Completion of Eight Gynostemma BL. (Cucurbitaceae) Chloroplast Genomes: Characterization, Comparative Analysis, and Phylogenetic Relationships

Gynostemma BL., belonging to the family Cucurbitaceae, is a genus containing 17 creeping herbaceous species mainly distributed in East Asia. It can be divided into two subgenera based on different fruit morphology. Herein, we report eight complete chloroplast genome sequences of the genus Gynostemma, which were obtained by Illumina paired-end sequencing, assembly, and annotation. The length of the eight complete cp genomes ranged from 157,576 bp (G. pentaphyllum) to 158,273 bp (G. laxiflorum). Each encoded 133 genes, including 87 protein-coding genes, 37 tRNA genes, eight rRNA genes, and one pseudogene. The four types of repeated sequences had been discovered and indicated that the repeated structure for species in the Subgen. Triostellum was greater than that for species in the Subgen. Gynostemma. The percentage of variation of the eight cp genomes in different regions were calculated, which demonstrated that the coding and inverted repeats regions were highly conserved. Phylogenetic analysis based on Bayesian inference and maximum likelihood methods strongly supported the phylogenetic position of the genus Gynostemma as a member of family Cucurbitaceae. The phylogenetic relationships among the eight species were clearly resolved using the complete cp genome sequences in this study. It will also provide potential molecular markers and candidate DNA barcodes for future studies and enrich the valuable complete cp genome resources of Cucurbitaceae

Artemisia pollen allergy in China : Component-resolved diagnosis reveals allergic asthma patients have significant multiple allergen sensitization

Background: Artemisia pollen allergy is a major cause of asthma in Northern China. Possible associations between IgE responses to Artemisia allergen components and clinical phenotypes have not yet been evaluated. This study was to establish sensitization patterns of four Artemisia allergens and possible associations with demographic characteristics and clinical phenotypes in three areas of China. Methods: Two hundred and forty patients allergic to Artemisia pollen were examined, 178 from Shanxi and 30 from Shandong Provinces in Northern China, and 32 from Yunnan Province in Southwestern China. Allergic asthma, rhinitis, conjunctivitis, and eczema symptoms were diagnosed. All patients sera were tested by ImmunoCAP with mugwort pollen extract and the natural components nArt v 1, nArt ar 2, nArt v 3, and nArt an 7. Results: The frequency of sensitization and the IgE levels of the four components in Artemisia allergic patients from Southwestern China were significantly lower than in those from the North. Art v 1 and Art an 7 were the most frequently recognized allergens (84% and 87%, respectively), followed by Art v 3 (66%) and Art ar 2 (48%). Patients from Northern China were more likely to have allergic asthma (50%) than patients from Southwestern China (3%), and being sensitized to more than two allergens increased the risk of allergic asthma, in which cosensitization to three major allergens Art v 1, Art v 3, and Art an 7 is prominent. Conclusions: Componentresolved diagnosis of Chinese Artemisia pollenallergic patients helps assess the potential risk of mugwortassociated allergic asthma.(VLID)329956

Characterization of the complete chloroplast genome of Viburnum farreri (Adoxaceae)

The whole chloroplast (cp) genome sequence of Viburnum farreri has been characterized from Illumina pair-end sequencing. The complete cp genome was 158,516 bp in length, containing a large single-copy region (LSC) of 86,990 bp and a small single-copy region (SSC) of 18,502 bp, which were separated by a pair of inverted repeat (IR) regions of 26,512 bp. The genome contained 130 genes, including 85 protein-coding genes, 37 tRNA genes, and 8 ribosomal RNA genes (4 rRNA species). Most genes occur as a single copy, while 17 gene species are duplicated. Phylogenetic analysis revealed that V. farreri is closely related to the species of V. brachybotryum

Design Flow of English Learning System Based on Item Response Theory

The popularity of computer technology in English teaching has led to the establishment of many English learning platforms, but the enhancement of students’ English proficiency is limited due to the lack of relevance, self-adaptive test questions and analytical ability. The project management theory is introduced into English learning, which can provide students with teaching content and test questions that are more suitable for their own actual situation through a more intelligent, personalized way. At the same time, the static and dynamic database model based on students’ own learning behavior is constructed to facilitate storage of students’ learning record. Combined with the advantages of hierarchical selection, SH method and improved polynomial model, this paper puts forward a new type of item section model. This paper introduces the basic theory and related technology, and then makes an in-depth study on the demand analysis of English learning system. Finally, this paper realizes the design of English learning system based on item response theory and validates the good effect of English item selection from the perspective of application. The system provides teachers and students with convenient learning strategies, item selection strategies, test strategies and academic performance strategies. The introduction of item response theory enables the system to become truly student-centered and provides a more comprehensive and self-adaptive learning model, which is of great significance for improving the learning efficiency of English learning and the learning efficiency of college students in China

A New Security Warning Model about Power Grid

In recent years, lots of power grid blackouts at home and abroad indicates that the meteorological disasters caused by external meteorological conditions has gradually rose to major contradiction of power grid security. Basing on release information of meteorology, lightning monitoring information and power transmission equipment monitoring information, it established a weather warning model about power grid security, combined with real-time security analysis. Firstly, mathematical models of various types of weather conditions and weather risk assessment model grid were built, then actual operating conditions of a certain regional power grid and model results were compared, the comparison result prove the accuracy of the warning model, and provides a strong recommendation for decision-making

A New Security Warning Model about Power Grid

In recent years, lots of power grid blackouts at home and abroad indicates that the meteorological disasters caused by external meteorological conditions has gradually rose to major contradiction of power grid security. Basing on release information of meteorology, lightning monitoring information and power transmission equipment monitoring information, it established a weather warning model about power grid security, combined with real-time security analysis. Firstly, mathematical models of various types of weather conditions and weather risk assessment model grid were built, then actual operating conditions of a certain regional power grid and model results were compared, the comparison result prove the accuracy of the warning model, and provides a strong recommendation for decision-making