Space advanced technology demonstration satellite

The Space Advanced Technology demonstration satellite (SATech-01), a mission for low-cost space science and new technology experiments, organized by Chinese Academy of Sciences (CAS), was successfully launched into a Sun-synchronous orbit at an altitude of similar to 500 km on July 27, 2022, from the Jiuquan Satellite Launch Centre. Serving as an experimental platform for space science exploration and the demonstration of advanced common technologies in orbit, SATech-01 is equipped with 16 experimental payloads, including the solar upper transition region imager (SUTRI), the lobster eye imager for astronomy (LEIA), the high energy burst searcher (HEBS), and a High Precision Magnetic Field Measurement System based on a CPT Magnetometer (CPT). It also incorporates an imager with freeform optics, an integrated thermal imaging sensor, and a multi-functional integrated imager, etc. This paper provides an overview of SATech-01, including a technical description of the satellite and its scientific payloads, along with their on-orbit performance

Weighted Radial Basis Collocation Method for the Nonlinear Inverse Helmholtz Problems

In this paper, a meshfree weighted radial basis collocation method associated with the Newton’s iteration method is introduced to solve the nonlinear inverse Helmholtz problems for identifying the parameter. All the measurement data can be included in the least-squares solution, which can avoid the iteration calculations for comparing the solutions with part of the measurement data in the Galerkin-based methods. Appropriate weights are imposed on the boundary conditions and measurement conditions to balance the errors, which leads to the high accuracy and optimal convergence for solving the inverse problems. Moreover, it is quite easy to extend the solution process of the one-dimensional inverse problem to high-dimensional inverse problem. Nonlinear numerical examples include one-, two- and three-dimensional inverse Helmholtz problems of constant and varying parameter identification in regular and irregular domains and show the high accuracy and exponential convergence of the presented method

Ontology maintenance in high level architecture federation development and execution process

This paper presents an ontology maintenance approach aiming at dynamically maintaining the federation collaboration ontology in High Level Architecture (HLA) federation development and execution process, with the objective to provide guidelines on how to use, organize and manipulate federation collaboration ontology to meet various requirements. The proposed approach includes two algorithms: ontology maintenance (+) and ontology maintenance (-), corresponding to federate joining and resigning. It adopts an axiom-based deduction strategy and takes heavy-weighted ontologies into consideration. It can find all the explicit and derived inter-ontology relations. A case study shows that the proposed approach has great potential to improve the efficiency of federation development and execution processes, reduce the workload for adaptive adjustment of existing platforms, and enhance the applicability and flexibility of HLA based collaborative product development systems. \ua9 2013 - IOS Press and the author(s).Peer reviewed: YesNRC publication: Ye

Identification and Functional Evaluation of Three Polyubiquitin Promoters from Hevea brasiliensis

Hevea brasiliensis is an economically important tree species that provides the only commercial source of natural rubber. The replacement of the CaMV35S promoter by endogenous polyubiquitin promoters may be a viable way to improve the genetic transformation of this species. However, no endogenous polyubiquitin promoters in Hevea have been reported yet. Here, we identified three Hevea polyubiquitin genes HbUBI10.1, HbUBI10.2 and HbUBI10.3, which encode ubiquitin monomers having nearly identical amino acid sequences to that of AtUBQ10. The genomic fragments upstream of these HbUBI genes, including the signature leading introns, were amplified as putative HbUBI promoters. In silico analysis showed that a number of cis-acting elements which are conserved within strong constitutive polyubiquitin promoters were presented in these HbUBI promoters. Transcriptomic data revealed that HbUBI10.1 and HbUBI10.2 had a constitutive expression in Hevea plants. Semi-quantitative RT-PCR showed that these three HbUBI genes were expressed higher than the GUS gene driven by CaMV35S in transgenic Hevea leaves. All three HbUBI promoters exhibited the capability to direct GFP expression in both transient and stable transformation assays, although they produced lower protoplast transformation efficiencies than the CaMV35S promoter. These HbUBI promoters will expand the availability of promoters for driving the transgene expression in Hevea genetic engineering

Identification, Characterization and Expression Analysis of Anthocyanin Biosynthesis-related bHLH Genes in Blueberry (Vaccinium corymbosum L.)

Basic helix-loop-helix proteins (bHLHs) play very important roles in the anthocyanin biosynthesis of many plant species. However, the reports on blueberry anthocyanin biosynthesis-related bHLHs were very limited. In this study, six anthocyanin biosynthesis-related bHLHs were identified from blueberry genome data through homologous protein sequence alignment. Among these blueberry bHLHs, VcAN1, VcbHLH42-1, VcbHLH42-2 and VcbHLH42-3 were clustered into one group, while VcbHLH1-1 and VcbHLH1-2 were clustered into the other group. All these bHLHs were of the bHLH-MYC_N domain, had DNA binding sites and reported conserved amino acids in the bHLH domain, indicating that they were all G-box binding proteins. Protein subcellular location prediction result revealed that all these bHLHs were nucleus-located. Gene structure analysis showed that VcAN1 gDNA contained eight introns, while all the others contained seven introns. Many light-, phytohormone-, stress- and plant growth and development-related cis-acting elements and transcription factor binding sites (TFBSs) were identified in their promoters, but the types and numbers of cis-elements and TFBSs varied greatly between the two bHLH groups. Quantitative real-time PCR results showed that VcAN1 expressed highly in old leaf, stem and blue fruit, and its expression increased as the blueberry fruit ripened. Its expression in purple podetium and old leaf was respectively significantly higher than in green podetium and young leaf, indicating that VcAN1 plays roles in anthocyanin biosynthesis regulation not only in fruit but also in podetium and leaf. VcbHLH1-1 expressed the highest in young leaf and stem, and the lowest in green fruit. The expression of VcbHLH1-1 also increased as the fruit ripened, and its expression in blue fruit was significantly higher than in green fruit. VcbHLH1-2 showed high expression in stem but low expression in fruit, especially in red fruit. Our study indicated that the anthocyanin biosynthesis regulatory functions of these bHLHs showed certain spatiotemporal specificity. Additionally, VcAN1 might be a key gene controlling the anthocyanin biosynthesis in blueberry, whose function is worth exploring further for its potential applications in plant high anthocyanin breeding

A DFT Study on the Catalytic CO Oxidative Coupling to Dimethyl Oxalate on Al-Doped Core–Shell Pd Clusters

A series of core–shell catalysts aiming at CO oxidative coupling to dimethyl oxalate (DMO) were constructed, and effects of the second metal doping and surface structures on the reaction activity and favorable reaction path were investigated by using the density functional theory (DFT) method. Pd₁₃, Al@Pd₁₂, and Ag@Pd₁₂ were first studied to find the proper doping metal. Our results showed that the activity of CO oxidative coupling to DMO follows the order of Al@Pd₁₂ > Pd₁₃ > Ag@Pd₁₂, and the same result was also obtained via the electronic analysis. In addition, Al₆@Pd₃₂ and Al₁₃@Pd₄₂ catalysts with higher doping ratio and lower cost than that of Al@Pd₁₂ were selected to examine the influence of surface structure on the reaction activity. It showed that CO + CH₃O → COOCH₃ + CO → OCCOOCH₃ + CH₃O → DMO is the favorable pathway on the (100) surface of Al₆@Pd₃₂ catalyst, while CO + CH₃O → CO + CH₃O (COOCH₃) → COOCH₃ + COOCH₃ → DMO is the optimal pathway on the (111) surface of Al@Pd₁₂ and Al₁₃@Pd₄₂, which indicated that the surface structure of catalysts affected the preferable pathway of DMO formation. Moreover, activities of CO oxidative coupling to DMO on AlPd core–shell catalysts followed the order of Al@Pd₁₂ > Al₁₃@Pd₄₂ > Al₆@Pd₃₂. In addition, Al₁₃@Pd₄₂ also exhibited a good selectivity between DMO and DMC. Thus, Al₁₃@Pd₄₂ is a proper catalyst with high activity, high selectivity, and low cost because of high Al:Pd ratio

Manifold learning based cross-media retrieval: A solution to media object complementary nature

Media objects of different modalities always exist jointly and they are naturally complementary of each other, either in the view of semantics or in the view of modality. In this paper, we propose a manifold learning based cross-media retrieval approach that gives solutions to the two intrinsically basic but crucial questions of media objects semantics understanding and cross-media retrieval. First, considering the semantic complementary, how can we represent the concurrent media objects and fuse the complementary information they carry to understand the integrated semantics precisely. Second, considering the modality complementary, how can we accomplish the modality bridge to establish the cross-index and facilitate the cross-media retrieval? To solve the two problems, we first construct a Multimedia Document (MMD) Semi-Semantic Graph (MMDSSG) and then adopt Multidimensional Scaling to create an MMD Semantic Space (MMDSS). Both long-term and short-term feedbacks are proposed to boost the system performance. The first one is used to refine the MMDSSG and the second one is adopted to introduce new items that are not in the training set into the MMDSS. Since all of the MMDs and their component media objects of different modalities lie in the MMDSS and they are indexed uniformly by their coordinates in the MMDSS regardless of their modalities, the semantic subspace is actually a bridge of media objects which are of different modalities and the crossmedia retrieval can be easily achieved. Experiment results are encouraging and indicate that the proposed approach is effective

Knockdown of FRAT1 expression by RNA interference inhibits human glioblastoma cell growth, migration and invasion.

BACKGROUND: FRAT1 positively regulates the Wnt/β-catenin signaling pathway by inhibiting GSK-3-mediated phosphorylation of β-catenin. It was originally characterized as a protein frequently rearranged in advanced T cell lymphoma, but has recently also been identified as a proto-oncogene involved in tumorigenesis. Our previous studies showed that FRAT1 was dramatically overexpressed in gliomas and its expression level was significantly increased along with clinicopathological grades. METHODS: In the current study, we used RT-PCR and Western blotting to assess the mRNA and protein levels of FRAT1 in three glioma cell lines. In addition, to evaluate its functional role in gliomas, we examined the effects of FRAT1 knockdown on proliferation, migration and invasion in vitro and tumor growth in vivo using glioblastoma U251 cells and RNAi. RESULTS: FRAT1 was highly expressed in all three glioma cell lines. RNAi-mediated down-regulation of endogenous FRAT1 in human glioblastoma U251 cells resulted in suppression of cell proliferation, arrest of cell cycle, inhibition of cell migration and invasion in vitro. Moreover, FRAT1 depletion significantly impaired tumor xenograft growth in nude mice. CONCLUSIONS: Our results highlight the potential role of FRAT1 in tumorigenesis and progression of glioblastoma. These findings provide a biological basis for FRAT1 as a potential molecular marker for improved pathological grading and as a novel candidate therapeutic target for glioblastoma management

Bureaucratic integration and regional specialization in China

10.1016/j.chieco.2006.11.005China Economic Review192308-31