第828回千葉医学会例会・第6回磯野外科例会 88-3.

Western blotting was performed to examine the protein levels of Twist in the indicated cells; β-actin was used as control. (JPG 151 kb

DataSheet1_Summer extreme consecutive dry days over Northeast China in the changing climate: Observed features and projected future changes based on CESM-LE.docx

Northeast China (NEC) is a major crop base in East Asia, and summer drought is one of the climate extremes that significantly influences NEC agricultural production. Therefore, understanding the response of NEC summer drought to global warming is of significance. In this study, based on observation and large-ensemble simulations of the Community Earth System Model (CESM-LE), the variabilities in summer extreme consecutive dry days (CDDs) over NEC are investigated in the present and future climate. In the observation, the NEC summer extreme CDDs showed an increasing trend during the past half century and experienced a significant interdecadal change around the middle 1990s, which is mainly due to the change in the anticyclone over Lake Baikal-Northeast Asia. The anticyclone-related anomalous downward motion and moisture divergence provided favorable conditions for increased summer CDDs over NEC. The CESM-LE multimember ensemble (MME) simulation could reproduce the change in NEC summer extreme CDDs and its related atmospheric circulations, indicating that the observed change in NEC summer extreme CDDs could be largely contributed by anthropogenic forcing. In the future warmer climate, the NEC summer extreme CDDs are projected to show interdecadal variability, which increase by approximately 6.7% in the early 21st century (2020–2030), then decrease by approximately 0.3% in the middle to late 21st century (2040–2080), and further increase by approximately 2.1% in the late 21st century (2085–2100). In addition, the projected changes in the anticyclone over Lake Baikal-Northeast Asia show a similar feature to that of the NEC summer extreme CDDs, which might further provide some confidence in the projection of the NEC summer extreme CDDs due to the physical connection between CDDs and anticyclone in the future.</p

Additional file 4: Figure S3. of Long non-coding RNA MALAT-1 modulates metastatic potential of tongue squamous cell carcinomas partially through the regulation of small proline rich proteins

WST-1 (Roche) assay measuring the activity of mitochondrial dehydrogenases was performed following the manufacturer’s instruction at 0-, 1-, 2-, 3-, 4- day time points. Error bars represent the standard deviation of the mean. (JPG 261 kb

Additional file 3: Figure S2. of Long non-coding RNA MALAT-1 modulates metastatic potential of tongue squamous cell carcinomas partially through the regulation of small proline rich proteins

Establishment of the SCC metastases animal model in nude mice; grossly obvious tumors and metastases were dissected and fixed immediately with 4 % paraformaldehyde for pathological analysis. (JPG 1505 kb

Identification of a Novel Function of Adipocyte Plasma Membrane-Associated Protein (APMAP) in Gestational Diabetes Mellitus by Proteomic Analysis of Omental Adipose Tissue

Gestational diabetes mellitus (GDM) is considered as an early stage of type 2 diabetes mellitus. In this study, we compared demographic and clinical data between six GDM subjects and six normal glucose tolerance (NGT; healthy controls) subjects and found that homeostasis model of assessment for insulin resistance index (HOMA-IR) increased in GDM. Many previous studies demonstrated that omental adipose tissue dysfunction could induce insulin resistance. Thus, to investigate the cause of insulin resistance in GDM, we used label-free proteomics to identify differentially expressed proteins in omental adipose tissues from GDM and NGT subjects (data are available via ProteomeXchange with identifier PXD003095). A total of 3528 proteins were identified, including 66 significantly changed proteins. Adipocyte plasma membrane-associated protein (APMAP, a.k.a. C20orf3), one of the differentially expressed proteins, was down-regulated in GDM omental adipose tissues. Furthermore, mature 3T3-L1 adipocytes were used to simulate omental adipocytes. The inhibition of APMAP expression by RNAi impaired insulin signaling and activated NFκB signaling in these adipocytes. Our study revealed that the down-regulation of APMAP in omental adipose tissue may play an important role in insulin resistance in the pathophysiology of GDM