COG functional annotation of putative proteins.

<p>Of 23,739 Nr hits, 8,580 sequences had COG classifications among the 25 categories. The y-axis indicates the number of sequences in a category.</p

Homology search of Illumina sequences against the Nr database.

<p><b>(A)</b> E-value distribution of BLAST hits for each unique sequence with a cut-off E-value of 1.0E^-5. <b>(B)</b> Species distribution of the top BLAST hits for each sequence. <b>(C)</b> Species distribution as a percentage of the total homologous sequences with an E-value ≥1.0E^-5. The first hit of each sequence was used for analysis.</p

“Terpenoid backbone biosynthesis” pathway of <i>M</i>. <i>cichorii de novo</i> transcriptome.

<p>The red box indicates the homologous enzyme of the unigenes have been found in the transcriptome databases of <i>M</i>. <i>cichorii</i>, while the white box represents there is no unigene has been found.</p

RT-qPCR analysis of 13 unigenes differentially-expressed between 20–25 day-old female and male groups.

<p>Line diagrams represent the expression patterns of selected unigenes from the <i>M</i>. <i>cichorii</i> transcriptome. Expression was compared between female and male groups. Error bars represent standard error. *Significant difference between gene expression (based on 20–25 day-old females, using Student’s <i>t</i>-test with p < 0.05).</p

Length distribution of assembled unigenes.

<p>The number of y-axis has been transfer into logarithmic scale.</p

Gene Ontology analysis of genes in the Illumina <i>de novo</i> transcriptome of <i>M</i>. <i>cichorii</i>.

<p>The results are summarized in three main categories: biological process, cellular component, and molecular function. The righty-axis indicates the number of genes in a category. The left y-axis indicates the percentage of a specific category of genes in that main category.</p

Association of (A) haplotypes and (B) diplotypes on 9q33.3 with risk of hepatic fibrosis in chronic S. japonicum infected Adults in the discovery study.

<p>Block 1, rs12343206–rs10733669; Block 2, rs11355458–rs17840761–rs17840762–rs391957; Block 3, rs10819146–rs531599; Block 4, rs2454217–rs487914–rs488039. RR, relative risk; CI, confidence interval. Unadjusted RR was calculated using Pearson Chi-square test. Adjusted RR was adjusted for age, gender, smoking, drinking and HBsAg. d, the deleted base.</p

Association results of the two SNPs with risk of <i>S. japonicum</i> infection in the discovery, replicative and combined studies.

<p>Adjusted for age, gender, smoking and drinking.</p><p><i>P</i><1.163×10⁻³ means significant value by Bonferroni correction based on the total number of markers genotyped.</p

Analysis of the statistical interactions between rs10118570 and rs391957 in the discovery, replicative and combined cohorts.

<p>*<i>P</i>_int, <i>P</i> value for the interaction.</p><p><i>P</i>_int<0.0167 means significant value by Bonferroni correction based on the total number of studies.</p

Variables associated with presence or absence of advanced hepatic fibrosis (grade III/IV) in the discovery, replicative and combined cohorts with univariate and multivariate analysis.

<p>(A) Analysis in the discovery cohort. (B) Analysis in the replicative cohort. (C) Analysis in the combined cohort.</p