral phase transitions. The QCD phase diagram in the real chemical potential region is predicted by the PNJL model. The critical endpoint survives, even if the vector-type four-quark interaction is taken into account.Comment: 8 pages, 12 figure

Muon Nuclear Data

We plan to develop a new nuclear database for muon-induced nuclear reactions (muon nuclear data). The database will consist of (1) energies and intensities of the muonic X rays, (2) lifetimes of the muonic atom, (3) production branching ratio of the residual nuclei by muon capture, (4) emission probabilities of the particles after muon capture, and (5) energy spectra of the emitted particles after muon capture. In this paper, we review the present status and current investigations for the muon nuclear data.Comment: Proceedings for Joint Symposium on Nuclear Data and PHITS in 2023, in print (JAEA-Conf series

Meson mass at real and imaginary chemical potentials

The chemical-potential dependence of pi and sigma meson masses is analyzed at both real and imaginary chemical potentials, $\mu_\mathrm{R}$ and $\mu_\mathrm{I}$, by using the Polyakov-loop extended Nambu--Jona-Lasinio (PNJL) model that possesses both the extended ${\mathbb Z}_3$ symmetry and the chiral symmetry. In the $\mu_\mathrm{I}$ region, the meson masses have the Roberge-Weiss periodicity. Assuming that the meson masses will be measured at finite $\mu_\mathrm{I}$ by lattice QCD in future, we simulate how meson masses at finite $\mu_\mathrm{R}$ are extracted from those at finite $\mu_\mathrm{I}$, and propose a reliable extraction method.Comment: 8 pages, 6 figures, typo corrected, some discussions clarified, version accepted for publication in Phys. Rev.

Characterization of spliced leader trans-splicing in a photosynthetic rhizarian amoeba, Paulinella micropora, and its possible role in functional gene transfer

Paulinella micropora is a rhizarian thecate amoeba, belonging to a photosynthetic Paulinella species group that has a unique organelle termed chromatophore, whose cyanobacterial origin is distinct from that of plant and algal chloroplasts. Because acquisition of the chromatophore was quite a recent event compared with that of the chloroplast ancestor, the Paulinella species are thought to be model organisms for studying the early process of primary endosymbiosis. To obtain insight into how endosymbiotically transferred genes acquire expression competence in the host nucleus, here we analyzed the 5′ end sequences of the mRNAs of P. micropora MYN1 strain with the aid of a cap-trapper cDNA library. As a result, we found that mRNAs of 27 genes, including endosymbiotically transferred genes, possessed the common 5′ end sequence of 28–33 bases that were posttranscriptionally added by spliced leader (SL) trans-splicing. We also found two subtypes of SL RNA genes encoded by the P. micropora MYN1 genome. Differing from the other SL trans-splicing organisms that usually possess poly(A)-less SL RNAs, this amoeba has polyadenylated SL RNAs. In this study, we characterize the SL trans-splicing of this unique organism and discuss the putative merits of SL trans-splicing in functional gene transfer and genome evolution

Latent trajectory modelling of pulmonary artery pressure in systemic sclerosis: a retrospective cohort study

OBJECTIVES: To visualise the trajectories of pulmonary arterial pressure (PAP) in systemic sclerosis (SSc) and identify the clinical phenotypes for each trajectory, by applying latent trajectory modelling for PAP repeatedly estimated by echocardiography. METHODS: This was a multicentre, retrospective cohort study conducted at four referral hospitals in Kyoto, Japan. Patients with SSc who were treated at study sites between 2008 and 2021 and who had at least three echocardiographic measurements of systolic PAP (sPAP) were included. A group-based trajectory model was applied to the change in sPAP over time, and patients were classified into distinct subgroups that followed similar trajectories. Pulmonary hypertension (PH)-free survival was compared for each trajectory. Multinomial logistic regression analysis was performed for baseline clinical characteristics associated with trajectory assignment. RESULTS: A total of 236 patients with 1097 sPAP measurements were included. We identified five trajectories: rapid progression (n=9, 3.8%), early elevation (n=30, 12.7%), middle elevation (n=54, 22.9%), late elevation (n=24, 10.2%) and low stable (n=119, 50.4%). The trajectories, in the listed order, showed progressively earlier elevation of sPAP and shorter PH-free survival. In the multinomial logistic regression analysis with the low stable as a reference, cardiac involvement was associated with rapid progression, diffuse cutaneous SSc was associated with early elevation and anti-centromere antibody was associated with middle elevation; older age of onset was associated with all three of these trajectories. CONCLUSION: The pattern of changes in PAP over time in SSc can be classified into five trajectories with distinctly different clinical characteristics and outcomes