Indoor Garden

A garden has a similar meaning in different cultures which is an expression of an individual’s or culture’s philosophy. It also has a long history in China. In the past, each Chinese scholar had an intimate garden which was a reflection of their inner world. But this garden culture was tightly connected with the lifestyle at that time. Many people living in modern society feel that they have lost their attachment to a traditional garden, which is too far away from daily life and only can be appreciated in a picture album. As a person who has a great interest in the inheritance of the past, by simplifying and abstracting the characteristic elements of the traditional Chinese garden I try to abstract basic components and combine them with scenes of daily life into installations. They are built by modern visual language but with a similar approach to creating that the ancient people used, which is called ‘see greatness from trivialness’ in the translation of Chinese. The same thinking method can connect my work with the past more deeply, making it more than simply a copy of a visual form. Clay is used as a primary material. Clay, especially porcelain, has a special meaning in Chinese culture through the ages, and we can see the constantly changing history of this material. It is pure, and delicate, and is a fusion of the memory of the past. I also use common materials and some ready-made objects. With the popularizing of high technology and mass production, these materials surround us all the time and represent a modern lifestyle. They are ubiquitous. So clay will be combined with these common materials, and be a key character to link different values, neutralizing conflicts between different materials. When these materials come together and create artworks with imagery, it becomes a bridge that connects past and present to show new aspects of daily life. I also hope these installations turn into a reflection of the psychological condition of people living in the city, and a discussion of different lifestyles. Within this fresh composition of materials, the viewers can make their own connection with history in the indoor garden

A new route to achieve high strength and high ductility compositions in Cr-Co-Ni-based medium-entropy alloys: A predictive model connecting theoretical calculations and experimental measurements

新規高強度高延性合金の開発に成功 --計算と実験の融合による合金設計法の確立--. 京都大学プレスリリース. 2023-05-25.A new route to achieve high strength and high ductility compositions in the Cr-Co-Ni medium entropy alloys (MEAs) is proposed, by controlling the solid solution hardening parameter (Mean Square Atomic Displacement, MSAD) and twinning propensity parameter (Stacking Fault Energy, SFE), respectively. The MSAD is calculated to increase with the increase in the Cr content and with the increase in the Ni/Co ratio at high Cr concentrations, while the SFE is calculated to decrease with the increase in the Cr content and with the increase in the Co/Ni ratio at high Cr concentrations. In experiment, the strength at 0 K (derived from the temperature dependence of yield stress) increases as the Cr content increases and/or as the Ni content increases for a given high Cr content, so that a linear correlation is found between the yield strength at 0 K and MSAD. The SFE also decreases as the Cr content increases and as the Co content increases for a given high Cr content. However, while the tensile elongation increases with the decrease in SFE down to SFE values of 10–12 mJ/m2, it abruptly decreases once the SFE decreases below this value due to a change in major deformation mode from deformation twinning to deformation-induced ε-martensite transformation. Based on the established connection between the theoretical calculation and experimental measurement, outstanding combinations of strength and ductility are predicted and experimentally confirmed at high Cr compositions and at a bit Ni-rich side of the Co/Ni equi-composition line. The proposed composition (around 45Cr-20Co-35Ni) exhibits a greater 0 K strength and a superior 77 K tensile ductility by 32 % and 13 %, respectively, compared to those of the equiatomic Cr-Co-Ni alloy

A user-centred collective system design approach for Smart Product-Service Systems:A case study on fitness product design

Emerging technologies have significantly contributed to the evolution of traditional product-service systems (PSS) into smart PSS. This transformation demands a fresh perspective and a more inventive design approach. In response, this study proposes a new User-Centred Collective System Design (CSD) framework and process for Smart PSS design, aiming to enhance stakeholder engagement during the entire design process, thus promoting highly effective and creative design solutions. A case study, titled ‘Next-G Smart Fitness PSS Design’, was carried out to test and implement this approach, contrasting the results of the CSD method with a designer-centred method. The outcomes showed a marked improvement in product novelty and user desirability of the design outcomes when using the proposed design framework. The proposed CSD framework could offer beneficial insights and user-centric viewpoints for practitioners dealing with complex challenges linked to smart PSS design

Benchmarking automated cell type annotation tools for single-cell ATAC-seq data

As single-cell chromatin accessibility profiling methods advance, scATAC-seq has become ever more important in the study of candidate regulatory genomic regions and their roles underlying developmental, evolutionary, and disease processes. At the same time, cell type annotation is critical in understanding the cellular composition of complex tissues and identifying potential novel cell types. However, most existing methods that can perform automated cell type annotation are designed to transfer labels from an annotated scRNA-seq data set to another scRNA-seq data set, and it is not clear whether these methods are adaptable to annotate scATAC-seq data. Several methods have been recently proposed for label transfer from scRNA-seq data to scATAC-seq data, but there is a lack of benchmarking study on the performance of these methods. Here, we evaluated the performance of five scATAC-seq annotation methods on both their classification accuracy and scalability using publicly available single-cell datasets from mouse and human tissues including brain, lung, kidney, PBMC, and BMMC. Using the BMMC data as basis, we further investigated the performance of these methods across different data sizes, mislabeling rates, sequencing depths and the number of cell types unique to scATAC-seq. Bridge integration, which is the only method that requires additional multimodal data and does not need gene activity calculation, was overall the best method and robust to changes in data size, mislabeling rate and sequencing depth. Conos was the most time and memory efficient method but performed the worst in terms of prediction accuracy. scJoint tended to assign cells to similar cell types and performed relatively poorly for complex datasets with deep annotations but performed better for datasets only with major label annotations. The performance of scGCN and Seurat v3 was moderate, but scGCN was the most time-consuming method and had the most similar performance to random classifiers for cell types unique to scATAC-seq

Dieth­yl(hy­droxy)ammonium 3-carb­oxy­benzoate

In the title molecular compound, C4H12NO+·C8H5O4 −, the N,N-dieth­yl(hy­droxy)ammonium cation (DTHA) is linked to the 3-carb­oxy­benzoate anion (HBDL) by O—H⋯O and N—H⋯O hydrogen bonds with a graph-set motif R 2 2(7). In the crystal, helical chains are formed by O—H⋯O hydrogen bonds, propagating along [010]. The crystal structure is further stabilized by π–π inter­actions between inversion-related HBDL benzene rings [centroid–centroid distance = 3.900 (4) Å] and C—H⋯O inter­actions

A highly efficient rice green tissue protoplast system for transient gene expression and studying light/chloroplast-related processes

<p>Abstract</p> <p>Background</p> <p>Plant protoplasts, a proven physiological and versatile cell system, are widely used in high-throughput analysis and functional characterization of genes. Green protoplasts have been successfully used in investigations of plant signal transduction pathways related to hormones, metabolites and environmental challenges. In rice, protoplasts are commonly prepared from suspension cultured cells or etiolated seedlings, but only a few studies have explored the use of protoplasts from rice green tissue.</p> <p>Results</p> <p>Here, we report a simplified method for isolating protoplasts from normally cultivated young rice green tissue without the need for unnecessary chemicals and a vacuum device. Transfections of the generated protoplasts with plasmids of a wide range of sizes (4.5-13 kb) and co-transfections with multiple plasmids achieved impressively high efficiencies and allowed evaluations by 1) protein immunoblotting analysis, 2) subcellular localization assays, and 3) protein-protein interaction analysis by bimolecular fluorescence complementation (BiFC) and firefly luciferase complementation (FLC). Importantly, the rice green tissue protoplasts were photosynthetically active and sensitive to the retrograde plastid signaling inducer norflurazon (NF). Transient expression of the GFP-tagged light-related transcription factor OsGLK1 markedly upregulated transcript levels of the endogeneous photosynthetic genes <it>OsLhcb1</it>, <it>OsLhcp</it>, <it>GADPH </it>and <it>RbcS</it>, which were reduced to some extent by NF treatment in the rice green tissue protoplasts.</p> <p>Conclusions</p> <p>We show here a simplified and highly efficient transient gene expression system using photosynthetically active rice green tissue protoplasts and its broad applications in protein immunoblot, localization and protein-protein interaction assays. These rice green tissue protoplasts will be particularly useful in studies of light/chloroplast-related processes.</p